N-Nitrosodibutylamine
Based on 1 publication(s) in Google Scholar
N-Nitrosodibutylamine (N-Nitroso-di-n-butylamine) is a nitrosamine enriched in the drinking water. N-Nitrosodibutylamine regulates caspase pathway. N-Nitrosodibutylamine induces Apoptosis. N-Nitrosodibutylamine induces hepatocarcinoma and oxidative DNA damage.
For research use only. We do not sell to patients.
- Purity: 99.93%
- CAS No.: 924-16-3
- Formula: C8H18N2O
- Molecular Weight:158.24
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Storage:Pure form -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Apoptosis Compound Library
HY-L003
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Anti-Cancer Compound Library
HY-L025
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Tumorigenesis-Related Compound Library
HY-L100
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Human Metabolite Library
HY-L123
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Cell Death Library
HY-L162
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Bioactive Compound Library Max
HY-L181
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MCE Bioactive Compound Library
HY-L001V
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Bioactive Compound Library
HY-L001
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Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library
HY-L199
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High-Throughput Bioactive Compound Library
HY-L205
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Mass Spectrometry Human Metabolite Library
HY-L215
Publications Citing Use of MedChemExpress (MCE) N-Nitrosodibutylamine
MoreAll Caspase Isoforms
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Biological Activity
N-Nitrosodibutylamine (1-3.5 mM, 24-72 h) induces apoptosis in HepG2 cells via the caspase dependent pathway[2]. N-Nitrosodibutylamine (3 mM, 24 h) induces oxidative DNA damage in HepG2 cells[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 924-16-3
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Appearance Liquid (Density: 0.9009 g/cm3)
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Molecular Weight 158.24
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Formula C8H18N2O
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Color Light yellow to yellow
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SMILES
CCCCN(CCCC)N=O
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Synonyms
N-Nitroso-di-n-butylamine
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Pure form -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (1)
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Journal Impact Factor
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Most Recent
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Water Res
Trihalomethanes formation enhanced by manganese chlorination and deposition in plastic drinking water pipes. [Abstract]2021 Oct 1:204:117582. PMID: 34474250
Solvent & Solubility
DMSO : 100 mg/mL (631.95 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 6.25 mg/mL (39.50 mM); Clear solution
This protocol yields a clear solution of ≥ 6.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (62.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 6.25 mg/mL (39.50 mM); Clear solution
This protocol yields a clear solution of ≥ 6.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (62.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (270 KB)
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SDS (617 KB)
- English - EN (617 KB)
- Français - FR (617 KB)
- Deutsch - DE (617 KB)
- Norwegian - NO (617 KB)
- Español - ES (617 KB)
- Swedish - SV (617 KB)
- Italian - IT (617 KB)
- Korean - KR (617 KB)
- Portuguese - PT (617 KB)
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Handling Instructions (2659 KB)
References
[1]. Chao Zhao, et al. Distribution of N-nitrosamines in Drinking Water and Human Urinary Excretions in High Incidence Area of Esophageal Cancer in Huai'an, China. Chemosphere. 2019 Nov;235:288-296. [Content Brief]
[2]. García A, et al. N-Nitrosopiperidine and N-Nitrosodibutylamine induce apoptosis in HepG2 cells via the caspase dependent pathway. Cell Biol Int. 2009 Dec;33(12):1280-6. [Content Brief]
[3]. García A, et al. Organosulfur compounds alone or in combination with vitamin C protect towards N-nitrosopiperidine- and N-nitrosodibutylamine-induced oxidative DNA damage in HepG2 cells. Chem Biol Interact. 2008 May 9;173(1):9-18. [Content Brief]
[4]. Brittebo EB, et al. Tissue-specificity of N-nitrosodibutylamine metabolism in Sprague-Dawley rats. Chem Biol Interact. 1982 Jan;38(2):231-42. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 6.3195 mL | 31.5976 mL | 63.1951 mL | 157.9879 mL |
| 5 mM | 1.2639 mL | 6.3195 mL | 12.6390 mL | 31.5976 mL | |
| 10 mM | 0.6320 mL | 3.1598 mL | 6.3195 mL | 15.7988 mL | |
| 15 mM | 0.4213 mL | 2.1065 mL | 4.2130 mL | 10.5325 mL | |
| 20 mM | 0.3160 mL | 1.5799 mL | 3.1598 mL | 7.8994 mL | |
| 25 mM | 0.2528 mL | 1.2639 mL | 2.5278 mL | 6.3195 mL | |
| 30 mM | 0.2107 mL | 1.0533 mL | 2.1065 mL | 5.2663 mL | |
| 40 mM | 0.1580 mL | 0.7899 mL | 1.5799 mL | 3.9497 mL | |
| 50 mM | 0.1264 mL | 0.6320 mL | 1.2639 mL | 3.1598 mL | |
| 60 mM | 0.1053 mL | 0.5266 mL | 1.0533 mL | 2.6331 mL | |
| 80 mM | 0.0790 mL | 0.3950 mL | 0.7899 mL | 1.9748 mL | |
| 100 mM | 0.0632 mL | 0.3160 mL | 0.6320 mL | 1.5799 mL |
N-Nitrosodibutylamine Related Classifications
HY-131113 Related Classifications
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