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製品番号: HY-N21815

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Phenoxan

Phenoxan is an inhibitor of cytochrome b and NADH: ubiquinone oxidoreductase. It exerts anti-HIV, antifungal, and cytotoxic effects by inhibiting the electron transport chain, the activity of HIV-1 Reverse Transcriptase, and viral replication. Phenoxan can be used in studies of HIV-1 infection and fungal infections.

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CAS 番号: 134332-63-1
分子式: C₂₃H₂₅NO₄
分子量:379.45

保管条件:

Please store the product under the recommended conditions in the Certificate of Analysis.

Phenoxan 構造式

CAS 番号: 134332-63-1

価格（税別）

Amount: USD 0.00

* アイテムを追加する前、数量をご選択ください

Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

生物活性
- 製品説明
- IC₅₀ & Target
- Cellular Effect
- 体外実験
化学情報
プロトコル (2)
純度とドキュメンテーション
参考文献
Calculators
Help & FAQs
関連分類
Bioz

生物活性

製品説明

IC₅₀ & Target

HIV-1

6.6 nM (ID₅₀)

Cytochrome P450 アイソフォーム固有の製品をすべて表示 :

HIV アイソフォーム固有の製品をすべて表示 :

Cellular Effect

Cell Line	Type	Value	Description	References
MT4	EC50	6.6 nM	Inhibition of HIV-1-induced cytopathogenicity in MT-4 cells assessed via ³H-thymidine incorporation into cellular DNA after 4 days post-infection with a 20 h thymidine incubation period. Inhibition of HIV-1-induced cytopathogenicity in MT-4 cells assessed via ³H-thymidine incorporation into cellular DNA after 4 days post-infection with a 20 h thymidine incubation period.	1429243
MT4	CC₅₀	> 6600 nM	Cytotoxicity against MT-4 cells assessed via ³H-thymidine incorporation into cellular DNA after 4 days post-infection with a 20 h thymidine incubation period. Cytotoxicity against MT-4 cells assessed via ³H-thymidine incorporation into cellular DNA after 4 days post-infection with a 20 h thymidine incubation period.	1429243
H9	CC₅₀	6600 nM	Cytotoxicity against human lymphoblastoid H9 cells assessed via ³H-thymidine incorporation measurement. Cytotoxicity against human lymphoblastoid H9 cells assessed via ³H-thymidine incorporation measurement.	1429243
MOLT-3	CC₅₀	6600 nM	Cytotoxicity against human lymphoblastoid Molt-3 cells assessed via ³H-thymidine incorporation measurement. Cytotoxicity against human lymphoblastoid Molt-3 cells assessed via ³H-thymidine incorporation measurement.	1429243
Jurkat	CC₅₀	66000 nM	Cytotoxicity against human lymphoblastoid Jurkat cells assessed via ³H-thymidine incorporation measurement. Cytotoxicity against human lymphoblastoid Jurkat cells assessed via ³H-thymidine incorporation measurement.	1429243
MOLT-4	CC₅₀	66000 nM	Cytotoxicity against human lymphoblastoid Molt-4 clone 8 cells assessed via ³H-thymidine incorporation measurement. Cytotoxicity against human lymphoblastoid Molt-4 clone 8 cells assessed via ³H-thymidine incorporation measurement.	1429243
Raji	CC₅₀	66000 nM	Cytotoxicity against human lymphoblastoid Raji cells assessed via ³H-thymidine incorporation measurement. Cytotoxicity against human lymphoblastoid Raji cells assessed via ³H-thymidine incorporation measurement.	1429243

