Cat. No.: HY-N0029

Forsythoside B

8 Cited Publications

Customer Review

Based on 8 publication(s) in Google Scholar

Forsythoside B is a phenylethanoid glycoside isolated from Forsythia suspensa (Thunb.) Vahl, a Chinese folk medicinal plant for treating inflammatory diseases and promoting blood circulation. Forsythoside B could inhibit TNF-alpha, IL-6, IκB and modulate NF-κB.

For research use only. We do not sell to patients.

Purity: 99.90%
CAS No.: 81525-13-5
Formula: C₃₄H₄₄O₁₉
Molecular Weight:756.70

Storage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

Forsythoside B Chemical Structure

CAS No.: 81525-13-5

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Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
- In Vivo
Chemical Information
Publications (8)
Solvent & Solubility
- In Vitro
- In Vivo
Protocol
Purity & Documentation
References
Help & FAQs
Bioz

Biological Activity

Description

IC₅₀ & Target^[1]

NF-κB

All TNF Receptor Isoforms :

All NF-κB Isoforms :

In Vitro

Forsythoside B concentration-dependently down-regulates the levels of TNF-α, IL-6 and high-mobility group-box 1 protein (HMGB1) in lipopolysaccharide (LPS)-stimulated RAW264.7 cells, inhibits the IκB kinase (IKK) pathway and modulated nuclear factor (NF)- κB^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Intravenous injection of Forsythoside B (HY-N0029) alone or plus Imipenem (HY-B1369A) reduces serum levels of TNF-α, IL-6, HMGB1, triggering receptor expressed on myeloid cells (TREM-1) and endotoxin, while the serum level of IL-10 is up-regulated and myeloperoxidase (MPO) in lung, liver and small intestine is reduced^[1]. Forsythoside B at doses higher than 8 mg/kg produces a significant neuroprotective potential in cerebral ischemia and reperfusion rats. Forsythoside B (20 mg/kg) demonstrates significant neuroprotective activity even after delayed administration at 1 h, 3 h and 5 h after cerebral ischemia and reperfusion. Forsythoside B 20 mg/kg attenuates histopathological damage as demonstrated by smaller brain infarct size and brain edema, decreased cerebral Evans blue extravasation and myeloperoxidase activity, inhibited cerebral phosphor-IκB-α and NF-κB expression^[2]. Forsythoside B shows a significant recovery in myocardial function with improvement of LVSP and +/-dp/dt(max). The myocardial infarct volume, serum levels of Tn-T, TNF-alpha and IL-6, content of MDA and MPO activity in myocardial tissue are all reduced, protein expression of HMGB1, phosphor-I kappaB-alpha and phosphor-NF-kappaB are down-regulated, while attenuate the decrease of SOD and GPx activities^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 81525-13-5
Appearance Solid
Molecular Weight 756.70
Formula C₃₄H₄₄O₁₉
Color White to light yellow
SMILES

OC1=C(O)C=C(/C=C/C(O[C@H]2[C@H](O[C@]3([H])O[C@@H](C)[C@H](O)[C@@H](O)[C@H]3O)[C@@H](O)[C@H](OCCC4=CC=C(O)C(O)=C4)O[C@@H]2CO[C@H]5[C@H](O)[C@](CO)(O)CO5)=O)C=C1
Structure Classification
- Phenylpropanoids
- Simple Phenylpropanols
- Phenols
- Polyphenols
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Publications (8)

All In Vivo Efficacy StudyELISAIFWB

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Journal Impact Factor

Cell Death Differ

CSTF2-impeded innate αβ T cell infiltration and activation exacerbate immune evasion of pancreatic cancer. [Abstract]

2025 May;32(5):973-988. PMID: 39972059

Forsythoside B purchased from MedChemExpress. Usage Cited in: Cell Death Differ. 2025 May;32(5):973-988. [Abstract]

Representative luminescence images of PDAC mice treated with Forsythoside B (FTS·B) (200 mg/kg; i.v.; once every 3 d).

Forsythoside B purchased from MedChemExpress. Usage Cited in: Cell Death Differ. 2025 May;32(5):973-988. [Abstract]

Kaplan-Meier curves indicated survival of PDAC mice treated with Forsythoside B (FTS·B) (200 mg/kg; i.v.; once every 3 d).

Forsythoside B purchased from MedChemExpress. Usage Cited in: Cell Death Differ. 2025 May;32(5):973-988. [Abstract]

Forsythoside B (FTS·B) (200 mg/kg; i.v.; once every 3 d) increased the TIF of CSTF2^+/+ tumors in C57BL/6 mouse PDAC models.

Forsythoside B purchased from MedChemExpress. Usage Cited in: Cell Death Differ. 2025 May;32(5):973-988. [Abstract]

Forsythoside B (FTS·B) (200 mg/kg; i.v.; once every 3 d) significantly inhibited Cstf2^+/+ tumor growth of PDAC mice in a CD8⁺ T and NK cell-dependent manner.

