Autophagy inducer 7
Based on 1 Customer Validation
Autophagy inducer 7 (Compound SSA) is an Autophagy and Apoptosis inducer. Autophagy inducer 7 activates autophagy by inhibiting Akt/mTOR signaling and the expression of downstream proteins. Autophagy inducer 7 suppresses DNA synthesis and causes a G0-G1 cell-cycle arrest. Autophagy inducer 7 inhibits tumor cell growth.
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- 純度 : 99.45%
- CAS 番号: 944159-20-0
- 分子式: C24H27FN2OS
- 分子量:410.55
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保管条件:
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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生物活性
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化学情報
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溶剤 & 溶解度
- プロトコル
- 純度とドキュメンテーション
- 参考文献
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Help & FAQs
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生物活性
製品説明
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| BJ | EC50 |
>7.57 μM
Compound: 1; SSA
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Growth inhibition of human BJ cells after 72 hrs by CellTiter-Glo reagent based assay
Growth inhibition of human BJ cells after 72 hrs by CellTiter-Glo reagent based assay
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[PMID: 28935266] |
| HT-29 | CC50 |
0.65 μM
Compound: 1; SSA
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Growth inhibition of human HT-29 cells after 72 hrs by CellTiter-Glo reagent based assay
Growth inhibition of human HT-29 cells after 72 hrs by CellTiter-Glo reagent based assay
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[PMID: 28935266] |
| HT-29 | CC50 |
0.65 μM
Compound: SSA
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Growth inhibition of human HT-29 cells measured after 72 hrs by Celltiter-Glo reagent based assay
Growth inhibition of human HT-29 cells measured after 72 hrs by Celltiter-Glo reagent based assay
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[PMID: 29776741] |
| HT-29 | IC50 |
11.3 μM
Compound: 36
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Antiproliferative activity against human HT-29 cells after 72 hrs by MTT assay
Antiproliferative activity against human HT-29 cells after 72 hrs by MTT assay
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[PMID: 22940705] |
| MDA-MB-231 | CC50 |
2.67 μM
Compound: 1; SSA
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Growth inhibition of human MDA-MB-231 cells after 72 hrs by CellTiter-Glo reagent based assay
Growth inhibition of human MDA-MB-231 cells after 72 hrs by CellTiter-Glo reagent based assay
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[PMID: 28935266] |
| MDA-MB-231 | CC50 |
2.67 μM
Compound: SSA
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Growth inhibition of human MDA-MB-231 cells measured after 96 hrs by Celltiter-Glo reagent based assay
Growth inhibition of human MDA-MB-231 cells measured after 96 hrs by Celltiter-Glo reagent based assay
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[PMID: 29776741] |
| PC-3 | CC50 |
3.12 μM
Compound: 1; SSA
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Growth inhibition of human PC3 cells after 72 hrs by CellTiter-Glo reagent based assay
Growth inhibition of human PC3 cells after 72 hrs by CellTiter-Glo reagent based assay
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[PMID: 28935266] |
| PC-3 | CC50 |
3.12 μM
Compound: SSA
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Growth inhibition of human PC3 cells measured after 72 hrs by Celltiter-Glo reagent based assay
Growth inhibition of human PC3 cells measured after 72 hrs by Celltiter-Glo reagent based assay
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[PMID: 29776741] |
体外実験
Autophagy inducer 7 (72 h) inhibits the growth of human lung adenocarcinoma cell lines, with IC50s of 1.95 μM (HOP-62), 4.72 μM (A549), 3.05μM (H1299)[1].
Autophagy inducer 7 (0-10 μM, 24 h) induces apoptosis in HOP-62, A549, H1299 cells, but not significant as Sulindac sulfide (HY-B1786) and Staurosporine (HY-15141)[1].
Autophagy inducer 7 (0-7.5 μM, 24 h) inhibits DNA synthesis in HOP-62, A549, H1299 cells, and increases the percentage of cells in the G0-G1 phase of the cell cycle[1].
Autophagy inducer 7 (0-7.5 μM, 24 h) induces autophagy and increases autophagic flux by inhibiting Akt/mTOR/p70S6k signaling in A549 cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:HOP-62, A549, H1299 cells
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Concentration:2.5, 5, 7.5, 10 μM
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Incubation Time:24 h
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Result:Induced caspase3/7 activation dose-dependently.
Induced cleaved PARP expression at 10 μM.
Increased Annexin-V surface staining and PI labeling.
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Cell Line:HOP-62, A549, H1299 cells
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Concentration:2.5, 5, 7.5, 10 μM
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Incubation Time:24 h
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Result:Induced dose- and time-dependent cleavage of cytosolic LC3-I to autophagosome-associated LC3-II.
Decreased p62 expression.
Displayed a striking accumulation of vesicle-like structures within the cytoplasm.
Increased both the number and size of acidic vesicles within the cytoplasm.
- RNA extraction experimental
- Annexin V plus membrane-impermeant dye apoptosis staining
- BrdU Incorporation Assay
- Somatic Cell Culture
- CCK-8/WST-8 Cell Proliferation Assay
- Cell Counting-Based Growth Curve Assay
- Cell Viability Determination by MTT Colorimetric Assay
- EdU Incorporation Assay (Click Chemistry-Based DNA Synthesis Measurement)
- Detection of Apoptosis
- TUNEL staining for apoptotic DNA fragmentation
- Protocol for Cell Counting and Cell Density Analysis
- Autophagy
- Apoptosis Solutions
- Lysosome and acidic-vesicle live-cell staining
- MTT Cell Proliferation Assay
- Macroautophagy Solutions
化学情報
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CAS 番号 944159-20-0
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性状 Solid
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分子量 410.55
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分子式 C24H27FN2OS
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Color Light yellow to yellow
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SMILES
CN(C)CCNC(CC1=C(C)/C(C2=CC=C(F)C=C21)=C/C3=CC=C(C=C3)SC)=O
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
溶剤 & 溶解度
体外:
DMSO : 50 mg/mL (121.79 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
純度とドキュメンテーション
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データシート (273 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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取扱説明書 (2659 KB)
参考文献
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.4358 mL | 12.1788 mL | 24.3576 mL | 60.8939 mL |
| 5 mM | 0.4872 mL | 2.4358 mL | 4.8715 mL | 12.1788 mL | |
| 10 mM | 0.2436 mL | 1.2179 mL | 2.4358 mL | 6.0894 mL | |
| 15 mM | 0.1624 mL | 0.8119 mL | 1.6238 mL | 4.0596 mL | |
| 20 mM | 0.1218 mL | 0.6089 mL | 1.2179 mL | 3.0447 mL | |
| 25 mM | 0.0974 mL | 0.4872 mL | 0.9743 mL | 2.4358 mL | |
| 30 mM | 0.0812 mL | 0.4060 mL | 0.8119 mL | 2.0298 mL | |
| 40 mM | 0.0609 mL | 0.3045 mL | 0.6089 mL | 1.5223 mL | |
| 50 mM | 0.0487 mL | 0.2436 mL | 0.4872 mL | 1.2179 mL | |
| 60 mM | 0.0406 mL | 0.2030 mL | 0.4060 mL | 1.0149 mL | |
| 80 mM | 0.0304 mL | 0.1522 mL | 0.3045 mL | 0.7612 mL | |
| 100 mM | 0.0244 mL | 0.1218 mL | 0.2436 mL | 0.6089 mL |
Autophagy inducer 7 関連分類
HY-171047 関連分類
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