RNF8 - ring finger protein 8 Gene

Also Known as hRNF8

Species: Homo sapiens

Gene Type: protein coding

Gene ID: 9025

About RNF8

Cytogenetic location: 6p21.2 Genomic coordinates (GRCh38): 6:37,353,983-37,394,734 (from NCBI)

This gene has 8 transcripts (splice variants) and 192 orthologues. Ubiquitous expression in testis (RPKM 6.4), brain (RPKM 2.8) and 24 other tissues.

Summary

The protein encoded by this gene contains a RING finger motif and an FHA domain. This protein has been shown to interact with several class II ubiquitin-conjugating Enzymes (E2), including UBE2E1/UBCH6, UBE2E2, and UBE2E3, and may act as an ubiquitin Ligase (E3) in the ubiquitination of certain nuclear proteins. This protein is also known to play a role in the DNA damage response and depletion of this protein causes cell growth inhibition and cell cycle arrest. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2012]

RNF8 Products (2)

mRNA	Protein	Name
NM_003958.4	NP_003949.1	E3 ubiquitin-protein ligase RNF8 isoform 1
NM_183078.3	NP_898901.1	E3 ubiquitin-protein ligase RNF8 isoform 2

Gene Ontology

Molecular Function
Biological Process
Cellular Component

Molecular Function GO Annotation	Evidence	References	Source
enables chromatin binding	IDA IDA: Inferred from direct assay	18001824	GOA
enables histone binding	IDA IDA: Inferred from direct assay	18001824	GOA
enables identical protein binding	IPI IPI: Inferred from physical interaction	22980979	GOA
enables protein binding	IPI IPI: Inferred from physical interaction	16189514	GOA
enables protein homodimerization activity	IDA IDA: Inferred from direct assay	22980979	GOA
enables ubiquitin protein ligase activity	IDA IDA: Inferred from direct assay	18001824	GOA
enables ubiquitin protein ligase binding	IPI IPI: Inferred from physical interaction	22266820	GOA
enables zinc ion binding	IDA IDA: Inferred from direct assay	22980979	GOA

Biological Process GO Annotation	Evidence	References	Source
involved in DNA damage response	IDA IDA: Inferred from direct assay	20550933	GOA
involved in DNA repair-dependent chromatin remodeling	IDA IDA: Inferred from direct assay	18001824	GOA
involved in double-strand break repair	IDA IDA: Inferred from direct assay	18001824	GOA
involved in epigenetic regulation of gene expression	IMP IMP: Inferred from mutant phenotype	20550933	GOA
involved in negative regulation of transcription elongation by RNA polymerase II	IMP IMP: Inferred from mutant phenotype	20550933	GOA
involved in positive regulation of DNA repair	IDA IDA: Inferred from direct assay	18001824	GOA
involved in positive regulation of double-strand break repair via homologous recombination	IDA IDA: Inferred from direct assay	23115235	GOA
involved in protein K48-linked ubiquitination	IDA IDA: Inferred from direct assay	21911360	GOA
involved in protein K6-linked ubiquitination	IDA IDA: Inferred from direct assay	23115235	GOA
involved in protein K63-linked ubiquitination	IDA IDA: Inferred from direct assay	21911360	GOA
involved in protein autoubiquitination	IDA IDA: Inferred from direct assay	22266820	GOA
involved in response to ionizing radiation	IDA IDA: Inferred from direct assay	18001824	GOA
involved in ubiquitin-dependent protein catabolic process	IDA IDA: Inferred from direct assay	22266820	GOA

Cellular Component GO Annotation	Evidence	References	Source
located in nucleus	IDA IDA: Inferred from direct assay	18001824	GOA
located in site of double-strand break	IDA IDA: Inferred from direct assay	22266820	GOA
part of ubiquitin ligase complex	IDA IDA: Inferred from direct assay	18001824	GOA

EXP: Inferred from Experiment IDA: Inferred from direct assay IPI: Inferred from physical interaction IMP: Inferred from mutant phenotype IGI: Inferred from genetic interaction IEP: Inferred from expression pattern

RNF8 Protein Structure

FHA

zf-RING_2

0
100
200
300
400
485 a.a.

