Cat. No.: HY-150190

F5446

1 Cited Publications

고객리뷰

Based on 1 publication(s) in Google Scholar

F5446 (Compound 1) is a selective small molecule inhibitor of SUV39H1 methyltransferase. F5446 decreases H3K9me3 deposition at the FAS promoter, increases Fas expression and increases colorectal carcinoma cell sensitivity to FasL-induced apoptosis in vitro. F5446 suppresses human colon tumor xenograft growth in vivo.

연구목적의 판매만을 진행합니다. 환자를 대상으로 한 판매는 하지 않습니다.

Purity: 99.73%
CAS No.: 2304465-89-0
화학식: C₂₆H₁₇ClN₂O₈S
분자량:552.94

보관:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

F5446 Chemical Structure

CAS No.: 2304465-89-0

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- 제품 설명
- In Vitro
- In Vivo
Chemical Information
Publications (1)
용액&용해도
- In Vitro
- In Vivo
순도&문서
References
Help & FAQs
Classifications
Bioz

Biological Activity

제품 설명

All Histone Methyltransferase Isoforms :

In Vitro

F5446 (0-1 μM, 2 days) has an EC₅₀ of 4.96x10^-7 M against recombinant human SUV39H1 and induces apoptotic cell death in the SW620 and LS411N cells^[1].
F5446 (0-250 nM, 3 days) up-regulates tumor cell surface Fas expression and increases FasL- induced apoptosis in SW620 and LS411N cells^[1]^[2].
F5446 (100 or 250 nM, 48 h) inhibits SUV39H1 expression and induces human colon tumor cell cycle arrest and apoptosis^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Apoptosis Analysis^[1]

Cell Line:

SW620 and LS411N cells
Concentration:

0-1 μM
Incubation Time:

2 days
Result:

Showed the percentage of apoptotic cell death for the SW620 and LS411N cells at various concentrations.

Apoptosis Analysis^[2]

Cell Line:

SW620 and LS411N cells
Concentration:

100 or 250 nM
Incubation Time:

48 h
Result:

Induced cell cycle arrest at the S phase in a concentration-dependent manner in both SW620 and LS411N cells.

In Vivo

IF5446 (10 mg/kg, s.c.,every two days for 14 days) inhibits SUV39H1 and colon tumor growth at least in part through inhibiting H3K9me3 to increase the expression part through inhibiting H3K9me3 to increase the expression level of granzyme B , perforin , Fasl and IFNy in tumor-infiltrating CTLs in the tumor microenvironment in mice bearing MC38 and CT26 tumor^[1]^[2].
IF5446 (10 and 20 mg/kg, s.c.,every two days for 14 days) increases T-cell effector expression to suppress colon carcinoma growth^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Mice bearing MC38 and CT26 tumor^[1]^[2]^[3]
Dosage:

10 mg/kg
Administration:

s.c.,every two days for 14 days
Result:

Suppressed the tumor growth in a time-dependent manner and significantly increased the expression levels of granzymeB, perforin, FasL and IFNγ.

Chemical Information

CAS No. 2304465-89-0
Appearance Solid
분자량 552.94
화학식 C₂₆H₁₇ClN₂O₈S
Color Orange to reddish brown
SMILES

O=C(C1=CC(C2=C(C3=O)NC=C2C(OCC4=CC=CC=C4)=O)=C(N1S(=O)(C5=CC=C(C=C5)Cl)=O)C3=O)OC
선적

Room temperature in continental US; may vary elsewhere.

보관

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Publications (1)

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Journal Impact Factor

Cardiovasc Res

Suppressor of variegation 3-9 homolog 1 deficiency attenuates cardiac fibrosis and rescues heart failure through TACC2. [Abstract]

2026 Mar 20:cvag066. PMID: 41861065

용액&용해도

In Vitro:

DMSO : 100 mg/mL (180.85 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.8085 mL

9.0426 mL

18.0851 mL

5 mM

0.3617 mL

1.8085 mL

3.6170 mL

10 mM

0.1809 mL

0.9043 mL

1.8085 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

몰농도 계산기

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

농도 희석 계산기

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Working Solution

For the following dissolution methods, please prepare the working solution directly:

It is recommended to prepare fresh solutions and use them promptly within a short period of time.

