2. Epigenetics
  3. Epigenetic Reader Domain
  4. NI-42

NI-42 

Cat. No.: HY-101121 Purity: 99.79%
COA Handling Instructions

NI-42 (compound 13-d), a structurally orthogonal chemical probe for the BRPFs, is a biased, potent inhibitor of the BRD of the BRPFs (IC50s of BRPF1/2/3=7.9/48/260 nM; Kds of BRPF1/2/3=40/210/940 nM) with excellent selectivity over nonclass IV BRD proteins.

For research use only. We do not sell to patients.

NI-42 Chemical Structure

NI-42 Chemical Structure

CAS No. : 1884640-99-6

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Solid + Solvent
10 mM * 1 mL in DMSO
ready for reconstitution
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Solution
10 mM * 1 mL in DMSO USD 88 In-stock
Solid
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10 mg USD 130 In-stock
25 mg USD 260 In-stock
50 mg USD 400 In-stock
100 mg USD 630 In-stock
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Based on 1 publication(s) in Google Scholar

NI-42 Related Antibodies

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Description

NI-42 (compound 13-d), a structurally orthogonal chemical probe for the BRPFs, is a biased, potent inhibitor of the BRD of the BRPFs (IC50s of BRPF1/2/3=7.9/48/260 nM; Kds of BRPF1/2/3=40/210/940 nM) with excellent selectivity over nonclass IV BRD proteins[1].

IC50 & Target

BRPF1

7.9 nM (IC50)

BRPF2

48 nM (IC50)

BRPF3

260 nM (IC50)

BRPF1

40 nM (Kd)

BRPF2

210 nM (Kd)

BRPF3

940 nM (Kd)

BRD4 (BD1)

4500 nM (IC50)

BRD7

82 nM (IC50)

BRD9

310 nM (IC50)

BRD9

1130 nM (Kd)

In Vitro

NI-42 shows IC50s of 82, 310, and 4500 nM for BRD7, BRD9, and BRD4 (BD1), respectively, and has a Kd of 1130 nM for BRD9[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

NI-42 Related Antibodies
Molecular Weight

353.40

Appearance

Solid

Formula

C18H15N3O3S
CAS No.
SMILES

O=S(C1=CC=C(C#N)C=C1)(NC2=CC=C(N(C)C(C(C)=C3)=O)C3=C2)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (282.97 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.8297 mL 14.1483 mL 28.2965 mL
5 mM 0.5659 mL 2.8297 mL 5.6593 mL
10 mM 0.2830 mL 1.4148 mL 2.8297 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (7.07 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: 2.5 mg/mL (7.07 mM); Suspended solution; Need ultrasonic

*All of the co-solvents are available by MedChemExpress (MCE).
Purity & Documentation

Purity: 99.79%

COA (248 KB) HNMR (259 KB) LCMS (269 KB)

Handling Instructions (2659 KB)
References
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NI-42 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

Keywords:

NI-421884640-99-6NI42NI 42Epigenetic Reader DomainInhibitorinhibitorinhibit

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