Protein Preferred Names	Protein Names
E3 ubiquitin-protein ligase RNF8	C3HC4-type zinc finger protein

RNF8 Protein-protein interaction Information

Type	Protein Name	Protein ID	Interactor	Interactor Species	Interactor ID	Detection Method	References
Intra	RNF8	O76064	KRTAP10-8	Homo sapiens	P60410	Y2H Prey Pooling	32296183
Intra	RNF8	O76064	KRTAP10-8	Homo sapiens	P60410	Validated Y2H	32296183
Intra	RNF8	O76064	KRTAP10-8	Homo sapiens	P60410	Y2H Array	32296183
Intra	RNF8	O76064	HOMEZ	Homo sapiens	Q8IX15-3	Validated Y2H	25416956
Intra	RNF8	O76064	FAM9B	Homo sapiens	Q8IZU0	Validated Y2H	32296183
Intra	RNF8	O76064	KIF24	Homo sapiens	Q5T7B8-2	Y2H Array	32296183
Intra	RNF8	O76064	KIF24	Homo sapiens	Q5T7B8-2	Y2H Prey Pooling	32296183
Intra	RNF8	O76064	KRTAP9-2	Homo sapiens	Q9BYQ4	Validated Y2H	32296183
Intra	RNF8	O76064	PGRMC2	Homo sapiens	O15173	Y2H Array	32296183
Intra	RNF8	O76064	PGRMC2	Homo sapiens	O15173	Y2H Prey Pooling	32296183
Intra	RNF8	O76064	TOMM20L	Homo sapiens	Q6UXN7	Y2H Prey Pooling	32296183
Intra	RNF8	O76064	TOMM20L	Homo sapiens	Q6UXN7	Validated Y2H	32296183
Intra	RNF8	O76064	TOMM20L	Homo sapiens	Q6UXN7	Y2H Array	32296183
Intra	RNF8	O76064	KRTAP9-8	Homo sapiens	Q9BYQ0	Validated Y2H	32296183
Intra	RNF8	O76064	RBFOX2	Homo sapiens	O43251-10	Y2H Array	32296183
Intra	RNF8	O76064	RBFOX2	Homo sapiens	O43251-10	Y2H Prey Pooling	32296183
Intra	RNF8	O76064	ZBTB10	Homo sapiens	Q96DT7-3	Validated Y2H	32296183
Intra	RNF8	O76064	ZBTB10	Homo sapiens	Q96DT7-3	Y2H Array	32296183
Intra	RNF8	O76064	ZBTB10	Homo sapiens	Q96DT7-3	Y2H Prey Pooling	32296183
Intra	RNF8	O76064	CCDC14	Homo sapiens	Q49A88-3	Validated Y2H	32296183
Intra	RNF8	O76064	EPN2	Homo sapiens	O95208-2	Y2H Array	32296183
Intra	RNF8	O76064	EPN2	Homo sapiens	O95208-2	Y2H Prey Pooling	32296183
Intra	RNF8	O76064	EPN2	Homo sapiens	O95208-2	Validated Y2H	32296183
Intra	RNF8	O76064	CEP85	Homo sapiens	Q6P2H3-3	Y2H Array	32296183
Intra	RNF8	O76064	CEP85	Homo sapiens	Q6P2H3-3	Y2H Prey Pooling	32296183
Intra	RNF8	O76064	CEP85	Homo sapiens	Q6P2H3-3	Validated Y2H	32296183
Intra	RNF8	O76064	EPN3	Homo sapiens	I6L9I8	Y2H Prey Pooling	32296183
Intra	RNF8	O76064	EPN3	Homo sapiens	I6L9I8	Y2H Array	32296183
Intra	RNF8	O76064	SCN3B	Homo sapiens	Q9NY72	Validated Y2H	32296183
