TCID
Based on 6 publication(s) in Google Scholar
TCID (4,5,6,7-Tetrachloroindan-1,3-dione) is a potent and selective neuronal ubiquitin C-terminal hydrolase (UCH-L3) inhibitor with an IC50 of 0.6 μM. TCID diminishes glycine transporter GlyT2 ubiquitination in brainstem and spinal cord primary neurons.
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- Purity: 99.78%
- CAS No.: 30675-13-9
- 화학식: C9H2Cl4O2
- 분자량:283.92
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보관:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
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Biological Activity
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Chemical Information
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용액&용해도
- 순도&문서
- References
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Help & FAQs
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Cell Cycle/DNA Damage Compound Library
HY-L004
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Anti-Aging Compound Library
HY-L034
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Ubiquitination Compound Library
HY-L050
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Metabolic Enzyme Compound Library
HY-L146
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Protease Inhibitor Library
HY-L147
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Highly Selective Inhibitors Library
HY-L158
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Bioactive Compound Library Max
HY-L181
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Bioactive Compound Library
HY-L001
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High-Throughput Bioactive Compound Library
HY-L205
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Posttranslational Modification Library
HY-L226
Publications Citing Use of MedChemExpress (MCE) TCID
More- Cancer Cell. 2026 Feb 9;44(2):306-320.e7. [Abstract]
- Nat Commun. 2025 Feb 27;16(1):2039. [Abstract]
- J Nanobiotechnology. 2023 Mar 14;21(1):88. [Abstract]
- Redox Biol. 2026 May:92:104086. [Abstract]
- Environ Pollut. 2025 Aug 8:384:126969. [Abstract]
- Anim Cells Syst. 2024 May 11;28(1):237-250. [Abstract]
Customer Validation & Images
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RT-PCR
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WB
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Cell Imaging/Staining
Biological Activity
IC50: 0.6 μM (UCH-L3) and 75 μM (UCH-L1)[1]
TCID (4,5,6,7-Tetrachloroindan-1,3-dione; compound 11) inhibits UCH-L1 with an IC50 of 75 μM[1].
TCID (10 μM; 1, 2 hours) diminishes glycine transporter GlyT2 ubiquitination in brainstem and spinal cord primary neurons[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 30675-13-9
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Appearance Solid
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분자량 283.92
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화학식 C9H2Cl4O2
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Color Light yellow to yellow
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SMILES
O=C1CC(C2=C1C(Cl)=C(Cl)C(Cl)=C2Cl)=O
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Synonyms
4,5,6,7-Tetrachloroindan-1,3-dione
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선적
Room temperature in continental US; may vary elsewhere.
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보관
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Publications (6)
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Journal Impact Factor
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Most Recent
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Cancer Cell
Ubiquitination-directed cytosolic DNA degradation governs cGAS-STING-mediated immune response to DNA damage. [Abstract]2026 Feb 9;44(2):306-320.e7. PMID: 41512867 -
Nat Commun
The adaptor protein AP-3β disassembles heat-induced stress granules via 19S regulatory particle in Arabidopsis. [Abstract]2025 Feb 27;16(1):2039. PMID: 40016204 -
J Nanobiotechnology
Mechanical force induces macrophage-derived exosomal UCHL3 promoting bone marrow mesenchymal stem cell osteogenesis by targeting SMAD1. [Abstract]2023 Mar 14;21(1):88. PMID: 36915132
TCID purchased from MedChemExpress. Usage Cited in: J Nanobiotechnology. 2023 Mar 14;21(1):88. [Abstract]
qRT‒PCR of the mRNA expression of osteogenesis genes in BMSCs after treatment with UCHL3 inhibitor TCID (10 μM), and DMSO as a control.
TCID purchased from MedChemExpress. Usage Cited in: J Nanobiotechnology. 2023 Mar 14;21(1):88. [Abstract]
Western blotting of RUNX2 and UCHL3 protein levels in BMSCs after treatment with UCHL3 inhibitor TCID (10 μM), and DMSO as a control.
TCID purchased from MedChemExpress. Usage Cited in: J Nanobiotechnology. 2023 Mar 14;21(1):88. [Abstract]
ALP staining and Alizarin red staining in BMSCs after treatment with TCID (10 μM), and DMSO as a control.
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Redox Biol
USP20 governs tyrosine kinase inhibitors resistance through ferroptosis evasion by targeting GPX4 in cancers. [Abstract]2026 May:92:104086. PMID: 41844497 -
Environ Pollut
UCHL3-mediated proteasomal degradation of GOPC drives microcystin-LR-induced acrosomal dysgenesis. [Abstract]2025 Aug 8:384:126969. PMID: 40784473 -
Anim Cells Syst
Blocking SLC7A11 attenuates the proliferation of esophageal squamous cell carcinoma cells. [Abstract]2024 May 11;28(1):237-250. PMID: 38741950
용액&용해도
DMSO : 20 mg/mL (70.44 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (7.33 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
순도&문서
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Data Sheet (283 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Liu Y, et al. Discovery of inhibitors that elucidate the role of UCH-L1 activity in the H1299 lung cancer cellline. Chem Biol. 2003 Sep;10(9):837-46. [Content Brief]
[2]. de Juan-Sanz J, et al. Constitutive endocytosis and turnover of the neuronal glycine transporter GlyT2 is dependent on ubiquitination of a C-terminal lysine cluster. PLoS One. 2013;8(3):e58863. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.5221 mL | 17.6106 mL | 35.2212 mL | 88.0530 mL |
| 5 mM | 0.7044 mL | 3.5221 mL | 7.0442 mL | 17.6106 mL | |
| 10 mM | 0.3522 mL | 1.7611 mL | 3.5221 mL | 8.8053 mL | |
| 15 mM | 0.2348 mL | 1.1740 mL | 2.3481 mL | 5.8702 mL | |
| 20 mM | 0.1761 mL | 0.8805 mL | 1.7611 mL | 4.4026 mL | |
| 25 mM | 0.1409 mL | 0.7044 mL | 1.4088 mL | 3.5221 mL | |
| 30 mM | 0.1174 mL | 0.5870 mL | 1.1740 mL | 2.9351 mL | |
| 40 mM | 0.0881 mL | 0.4403 mL | 0.8805 mL | 2.2013 mL | |
| 50 mM | 0.0704 mL | 0.3522 mL | 0.7044 mL | 1.7611 mL | |
| 60 mM | 0.0587 mL | 0.2935 mL | 0.5870 mL | 1.4675 mL |
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