The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 15% Cremophor EL 85% Saline

Solubility: 2 mg/mL (3.62 mM); Suspended solution; Need ultrasonic

Protocol 2

Add each solvent one by one: 10% Cremophor EL in PBS

Solubility: 1.82 mg/mL (3.29 mM); Suspended solution; Need ultrasonic and warming and heat to 60°C

순도&문서

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (286 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Chunwan Lu, et al. Small molecule histone methyltransferase SUV39H1 inhibitor and uses thereof. US10577371B2. 2018.

[2]. Lu C, et al. SUV39H1 regulates human colon carcinoma apoptosis and cell cycle to promote tumor growth. Cancer Lett. 2020 Apr 28;476:87-96. [Content Brief]

[3]. Lu C, et al. SUV39H1 Represses the Expression of Cytotoxic T-Lymphocyte Effector Genes to Promote Colon Tumor Immune Evasion. Cancer Immunol Res. 2019 Mar;7(3):414-427. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	1.8085 mL	9.0426 mL	18.0851 mL	45.2129 mL
	5 mM	0.3617 mL	1.8085 mL	3.6170 mL	9.0426 mL
	10 mM	0.1809 mL	0.9043 mL	1.8085 mL	4.5213 mL
	15 mM	0.1206 mL	0.6028 mL	1.2057 mL	3.0142 mL
	20 mM	0.0904 mL	0.4521 mL	0.9043 mL	2.2606 mL
	25 mM	0.0723 mL	0.3617 mL	0.7234 mL	1.8085 mL
	30 mM	0.0603 mL	0.3014 mL	0.6028 mL	1.5071 mL
	40 mM	0.0452 mL	0.2261 mL	0.4521 mL	1.1303 mL
	50 mM	0.0362 mL	0.1809 mL	0.3617 mL	0.9043 mL
	60 mM	0.0301 mL	0.1507 mL	0.3014 mL	0.7535 mL
	80 mM	0.0226 mL	0.1130 mL	0.2261 mL	0.5652 mL
	100 mM	0.0181 mL	0.0904 mL	0.1809 mL	0.4521 mL

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F5446

Reprogramming Compound Library

Anti-Blood Cancer Compound Library

Anti-Colorectal Cancer Compound Library

Highly Selective Inhibitors Library

Highly Selective Activators Library

Cell Death Library

Anti-Hematopathy Compound Library

Bioactive Compound Library Max

Bioactive Compound Library

Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

Methylation Compound Library

High-Throughput Bioactive Compound Library

Posttranslational Modification Library

Apoptosis Compound Library

Epigenetics Compound Library

Histone Modification Research Compound Library

Anti-Cancer Compound Library

Publications Citing Use of MedChemExpress (MCE) F5446

All Histone Methyltransferase Isoforms

Biological Activity

Chemical Information

Publications (1)

용액&용해도

순도&문서

References

Help & FAQs

Complete Stock Solution Preparation Table

F5446

Reprogramming Compound Library

Anti-Blood Cancer Compound Library

Anti-Colorectal Cancer Compound Library

Highly Selective Inhibitors Library

Highly Selective Activators Library

Cell Death Library

Anti-Hematopathy Compound Library

Bioactive Compound Library Max

Bioactive Compound Library

Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

Methylation Compound Library

High-Throughput Bioactive Compound Library

Posttranslational Modification Library

Apoptosis Compound Library

Epigenetics Compound Library

Histone Modification Research Compound Library

Anti-Cancer Compound Library

Publications Citing Use of MedChemExpress (MCE) F5446

All Histone Methyltransferase Isoforms

Biological Activity

Chemical Information

Publications (1)

용액&용해도

순도&문서

References

Help & FAQs

Complete Stock Solution Preparation Table

F5446 Related Classifications

HY-150190 Related Classifications