NSC668394
Based on 5 publication(s) in Google Scholar
NSC668394 is a potent ezrin (Thr567) phosphorylation inhibitor, with a Kd of 12.59 μM. NSC668394 inhibit ezrin T567 phosphorylation caused by PKCΙ primarily via their binding to ezrin. NSC668394 can be used to prevent tumor metastasis.
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- Purity: 99.31%
- CAS No.: 382605-72-3
- 화학식: C17H12Br2N2O3
- 분자량:452.10
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보관:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Chemical Information
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용액&용해도
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Publications Citing Use of MedChemExpress (MCE) NSC668394
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Biological Activity
NSC668394 (10 μM; pretreated for 15 min) inhibits ezrin T567 phosphorylation (IC50=8.1 μM) and actin binding in vitro[1].
NSC668394 (1-10 μM; 2-6 h) inhibits ezrin-mediated invasion by K7M2 osteosarcoma (OS) cells on the HUVEC monolayer[1].
NSC668394 (20 μM) causes significant decrease in growth in JM1 and JM2 rat hepatoma cell lines[2].
NSC668394 (10 μM) reduces cell motility phenotypes in zebrafish[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:K7M2 OS cells
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Concentration:10 μM
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Incubation Time:6 hours
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Result:Inhibited T567 phosphorylation and actin binding of endogenous ezrin without altering cellular ezrin levels.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Female BALB/c and SCID/Beige mice were injected with K7M2 or MNNG-HOS tumor cells[1]
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Dosage:0.226 mg/kg/day
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Administration:I.p. 5-days a week for 66 days
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Result:Showed an increase in survival.
Decreased the number of the green fluorescent protein (GFP)-expressing metastatic foci in the lung tissues.
Chemical Information
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CAS No. 382605-72-3
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Appearance Solid
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분자량 452.10
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화학식 C17H12Br2N2O3
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Color Orange to red
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SMILES
O=C(C=C1NCCC2=CC(Br)=C(O)C(Br)=C2)C3=C(N=CC=C3)C1=O
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선적
Room temperature in continental US; may vary elsewhere.
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보관
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (5)
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Journal Impact Factor
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Most Recent
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Adv Sci (Weinh)
2026 Mar;13(16):e13310. PMID: 41589654
NSC668394 purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2026 Mar;13(16):e13310. [Abstract]
NSC668394 (5 µM; 0.5-4 h) reduced phosphorylated ERM (pERM) levels in HT-1080 cells.
NSC668394 purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2026 Mar;13(16):e13310. [Abstract]
NSC668394 (5 µM; 12 h) decreased lipid peroxidation levels in HT-1080 cells treated with Erastin.
NSC668394 purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2026 Mar;13(16):e13310. [Abstract]
Cell death measurement of HT-1080 cells treated with Erastin (5 µM) in combination with NSC668394 (2-5 µM; 15-24 h).
NSC668394 purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2026 Mar;13(16):e13310. [Abstract]
Cell death measurement of HT-1080 cells treated with NSC668394 (5 µM; 18 h).
NSC668394 purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2026 Mar;13(16):e13310. [Abstract]
Representative Hoechst and PI staining images of HT-1080 cells treated with NSC668394 (5 μM).
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bioRxiv
Phactr4 influences macrophage lamellipodial structure and dynamics through Arp2/3 complex and Ezrin regulation. [Abstract]2025 May 14:2025.05.13.653717. PMID: 40462895 -
용액&용해도
DMSO : 50 mg/mL (110.60 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (5.53 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
순도&문서
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Data Sheet (275 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
[1]. Bulut G, et, al. Small molecule inhibitors of ezrin inhibit the invasive phenotype of osteosarcoma cells. Oncogene. 2012 Jan 19;31(3):269-81. [Content Brief]
[2]. Xue Y, et, al. Phosphorylated Ezrin (Thr567) Regulates Hippo Pathway and Yes-Associated Protein (Yap) in Liver. Am J Pathol. 2020 Jul;190(7):1427-1437. [Content Brief]
[3]. Çelik H, et, al. Ezrin Inhibition Up-regulates Stress Response Gene Expression. J Biol Chem. 2016 Jun 17;291(25):13257-70. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.2119 mL | 11.0595 mL | 22.1190 mL | 55.2975 mL |
| 5 mM | 0.4424 mL | 2.2119 mL | 4.4238 mL | 11.0595 mL | |
| 10 mM | 0.2212 mL | 1.1059 mL | 2.2119 mL | 5.5297 mL | |
| 15 mM | 0.1475 mL | 0.7373 mL | 1.4746 mL | 3.6865 mL | |
| 20 mM | 0.1106 mL | 0.5530 mL | 1.1059 mL | 2.7649 mL | |
| 25 mM | 0.0885 mL | 0.4424 mL | 0.8848 mL | 2.2119 mL | |
| 30 mM | 0.0737 mL | 0.3686 mL | 0.7373 mL | 1.8432 mL | |
| 40 mM | 0.0553 mL | 0.2765 mL | 0.5530 mL | 1.3824 mL | |
| 50 mM | 0.0442 mL | 0.2212 mL | 0.4424 mL | 1.1059 mL | |
| 60 mM | 0.0369 mL | 0.1843 mL | 0.3686 mL | 0.9216 mL | |
| 80 mM | 0.0276 mL | 0.1382 mL | 0.2765 mL | 0.6912 mL | |
| 100 mM | 0.0221 mL | 0.1106 mL | 0.2212 mL | 0.5530 mL |
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