Intra	RNF8	O76064	SCN3B	Homo sapiens	Q9NY72	Y2H Array	32296183
Intra	RNF8	O76064	SCN3B	Homo sapiens	Q9NY72	Y2H Prey Pooling	32296183
Intra	RNF8	O76064	VRK1	Homo sapiens	Q99986	Anti Tag CoIP	26869104
Intra	RNF8	O76064	ZNF346	Homo sapiens	Q9UL40	Y2H Prey Pooling	32296183
Intra	RNF8	O76064	ZNF346	Homo sapiens	Q9UL40	Validated Y2H	32296183
Intra	RNF8	O76064	ZNF346	Homo sapiens	Q9UL40	Y2H Array	32296183
Intra	RNF8	O76064	KANK2	Homo sapiens	Q63ZY3	Y2H Prey Pooling	32296183
Intra	RNF8	O76064	KANK2	Homo sapiens	Q63ZY3	Validated Y2H	32296183
Intra	RNF8	O76064	KANK2	Homo sapiens	Q63ZY3	Y2H Array	32296183
Intra	RNF8	O76064	PCYT1A	Homo sapiens	P49585	Validated Y2H	32296183
Intra	RNF8	O76064	PCYT1A	Homo sapiens	P49585	Y2H Array	32296183
Intra	RNF8	O76064	PCYT1A	Homo sapiens	P49585	Y2H Prey Pooling	32296183
Intra	RNF8	O76064	LMO4	Homo sapiens	P61968	Validated Y2H	32296183
Intra	RNF8	O76064	PNMA2	Homo sapiens	Q9UL42	Validated Y2H	32296183
Intra	RNF8	O76064	PNMA2	Homo sapiens	Q9UL42	Y2H Array	32296183
Intra	RNF8	O76064	PNMA2	Homo sapiens	Q9UL42	Y2H	21516116
Intra	RNF8	O76064	PNMA2	Homo sapiens	Q9UL42	Y2H Prey Pooling	32296183
Intra	RNF8	O76064	DNM2	Homo sapiens	P50570	Y2H Prey Pooling	32296183
Intra	RNF8	O76064	DNM2	Homo sapiens	P50570	Validated Y2H	32296183
Intra	RNF8	O76064	DNM2	Homo sapiens	P50570	Y2H Array	32296183
Intra	RNF8	O76064	UBE2D2	Homo sapiens	P62837	Y2H Array	19690564
Intra	RNF8	O76064	UBE2D2	Homo sapiens	P62837	Validated Y2H	32296183
Intra	RNF8	O76064	VCP	Homo sapiens	P55072	Y2H Prey Pooling	25416956
Intra	RNF8	O76064	VCP	Homo sapiens	P55072	Validated Y2H	25416956
Intra	RNF8	O76064	TNIP1	Homo sapiens	Q15025	Y2H Array	32296183
Intra	RNF8	O76064	TNIP1	Homo sapiens	Q15025	Y2H Prey Pooling	32296183
Intra	RNF8	O76064	TNIP1	Homo sapiens	Q15025	Validated Y2H	32296183
Intra	RNF8	O76064	PSMB1	Homo sapiens	P20618	Validated Y2H	32296183
Intra	RNF8	O76064	MAD1L1	Homo sapiens	Q9Y6D9	Validated Y2H	32296183
Intra	RNF8	O76064	WASL	Homo sapiens	O00401	Y2H Prey Pooling	32296183
Intra	RNF8	O76064	MAD1L1	Homo sapiens	Q9Y6D9	Y2H Array	25416956
Intra	RNF8	O76064	SEPTIN3	Homo sapiens	Q9UH03	Y2H Prey Pooling	25416956
Intra	RNF8	O76064	MAD1L1	Homo sapiens	Q9Y6D9	Y2H Array	32296183
