Homoeriodictyol
Based on 1 Customer Validation
Homoeriodictyol is an orally active, bitter-tasting flavanone that can penetrate the blood-brain barrier. Homoeriodictyol enhances synaptic-related protein expression through NCOA4-mediated ferritin autophagy. Homoeriodictyol improves memory impairment in mice by inhibiting the NLRP3 inflammasome. Homoeriodictyol protects human endothelial cells from oxidative damage by activating Nrf2 and inhibiting mitochondrial dysfunction. Homoeriodictyol enhances ROS activity and induces apoptosis, exhibiting anticancer effects. Homoeriodictyol inhibits the survival and migration of androgen-resistant prostate cancer cells in vitro. Homoeriodictyol exerts antinociceptive activity in mice in vivo.
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- Pureza: 99.86%
- No. CAS: 446-71-9
- Fòrmula: C16H14O6
- Peso molecular:302.28
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Almacenamiento:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Pureza y Documentación
- Referencias
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Help & FAQs
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Actividad biológica
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Caspase 3 |
Caspase-9 |
Homoeriodictyol (0.1-1 μM) increases cell viability, PSD-95, SYN, P62, and FTH levels, and decreases LC3 and NCOA4 levels in Corticosterone (HY-B1618)-induced SH-SY5Y cells[1].
Homoeriodictyol (10 μM/L, 24 h) restores cell viability and migration levels, and reduces inflammatory factor levels in N9 microglial cells[2].
Homoeriodictyol (0-20 μM, 16 h) upregulates the protein levels of Nrf2 and γGCS in MDA-MB-231 cells[3].
Homoeriodictyol (1-5 μM, 12-24 h) prevents mitochondrial dysfunction and apoptosis, reduces MMP, cytochrome C, tAIF, cleaved caspase -3, -9 and PARP levels in H2O2-induced EA.hy926 cells
[3].
Homoeriodictyol (0-5 μM, 2-24 h) activats the Nrf2 pathway through inducing Nrf2 at the protein level and enhancing Nrf2 stability in EA.hy926 cells[3].
Homoeriodictyol (100-500 μg/mL, 24 h) shows cytotoxicity and concentration-dependent lysosomal toxicity, enhances intracellular ROS generation and induces mitochondrial dysfunction in MCF-7, HeLa, and HT-29 cells[4].
Homoeriodictyol (500 μg/mL, 24 h) increases the subG1 peaks, increases the expressions of p53, caspase 3, caspase 9, bax, and SOD1 genes in MCF-7, HeLa, and HT-29 cells[4].
Homoeriodictyol (0-250 μM, 24-72 h) reduced cell viability against PC3 cells, with an IC50 of 118.15 μM, induces apoptosis, inhibits cell migration in PC3 cells[6].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:N9 microglial cells
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Concentration:10 μM/L
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Incubation Time:24 h
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Result:Reduced levels of IL-1β and IL-18.
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Cell Line:N9 microglial cells
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Concentration:10 μM/L
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Incubation Time:24 h
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Result:Restored migration levels.
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Cell Line:MDA-MB-231 cells
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Concentration:0, 2.5, 5 ,10, 20 μM
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Incubation Time:16 h
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Result:Upregulated the protein levels of Nrf2 and γGCS.
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Cell Line:H2O2-induced EA.hy926 cells
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Concentration:2, 5 μM
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Incubation Time:24 h
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Result:Decreased apoptosis cells.
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Cell Line:EA.hy926 cells
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Concentration:2, 5 μM
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Incubation Time:12 h
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Result:Activated the nuclear translocation of Nrf2 protein.
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Cell Line:EA.hy926 cells
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Concentration:1, 2, 5 μM
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Incubation Time:2, 4, 6, 8, 12, 16 h
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Result:Activated the nuclear translocation of Nrf2 protein.
Increased protein levels of Nrf2, NQO1, and γGCS, continued to increase Nrf2 protein levels over the 24 hours tested.
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Cell Line:EA.hy926 cells
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Concentration:1, 2, 5 μM
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Incubation Time:24 h
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Result:Did not affect mRNA level of Nrf2, but upregulated the levels of NQO1 and GCLM.
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Cell Line:H2O2-induced EA.hy926 cells
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Concentration:2, 5 μM
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Incubation Time:12 h
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Result:Reduce MMP levels.
Reduced cytochrome C protein levels in the cytoplasm and mitochondria.
Inhibited tAIF production and blocks AIF translocation from mitochondria to the nucleus.
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Cell Line:H2O2-induced EA.hy926 cells
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Concentration:1, 2, 5 μM
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Incubation Time:12 h
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Result:Reduced cytochrome C protein levels in the cytoplasm and mitochondria.
Inhibited tAIF production and blocks AIF translocation from mitochondria to the nucleus.
Inhibited H2O2-induced activations of cleaved caspases -3, -9 and PARP.
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Cell Line:MCF-7, HeLa, and HT-29 cells
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Concentration:500 μg/mL
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Incubation Time:24 h
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Result:Increased the green fluorescence of DCF, increased the fluorescence of Rh123 fluorescence.
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Cell Line:MCF-7, HeLa, and HT-29 cells
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Concentration:500 μg/mL
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Incubation Time:24 h
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Result:Increased the subG1 peaks.
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Cell Line:MCF-7, HeLa, and HT-29 cells
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Concentration:100 μg/mL
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Incubation Time:24 h
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Result:Increased the expressions of p53, caspase 3, caspase 9, bax, and SOD1 genes.