Forsythoside B purchased from MedChemExpress. Usage Cited in: Cell Death Differ. 2025 May;32(5):973-988. [Abstract]

Forsythoside B (FTS·B) (200 mg/kg; i.v.; once every 3 d) yielded great intratumoral infiltration of iαβT cells in PDAC mice.
Free Radic Biol Med

Virtual screening identified natural Keap1-Nrf2 PPI inhibitor alleviates inflammatory osteoporosis through Nrf2-mir214-Traf3 axis. [Abstract]

2021 Aug 1:171:365-378. PMID: 34000381
J Ethnopharmacol

Borneol exerts its antipruritic effects by inhibiting TRPA1 and activating TRPM8. [Abstract]

2024 Mar 25:322:117581. PMID: 38103845
Int Immunopharmacol

Forsythoside B protects cartilage and subchondral bone in osteoarthritis by regulating the Nrf2/NF - κ B signaling pathway. [Abstract]

2026 Jan 1;168(Pt 1):115762. PMID: 41176908
Neuropharmacology

Forsythoside B suppresses glioblastoma by upregulating the expression of PTPRN. [Abstract]

2025 May 16:110514. PMID: 40383262
J Biochem Mol Toxicol

Forsythoside B alleviates osteoarthritis through the HMGB1/TLR4/NF-κB and Keap1/Nrf2/HO-1 pathways. [Abstract]

2024 Jan;38(1):e23569. PMID: 37943572
Biochem Biophys Res Commun

Anti-inflammatory and analgesic effect of Forsythiaside B on complete Freund's adjuvant-induced inflammatory pain in mice. [Abstract]

2023 Feb 19:645:55-60. PMID: 36680937

Forsythoside B purchased from MedChemExpress. Usage Cited in: Biochem Biophys Res Commun. 2023 Feb 19:645:55-60. [Abstract]

Forsythoside B (FB; 10 mg/kg; i.p; single daily for 3 days; male C57BL/6 mice) reduces the up-regulation of TNF-α and IL-6 in the spinal cord after CFA (50% complete Freund's adjuvant ) injection.
Biochem Biophys Res Commun

Inhibition of the transient receptor potential vanilloid 3 channel attenuates carbon tetrachloride-induced hepatic fibrosis. [Abstract]

2021 Jun 18:558:86-93. PMID: 33906111

Solvent & Solubility

In Vitro:

DMSO : 125 mg/mL (165.19 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.3215 mL

6.6076 mL

13.2153 mL

5 mM

0.2643 mL

1.3215 mL

2.6431 mL

10 mM

0.1322 mL

0.6608 mL

1.3215 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

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Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.08 mg/mL (2.75 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.08 mg/mL (2.75 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.08 mg/mL (2.75 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Protocol

Cell Assay^[1]

Forsythoside B is dissolved in sterile saline solution and added to the medium at various concentrations (from 0.1 to 10 μM) and incubated with LPS stimulated RAW264.7 cells. Cell-free supernatants are collected after Forsythoside B treatment for 24 h. Cell viability is assessed by measuring lactate dehydrogenase (LDH) in the medium^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Administration^[2]

Rats: Forsythoside B is dissolved in sterilized saline. For the dose–response study, forsythoside B at doses of 1.3, 3.2, 8, 20 or 50 mg/kg is administered as an intravenous bolus injection at 15 min after reperfusion. The sham or vehicle-treated rats are injected with saline. Neurological deficits are determined at 23 h after reperfusion followed by brain infarct volume examination^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (285 KB)
SDS (392 KB)
- English - EN (392 KB)
- Français - FR (392 KB)
- Deutsch - DE (392 KB)
- Norwegian - NO (392 KB)
- Español - ES (392 KB)
- Swedish - SV (392 KB)
- Italian - IT (392 KB)
- Portuguese - PT (392 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Jiang WL, et al. Forsythoside B protects against experimental sepsis by modulating inflammatory factors. Phytother Res. 2012 Jul;26(7):981-7. [Content Brief]

[2]. Jiang WL, et al. Neuroprotective efficacy and therapeutic window of Forsythoside B: in a rat model of cerebral ischemia and reperfusion injury. Eur J Pharmacol. 2010 Aug 25;640(1-3):75-81. [Content Brief]

[3]. Jiang WL, et al. Cardioprotection with forsythoside B in rat myocardial ischemia-reperfusion injury: relation to inflammation response. Phytomedicine. 2010 Jul;17(8-9):635-9. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	1.3215 mL	6.6076 mL	13.2153 mL	33.0382 mL
	5 mM	0.2643 mL	1.3215 mL	2.6431 mL	6.6076 mL
	10 mM	0.1322 mL	0.6608 mL	1.3215 mL	3.3038 mL
	15 mM	0.0881 mL	0.4405 mL	0.8810 mL	2.2025 mL
	20 mM	0.0661 mL	0.3304 mL	0.6608 mL	1.6519 mL
	25 mM	0.0529 mL	0.2643 mL	0.5286 mL	1.3215 mL
	30 mM	0.0441 mL	0.2203 mL	0.4405 mL	1.1013 mL
	40 mM	0.0330 mL	0.1652 mL	0.3304 mL	0.8260 mL
	50 mM	0.0264 mL	0.1322 mL	0.2643 mL	0.6608 mL
	60 mM	0.0220 mL	0.1101 mL	0.2203 mL	0.5506 mL
	80 mM	0.0165 mL	0.0826 mL	0.1652 mL	0.4130 mL
	100 mM	0.0132 mL	0.0661 mL	0.1322 mL	0.3304 mL

Keywords

Forsythoside B

8 Cited Publications

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Forsythoside B

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Publications Citing Use of MedChemExpress (MCE) Forsythoside B

All TNF Receptor Isoforms

All NF-κB Isoforms

Biological Activity

Chemical Information

Publications (8)

Solvent & Solubility

Protocol

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Forsythoside B Related Classifications

Cat. No.: HY-N0029