Intra	RNF8	O76064	SEPTIN3	Homo sapiens	Q9UH03	Y2H Prey Pooling	32296183
Intra	RNF8	O76064	MAD1L1	Homo sapiens	Q9Y6D9	Y2H Prey Pooling	25416956
Intra	RNF8	O76064	WASL	Homo sapiens	O00401	Y2H	21516116
Intra	RNF8	O76064	SEPTIN3	Homo sapiens	Q9UH03	Validated Y2H	25416956
Intra	RNF8	O76064	WASL	Homo sapiens	O00401	Y2H Array	32296183
Intra	RNF8	O76064	MAD1L1	Homo sapiens	Q9Y6D9	Validated Y2H	25416956
Intra	RNF8	O76064	MAD1L1	Homo sapiens	Q9Y6D9	Y2H Prey Pooling	32296183
Intra	RNF8	O76064	GPRASP2	Homo sapiens	Q96D09	Y2H Prey Pooling	32296183
Intra	RNF8	O76064	GPRASP2	Homo sapiens	Q96D09	Y2H Array	32296183
Intra	RNF8	O76064	GPRASP2	Homo sapiens	Q96D09	Validated Y2H	32296183
Intra	RNF8	O76064	MDC1	Homo sapiens	Q14676	Anti Bait CoIP	18001824
Intra	RNF8	O76064	MDC1	Homo sapiens	Q14676	Pull Down	18001825
Intra	RNF8	O76064	FSD2	Homo sapiens	A1L4K1	Y2H Array	32296183
Intra	RNF8	O76064	FSD2	Homo sapiens	A1L4K1	Y2H Prey Pooling	32296183
Intra	RNF8	O76064	DNTTIP2	Homo sapiens	Q5QJE6	Validated Y2H	32296183
Intra	RNF8	O76064	SH3GL3	Homo sapiens	Q8IVP1	Validated Y2H	32296183
Intra	RNF8	O76064	SH3GL3	Homo sapiens	Q8IVP1	Y2H Array	32296183
Intra	RNF8	O76064	SH3GL3	Homo sapiens	Q8IVP1	Y2H Prey Pooling	32296183
Intra	RNF8	O76064	WASHC3	Homo sapiens	Q9Y3C0	Y2H Prey Pooling	32296183
Intra	RNF8	O76064	WASHC3	Homo sapiens	Q9Y3C0	Validated Y2H	32296183
Intra	RNF8	O76064	WASHC3	Homo sapiens	Q9Y3C0	Y2H Array	32296183
Intra	RNF8	O76064	STX11	Homo sapiens	O75558	Y2H Prey Pooling	32296183
Intra	RNF8	O76064	STX11	Homo sapiens	O75558	Y2H Array	32296183
Intra	RNF8	O76064	CCDC50	Homo sapiens	Q8IVM0	Validated Y2H	32296183
Intra	RNF8	O76064	CCDC50	Homo sapiens	Q8IVM0	Y2H Array	32296183
Intra	RNF8	O76064	CCDC50	Homo sapiens	Q8IVM0	Y2H Prey Pooling	32296183
Intra	RNF8	O76064	PPCDC	Homo sapiens	Q96CD2	Y2H Prey Pooling	32296183
Intra	RNF8	O76064	PPCDC	Homo sapiens	Q96CD2	Validated Y2H	32296183
Intra	RNF8	O76064	PPCDC	Homo sapiens	Q96CD2	Y2H Array	32296183
Intra	RNF8	O76064	FAM118B	Homo sapiens	Q9BPY3	Y2H Prey Pooling	32296183
Intra	RNF8	O76064	FAM118B	Homo sapiens	Q9BPY3	Validated Y2H	32296183
Intra	RNF8	O76064	FAM118B	Homo sapiens	Q9BPY3	Y2H Array	32296183
Intra	RNF8	O76064	SEPTIN3	Homo sapiens	Q9UH03	Validated Y2H	32296183
Intra	RNF8	O76064	SEPTIN3	Homo sapiens	Q9UH03	Y2H Array	32296183