体外実験

Phenoxan completely inhibits HIV-1-induced cytopathogenicity in MT-4 cells at a concentration of 6.6 μM, exhibits no cytotoxicity at concentrations > 6600 nM, and has a selectivity index > 10³^[2].
Phenoxan does not inhibit HIV-1-induced syncytium formation at a concentration of 6.6 μM in a co-culture system of Molt-4 clone 8 cells and HIV-1_IIIB-infected Molt-4 cells^[2].
Phenoxan inhibits the activity of purified HIV-1_IIIB reverse transcriptase with an IC₅₀ of 376 μM, a concentration that is much higher than that required for its anti-HIV-1 activity in MT-4 cells^[2].
Phenoxan (6600-66000 nM) exhibits toxicity to H9 and Molt-3 cells at a concentration of 6600 nM, and to Jurkat, Molt-4 clone 8 and Raji cells at a concentration of 66000 nM^[2].
Phenoxan (compound 1) (40 μg) inhibits the growth of specific fungal strains, with a minimum inhibitory concentration of 19 ng/mL against Ustilago maydis, and shows no activity against the tested bacteria and yeasts^[1].
Phenoxan (2.2 ng/mL; 4 minutes) potently inhibits NADH oxidation in bovine heart submitochondrial particles, with an IC₅₀ of 5.8 nM (2.2 ng/mL)^[1].
Phenoxan (25 μg/mL) completely inhibits NADH-induced reduction of cytochromes aa₃, b, and c + c₁ in bovine heart submitochondrial particles^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.

化学情報

CAS 番号 134332-63-1
分子量 379.45
分子式 C₂₃H₂₅NO₄
SMILES

CCC1=C(C2=COC(CC/C(C)=C/C3=CC=CC=C3)=N2)OC(OC)=C(C1=O)C
Structure Classification
- Alkaloids
- Other Alkaloids
Initial Source
- Microorganisms
myxobacteria
輸送条件

Room temperature in continental US; may vary elsewhere.
保管条件

Please store the product under the recommended conditions in the Certificate of Analysis.

プロトコル

All Signal Pathway Related Cell Biology

Research Protocol for Infectious Diseases
Infectious-disease experiments test how pathogens interact with host barriers, innate immune receptors, inflammatory signaling, pathogen replication, and tissue injury; pattern-recognition receptors such as TLRs, RIG-I-like receptors, NOD-like receptors, and inflammasomes detect microbial molecules and activate NF-κB, interferon, and cytokine responses. The central hypothesis is that infection severity reflects the balance between pathogen burden and host response: protective inflammation restricts pathogen growth, whereas excessive or mislocalized inflammation contributes to tissue damage and disease phenotype. Unresolved questions include which host pathways are protective versus pathogenic, why some infection models fail to translate to human disease, and which combined readouts best predict clinically relevant infection outcomes.
Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.

純度とドキュメンテーション

取扱説明書 (2659 KB)

参考文献

[1]. Kunze B, et al. Phenoxan, a new oxazole-pyrone from myxobacteria: production, antimicrobial activity and its inhibition of the electron transport in complex I of the respiratory chain. J Antibiot (Tokyo). 1992 Sep;45(9):1549-52.

[2]. Jurkiewicz E, et al. Three New Potent HIV-1 Inhibitors from Myxobacteria. Antiviral Chemistry and Chemotherapy. 1992;3(4):189-193.

[3]. Serebrianyĭ AM, et al. [Stimulated repopulation as a basis of antimutagenesis and the adaptive response in plants]. Genetika. 2002 Mar;38(3):340-6.

Calculators

モル濃度計算機

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

濃度濃度

体積体積

分子量分子量

希釈計算機

濃度 _(開始) × 体積 _(開始) = 濃度 _(終了) × 体積 _(終了)

濃度 _(開始) 濃度 _(開始)

体積 _(開始) 体積 _(開始)

濃度 _(終了) 濃度 _(終了)

体積 _(終了) 体積 _(終了)

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Bioz

Keywords

MOQ

Minimum order quantity

100 mg

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生物活性
- 製品説明
- IC₅₀ & Target
- 体外実験
化学情報
プロトコル
純度とドキュメンテーション
参考文献

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Phenoxan

Cytochrome P450 アイソフォーム固有の製品をすべて表示

HIV アイソフォーム固有の製品をすべて表示