Cat. No.: HY-W011303

Phytosphingosine

(Synonyms: 4-Hydroxysphinganine)

4 Cited Publications

Customer Review

Based on 4 publication(s) in Google Scholar

Phytosphingosine is a phospholipid with anti-inflammatory, antibacterial, and anti-cancer activities, which can induce apoptosis. Phytosphingosine is an immune regulator and can be used in the study of inflammatory skin diseases. Phytosphingosine is also an activator of GPR120 with an IC₅₀ value of 33.4 μM and can be used in the study of type II diabetes.

For research use only. We do not sell to patients.

Purity: 99.85%
CAS No.: 554-62-1
Formula: C₁₈H₃₉NO₃
Molecular Weight:317.51

Storage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

Phytosphingosine Chemical Structure

CAS No.: 554-62-1

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (4)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target

Human Endogenous Metabolite

All Endogenous Metabolite Isoforms :

All Free Fatty Acid Receptor Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
Jurkat	IC₅₀	3.75 μM Compound: Phytosphingosine	Inhibition of wild type human Jurkat cells after 18 hrs by MTT assay Inhibition of wild type human Jurkat cells after 18 hrs by MTT assay	[PMID: 17400555]
Jurkat	IC₅₀	4.12 μM Compound: Phytosphingosine	Inhibition of human 2-amino-4-(4-heptyloxyphenyl)-2-methylbutanol-resistant human Jurkat SBR3 mutant cells proliferation after 18 hrs by MTT assay Inhibition of human 2-amino-4-(4-heptyloxyphenyl)-2-methylbutanol-resistant human Jurkat SBR3 mutant cells proliferation after 18 hrs by MTT assay	[PMID: 17400555]
Jurkat	IC₅₀	5.29 μM Compound: Phytosphingosine	Inhibition of 2-amino-4-(4-heptyloxyphenyl)-2-methylbutanol-resistant human Jurkat SBR2 mutant cells proliferation after 18 hrs by MTT assay Inhibition of 2-amino-4-(4-heptyloxyphenyl)-2-methylbutanol-resistant human Jurkat SBR2 mutant cells proliferation after 18 hrs by MTT assay	[PMID: 17400555]
Jurkat	IC₅₀	6.57 μM Compound: Phytosphingosine	Inhibition of 2-amino-4-(4-heptyloxyphenyl)-2-methylbutanol-resistant human Jurkat SBR1 mutant cells proliferation after 18 hrs by MTT assay Inhibition of 2-amino-4-(4-heptyloxyphenyl)-2-methylbutanol-resistant human Jurkat SBR1 mutant cells proliferation after 18 hrs by MTT assay	[PMID: 17400555]

In Vitro

Phytosphingosine (200-400 μg/mL; 60-240 min) can inhibit the growth of Gram-positive and Gram-negative bacteria, yeast, and molds, and significantly inhibit the release of IL-1a, exhibiting anti-inflammatory activity^[1].
Phytosphingosine (1-5 μg/mL; 24-48 h) inhibits lung adenoma cell proliferation by inducing G2/M phase arrest, apoptosis and mitochondria-dependent pathway cell death in A549 and LLC cells, with IC₅₀ values of 4.3 μg/mL and 4.5μg/mL, respectively^[2].
Phytosphingosine (80 μM; 1-10 days) has antibacterial activity and can inhibit the growth ofS. sclerotiorum, S. indica and V. longisporum^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[2]

Cell Line:

A549, LLC, BEAS-2B
Concentration:

1 μg/mL, 2 μg/mL, 3 μg/mL, 4 μg/mL, 5 μg/mL
Incubation Time:

24 h, 48 h
Result:

Significantly inhibited cell proliferation in a concentration- and time-dependent manner.

Cell Cycle Analysis^[2]

Cell Line:

A549
Concentration:

1 μg/mL, 3 μg/mL, 5 μg/mL
Incubation Time:

24 h
Result:

Significantly increased the proportion of G2/M phase cells.

Western Blot Analysis^[2]

Cell Line:

A549
Concentration:

1 μg/mL, 3 μg/mL, 5 μg/mL
Incubation Time:

24 h
Result:

Up-regulated the pro-apoptotic factor Bax protein level and down-regulated the anti-apoptotic factor Bcl-2 protein level.
Promoted the release of cytochrome c to up-regulate caspase 9 and caspase 3 levels, leading to PARP cleavage.

