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Cat. No.: HY-170674

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ND-L11B free base

ND-L11B free base is an NSD2 degrader with a K_d value of 8.77 μM, degrading the NSD2-long and RE-IIBP isoforms in multiple myeloma cells with DC₅₀ values of 1.48 μM and 0.8 μM, respectively. ND-L11B free base reduces H3K36me2 levels and inhibits the proliferation of multiple myeloma cells. ND-L11B free base serves as a starting point for the further development of NSD2-targeted compounds and is also a chemical knockdown tool for elucidating the multiple functions of NSD2 isoforms. ND-L11B free base can be used for research on multiple myeloma.

For research use only. We do not sell to patients.

Formula: C₃₇H₅₁F₃N₁₀O₂
Molecular Weight:724.86

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (278 KB)

ND-L11B free base Chemical Structure

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Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
Chemical Information
Purity & Documentation
References
Calculators
Help & FAQs
Classifications
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Biological Activity

Description

IC₅₀ & Target

^[1]

NSD2

8.77 μM (Kd)

All Histone Methyltransferase Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
KMS-11	DC₅₀	1.48 μM	Degradation of NSD2-long isoform in human KMS11 multiple myeloma cells assessed via immunoblotting. Degradation of NSD2-long isoform in human KMS11 multiple myeloma cells assessed via immunoblotting.	39705735
KMS-11	DC₅₀	0.80 μM	Degradation of RE-IIBP isoform in human KMS11 multiple myeloma cells assessed via immunoblotting. Degradation of RE-IIBP isoform in human KMS11 multiple myeloma cells assessed via immunoblotting.	39705735
KMS-11	IC50	0.90 μM	Inhibition of cell proliferation in human KMS11 multiple myeloma cells assessed as reduction in cell viability after 12 days by cell viability assay. Inhibition of cell proliferation in human KMS11 multiple myeloma cells assessed as reduction in cell viability after 12 days by cell viability assay.	39705735
HEK-293T	IC50	> 100 μM	Inhibition of cell viability in human 293T embryonic kidney cells assessed after 8 days by cell viability assay. Inhibition of cell viability in human 293T embryonic kidney cells assessed after 8 days by cell viability assay.	39705735

In Vitro

Anti-cancer

ND-L11B free base (0.09-5.0 μM; 6-48 h) simultaneously degrades both NSD2-long and RE-IIBP isoforms in KMS11 multiple myeloma cells, with DC₅₀ values of 1.48 μM and 0.80 μM, respectively, and induces time-dependent NSD2 degradation from 6 to 48 h^[1].
ND-L11B free base (0.63-2.50 μM for KMS11 cells; 2.0-8.0 μM for 22RV1 cells) downregulates H₃K₃₆ methylation levels in KMS11 multiple myeloma cells and 22RV1 prostate cancer cells^[1].
ND-L11B free base (12-day exposure of KMS11 cells; 8-day exposure of 293T cells) potently inhibits the proliferation of KMS11 multiple myeloma cells with an IC₅₀ of 0.90 μM, while showing very low cytotoxicity against non-malignant human embryonic kidney 293T cells (IC₅₀ > 100 μM)^[1].
ND-L11B free base (1.25-2.50 μM; 2 days) downregulates the expression of NSD2-regulated cancer-related genes in KMS11 multiple myeloma cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[1]

Cell Line:

KMS11 multiple myeloma cells
Concentration:

0.09, 0.13, 0.20, 0.29, 0.44, 0.66, 0.99, 1.48, 2.22, 3.33, 5.0 μM; 5 μM (time-dependent assay)
Incubation Time:

6, 12, 24, 36, 48 h (5 μM concentration)
Result:

Induced simultaneous concentration-dependent degradation of NSD2-long and RE-IIBP isoforms, with DC₅₀ values of 1.48 μM (NSD2-long) and 0.80 μM (RE-IIBP), and a maximum degradation (D_max) of approximately 80%.
Induced time-dependent degradation of NSD2 protein over 6 to 48 hours.

Real Time qPCR^[1]

Cell Line:

KMS11 multiple myeloma cells
Concentration:

1.25, 2.50 μM
Incubation Time:

2 days
Result:

Downregulated the expression of NSD2-regulated oncogenes and cancer-related genes including JAM2, MET, PAK1, RRAS2, BACE2, CA2, IGF2BP2, and CR2.

Related Protocols :

Chemical Information

Molecular Weight 724.86
Formula C₃₇H₅₁F₃N₁₀O₂
SMILES

O=C(NCCCCCCCCCCCN)COC1=CC(C2=NC=C(N3C[C@](CC(F)F)(N)CCC3)C(CN4C=NC5=C(N)N=CN=C45)=C2)=CC=C1F
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (278 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Hu L, et al. Discovery of SET domain-binding primary alkylamine-tethered degraders for the simultaneous degradation of NSD2-long and RE-IIBP isoforms. European journal of medicinal chemistry. 2025 Feb 05;283:117179. [Content Brief]

Calculators

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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100 mg

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