Intra	RNF8	O76064	C2CD6	Homo sapiens	Q53TS8	Validated Y2H	32296183
Intra	RNF8	O76064	DDIT4L	Homo sapiens	Q96D03	Y2H Array	32296183
Intra	RNF8	O76064	DDIT4L	Homo sapiens	Q96D03	Y2H Prey Pooling	32296183
Intra	RNF8	O76064	DDIT4L	Homo sapiens	Q96D03	Validated Y2H	32296183
Intra	RNF8	O76064	ZNF496	Homo sapiens	Q96IT1	Validated Y2H	32296183
Intra	RNF8	O76064	ZNF496	Homo sapiens	Q96IT1	Y2H Array	32296183
Intra	RNF8	O76064	ZNF496	Homo sapiens	Q96IT1	Y2H Prey Pooling	32296183
Intra	RNF8	O76064	CEP44	Homo sapiens	Q9C0F1	Y2H Pooling	16189514
Intra	RNF8	O76064	CEP44	Homo sapiens	Q9C0F1	Y2H Prey Pooling	32296183
Intra	RNF8	O76064	CEP44	Homo sapiens	Q9C0F1	Validated Y2H	32296183
Intra	RNF8	O76064	CEP44	Homo sapiens	Q9C0F1	Y2H Array	32296183
Intra	RNF8	O76064	CLK3	Homo sapiens	P49761	Y2H Array	32296183
Intra	RNF8	O76064	CLK3	Homo sapiens	P49761	Y2H Prey Pooling	32296183
Intra	RNF8	O76064	CLK3	Homo sapiens	P49761	Validated Y2H	32296183
Intra	RNF8	O76064	RBFOX2	Homo sapiens	O43251	Validated Y2H	25416956
Intra	RNF8	O76064	RBFOX2	Homo sapiens	O43251	Y2H Prey Pooling	25416956
Intra	RNF8	O76064	AMOTL2	Homo sapiens	Q9Y2J4	Validated Y2H	32296183
Intra	RNF8	O76064	AMOTL2	Homo sapiens	Q9Y2J4	Y2H Array	32296183
Intra	RNF8	O76064	AMOTL2	Homo sapiens	Q9Y2J4	Y2H Prey Pooling	32296183
Intra	RNF8	O76064	CALCOCO1	Homo sapiens	Q9P1Z2	Y2H Array	32296183
Intra	RNF8	O76064	CALCOCO1	Homo sapiens	Q9P1Z2	Y2H Prey Pooling	32296183
Intra	RNF8	O76064	CALCOCO1	Homo sapiens	Q9P1Z2	Validated Y2H	32296183
Intra	RNF8	O76064	CLK2	Homo sapiens	P49760	Validated Y2H	32296183
Intra	RNF8	O76064	PICK1	Homo sapiens	Q9NRD5	Y2H Prey Pooling	32296183
Intra	RNF8	O76064	PICK1	Homo sapiens	Q9NRD5	Validated Y2H	32296183
Intra	RNF8	O76064	PICK1	Homo sapiens	Q9NRD5	Y2H Array	32296183
Intra	RNF8	O76064	TMEM79	Homo sapiens	Q9BSE2	Y2H Prey Pooling	32296183
Intra	RNF8	O76064	TMEM79	Homo sapiens	Q9BSE2	Validated Y2H	32296183
Intra	RNF8	O76064	TMEM79	Homo sapiens	Q9BSE2	Y2H Array	25416956
Intra	RNF8	O76064	TMEM79	Homo sapiens	Q9BSE2	Y2H Array	32296183
Intra	RNF8	O76064	TMEM79	Homo sapiens	Q9BSE2	Y2H Array	31515488
Intra	RNF8	O76064	TMEM79	Homo sapiens	Q9BSE2	Y2H Prey Pooling	25416956
Intra	RNF8	O76064	MAPK1	Homo sapiens	P28482	Validated Y2H	32296183