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Cell Line:PC3 cells
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Concentration:PC3 cells
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Incubation Time:72 h
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Result:Induced apoptosis, reduced cell density and disrupted cell morphology, enhanced the efficacy of Docetaxel (HY-B0011) leading to higher apoptosis rates.
Increased the mRNA expression of BAX, CASP3, CASP8, and CYCS, upregulated TP53, along with enhanced expression of caspases in combination with Docetaxel.
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Cell Line:PC3 cells
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Concentration:100 μM
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Incubation Time:24 h
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Result:Reduced migration, reduced TWIST, SNAIL, and ZEB1 mRNA expression levels by 59%, 68%, and 42%, respectively, compared to untreated cells.
Homoeriodictyol (50-μg/kg, i.p., once) exhibits antinociceptive activity through both central and peripheral pathways in swiss albino mice model[5].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Aβ25-35-induced AD kunming mice (Male, 7 weeks old) model[1]
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Dosage:10 mg/kg/d
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Administration:p.o., 4 weeks
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Result:Improved memory impairment and cognitive dysfunction, alleviated hippocampal neuronal damage.
Decreased the levels of Aβ1-40, Aβ1-42, and p-Tau in the hippocampus.
Reduced oxidative stress in the brain, reduced the levels of ROS, apoptosis and MDA, increased the levels of GSH-Px and SOD.
Resulted in increased numbers of NK cells, Th cells, Tc cells, and DCs, and decreased numbers of MDSCs and Treg cells in peripheral blood and splenic tissues.
Had excellent anti-inflammatory effects, reduced the protein expression levels of NLRP3, Caspase-1, and ASC, and the inflammatory factors IL-18 and IL-1β in brain tissue.
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Animal Model:Swiss albino mice (adult male, 24-28 g) model[5]
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Dosage:50, 100, 150, and 200 μg/kg
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Administration:i.p., once
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Result:Lowered the writhing responses to acetic acid, showed a dose-responsive relation analgesic effect.
Increased the time respond to thermal pain on a hot plate.
Decreased the licking times in both the early and late phases after formalin injection, alleviated pain in the formalin test.
Lessened licking time after Capsaicin (HY-10448) injection, indicating a dose-responsive effect.
Reduced paw licking times, mitigated glutamate-induced pain responses.
| NCT Number | Sponsor | Condition | Start Date |
Phase
|
|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
Chemical Information
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No. CAS 446-71-9
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Appearance Solid
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Peso molecular 302.28
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Fòrmula C16H14O6
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Color White to off-white
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SMILES
O=C1C[C@@H](C2=CC=C(O)C(OC)=C2)OC3=CC(O)=CC(O)=C13
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Structure Classification
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Initial Source
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvente y solubilidad
DMSO : 50 mg/mL (165.41 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 1.25 mg/mL (4.14 mM); Clear solution
This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 1.25 mg/mL (4.14 mM); Clear solution
This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureza y Documentación
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Ficha de datos (292 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Instrucciones de manejo (2659 KB)
Referencias
[1]. Zhang M, et al. Hesperidin alleviated dendritic spines through inhibiting ferritinophagy via HERC2-NCOA4 ubiquitination in CUMS mice. Phytomedicine. 2024 Dec;135:156132. [Content Brief]
[2]. Guo P, et al. Eriodictyol and Homoeriodictyol Improve Memory Impairment in Aβ25-35-Induced Mice by Inhibiting the NLRP3 Inflammasome. Molecules. 2022 Apr 12;27(8):2488. [Content Brief]
[3]. Shen T, et al. Homoeriodictyol protects human endothelial cells against oxidative insults through activation of Nrf2 and inhibition of mitochondrial dysfunction. Vascul Pharmacol. 2018 Oct;109:72-82. [Content Brief]
[6]. Güvenç A, et al. HOMOERIODICTYOL INHIBITS SURVIVAL AND MIGRATION OF ANDROGEN-RESISTANT PROSTATE CANCER CELLS IN VITRO. Exp Oncol. 2025 Jul 11;47(1):34-43. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.3082 mL | 16.5410 mL | 33.0819 mL | 82.7048 mL |
| 5 mM | 0.6616 mL | 3.3082 mL | 6.6164 mL | 16.5410 mL | |
| 10 mM | 0.3308 mL | 1.6541 mL | 3.3082 mL | 8.2705 mL | |
| 15 mM | 0.2205 mL | 1.1027 mL | 2.2055 mL | 5.5137 mL | |
| 20 mM | 0.1654 mL | 0.8270 mL | 1.6541 mL | 4.1352 mL | |
| 25 mM | 0.1323 mL | 0.6616 mL | 1.3233 mL | 3.3082 mL | |
| 30 mM | 0.1103 mL | 0.5514 mL | 1.1027 mL | 2.7568 mL | |
| 40 mM | 0.0827 mL | 0.4135 mL | 0.8270 mL | 2.0676 mL | |
| 50 mM | 0.0662 mL | 0.3308 mL | 0.6616 mL | 1.6541 mL | |
| 60 mM | 0.0551 mL | 0.2757 mL | 0.5514 mL | 1.3784 mL | |
| 80 mM | 0.0414 mL | 0.2068 mL | 0.4135 mL | 1.0338 mL | |
| 100 mM | 0.0331 mL | 0.1654 mL | 0.3308 mL | 0.8270 mL |
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