Cross: Cross-species interaction Intra: Intraspecies interaction

RNF8 Antibodies

Cat. No.	Product Name	Application	Reactivity
HY-P81599	RNF8 Antibody (YA1344)	WB	Human, Rat

Related Diseases

Diseases	Alias
Riddle Syndrome	RIDL Radiosensitivity-Immunodeficiency-Dysmorphic Features-Learning Difficulties Syndrome Rnf168 Deficiency Radiosensitivity, Immunodeficiency, Dysmorphic Facial Features, And Learning Difficulties Radiosensitivity, Immunodeficiency, Dysmorphic Features, And Learning Difficulties
Dyskeratosis Congenita, Autosomal Recessive 3	DKCB3 Autosomal Recessive Dyskeratosis Congenita 3 Dyskeratosis Congenita, Autosomal Recessive, 3 Dyskeratosis Congenita, Autosomal Recessive, Type 3
Cornelia De Lange Syndrome	De Lange Syndrome Brachmann De Lange Syndrome Brachmann-De Lange Syndrome Cdls Bdls Typus Degenerativus Amstelodamensis
Ataxia-Telangiectasia	Ataxia Telangiectasia Louis-Bar Syndrome AT At1 Ataxia-Telangiectasia Syndrome Ataxia - Telangiectasia Variant Boder-Sedgwick Syndrome Louis Bar Syndrome Cerebello-Oculocutaneous Telangiectasia Immunodeficiency With Ataxia Telangiectasia A-T Ataxia Telangiectasia Syndrome Atm Telangiectasia, Cerebello-Oculocutaneous Ataxia-Telangiectasia Variant
Angelman Syndrome	AS Happy Puppet Syndrome Happy Puppet Syndrome, Formerly Puppetlike Syndrome
Xeroderma Pigmentosum, Variant Type	Xeroderma Pigmentosum XPV Xeroderma Pigmentosum Variant Type Xeroderma Pigmentosum With Normal Dna Repair Rates Photosensitivity With Defective Dna Synthesis Xp De Sanctis-Cacchione Syndrome Desanctis-Cacchione Syndrome Xeroderma Pigmentosa Xerodermic Idiocy Xeroderma Pigmentosum Variant Xp - [Xeroderma Pigmentosum] Atrophoderma Pigmentosum
Fanconi Anemia, Complementation Group A	Fanconi Anemia Fanconi Pancytopenia Fanconi Anemia Complementation Group A FANCA Fa Fanconi Panmyelopathy Fanconi'S Anemia Fanconi Anaemia Fanconi'S Anaemia Fanconi Hypoplastic Anemia Estren-Dameshek Variant Of Fanconi Anemia Estren-Dameshek Variant Of Fanconi Pancytopenia Fanconi Anemia Estren-Dameshek Variant Fanconis Anemia
Hereditary Breast Ovarian Cancer Syndrome	Hereditary Breast And Ovarian Cancer Syndrome Brca1- And Brca2-Associated Hereditary Breast And Ovarian Cancer Breast And/Or Ovarian Cancer Breast And Ovarian Cancer Syndrome Hboc Syndrome Hereditary Breast And Ovarian Cancer Brca1- Brca2-Associated Hboc

Diseases	Alias
Waldenstroem'S Macroglobulinemia	Waldenstroem'S Macroglobulinemia Waldenstroem'S Macroglobulinemia Macroglobulinemia Of Waldenstrom Lymphoplasmacytic Lymphoma With Igm Gammopathy Lymphoplasmacytic Lymphoma Waldenstroem'S Macroglobulinemia Waldenstroem'S Macroglobulinemia Macroglobulinemia Of Waldenstrom Lymphoplasmacytic Lymphoma With Igm Gammopathy Lymphoplasmacytic Lymphoma

Related Agents

Pre-clinical Phase

Pre-clinical Phase

Bioactive Molecules (3)

Cat. No.	Product Name	Effect	Purity	Rare Diseases
HY-RS12097	RNF8 Human Pre-designed siRNA Set A	Pre-designed Set	/	Unkown
HY-RS18369	Rnf8 Mouse Pre-designed siRNA Set A	Pre-designed Set	/	Unkown
HY-RS24848	Rnf8 Rat Pre-designed siRNA Set A	Pre-designed Set	/	Unkown

Orthologs Information

Species	Symbol	Source	ID
Rattus norvegicus	RNF8	RGD	RGD:1308035
Mus musculus	RNF8	MGD	MGI:1929069
Felis catus	RNF8	VGNC	VGNC:102507
Macaca mulatta	RNF8	VGNC	VGNC:76708
Canis familiaris	RNF8	VGNC	VGNC:45676
Bos taurus	RNF8	VGNC	VGNC:34073
Others	RNF8	NCBI