In Vivo

Phytosphingosine (0.5-2 mg/kg; Intraperitoneal injection; once every other day for 10 times) has anti-tumor activity in C57BL/6 mice with lung cancer^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 554-62-1
Appearance Solid
Molecular Weight 317.51
Formula C₁₈H₃₉NO₃
Color White to off-white
SMILES

CCCCCCCCCCCCCC[C@@H](O)[C@@H](O)[C@@H](N)CO
Synonyms

4-Hydroxysphinganine
Structure Classification
- Others
Initial Source
- Endogenous metabolite
- Human Gut Microbiota Metabolites
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Publications (4)

All ELISAHistological Imaging/StainingCell Imaging/StainingWBIF

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Journal Impact Factor

MedComm (2020)

Phytosphingosine Alleviates Cigarette Smoke-Induced Bronchial Epithelial Cell Senescence in Chronic Obstructive Pulmonary Disease by Targeting the Free Fatty Acid Receptor 4. [Abstract]

2025 Aug 29;6(9):e70345. PMID: 40895192

Phytosphingosine purchased from MedChemExpress. Usage Cited in: MedComm (2020). 2025 Aug 29;6(9):e70345. [Abstract]

Phytosphingosine (PHS) (25 mg/kg; p.o.; once daily for 3 weeks) significantly decreased the levels of IL-6, KC, and IL-1β in CS-exposed mice.

Phytosphingosine purchased from MedChemExpress. Usage Cited in: MedComm (2020). 2025 Aug 29;6(9):e70345. [Abstract]

Phytosphingosine (PHS) (25 mg/kg; p.o.; once daily for 3 weeks) reduced infiltration of inflammatory cells around the airways in CS-exposed mice and inflammation scores.

Phytosphingosine purchased from MedChemExpress. Usage Cited in: MedComm (2020). 2025 Aug 29;6(9):e70345. [Abstract]

Phytosphingosine (PHS) (25 mg/kg; p.o.; once daily for 3 weeks) significantly decreased total and various inflammatory cell counts, particularly macrophages, in bronchoalveolar lavage fluid (BALF) of CS-exposed mice.

Phytosphingosine purchased from MedChemExpress. Usage Cited in: MedComm (2020). 2025 Aug 29;6(9):e70345. [Abstract]

Phytosphingosine (PHS) (25 mg/kg; p.o.; once daily for 3 weeks) significantly downregulated p53, p21, and p-Rb in CS-exposed mice.

Phytosphingosine purchased from MedChemExpress. Usage Cited in: MedComm (2020). 2025 Aug 29;6(9):e70345. [Abstract]

Co-immunofluorescence targeting p53 and p21 revealed that PHS treatment reduced the expression of senescent proteins in the airway epithelium of CS-exposed mice.
Chem Biol Interact

Phytosphingosine suppresses gastric cancer through SFRP4/β-catenin axis-mediated Wnt signaling pathway inhibition. [Abstract]

2025 Sep 20:111749. PMID: 40983245
Molecules

Untargeted Metabolomics Using UHPLC-HRMS Reveals Metabolic Changes of Fresh-Cut Potato during Browning Process. [Abstract]

2023 Apr 11;28(8):3375. PMID: 37110608
SSRN

2025 Jun 5.

Solvent & Solubility

In Vitro:

DMSO : 5 mg/mL (15.75 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

3.1495 mL

15.7475 mL

31.4951 mL

5 mM

0.6299 mL

3.1495 mL

6.2990 mL

10 mM

0.3150 mL

1.5748 mL

3.1495 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (7.87 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (7.87 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (7.87 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

Working Solution

For the following dissolution methods, please prepare the working solution directly:

It is recommended to prepare fresh solutions and use them promptly within a short period of time.

The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 50% PEG300 50% Saline

Solubility: 3.33 mg/mL (10.49 mM); Suspended solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (274 KB)
SDS (620 KB)
- English - EN (620 KB)
- Français - FR (620 KB)
- Deutsch - DE (620 KB)
- Norwegian - NO (620 KB)
- Español - ES (620 KB)
- Swedish - SV (620 KB)
- Italian - IT (620 KB)
- Korean - KR (620 KB)
- Portuguese - PT (620 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. 1. Pavicic T, Wollenweber U, Farwick M, et al. Anti‐microbial and‐inflammatory activity and efficacy of phytosphingosine: an in vitro and in vivo study addressing acne vulgaris[J]. International journal of cosmetic science, 2007, 29(3): 181-190. [Content Brief]

[2]. Cai Q, et al. Phytosphingosine inhibits the growth of lung adenocarcinoma cells by inducing G2/M-phase arrest, apoptosis, and mitochondria-dependent pathway cell death in vitro and in vivo. Chem Biol Interact. 2024 Jan 5;387:110795. [Content Brief]

[3]. Glenz R, et al. The major plant sphingolipid long chain base phytosphingosine inhibits growth of bacterial and fungal plant pathogens. Sci Rep. 2022 Jan 20;12(1):1081. [Content Brief]

[4]. Nagasawa T, et al. Phytosphingosine is a novel activator of GPR120. J Biochem. 2018 Jul 1;164(1):27-32. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	3.1495 mL	15.7475 mL	31.4951 mL	78.7377 mL
	5 mM	0.6299 mL	3.1495 mL	6.2990 mL	15.7475 mL
	10 mM	0.3150 mL	1.5748 mL	3.1495 mL	7.8738 mL
	15 mM	0.2100 mL	1.0498 mL	2.0997 mL	5.2492 mL

Phytosphingosine

4 Cited Publications

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Phytosphingosine

Anti-Infection Compound Library

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Publications Citing Use of MedChemExpress (MCE) Phytosphingosine

All Endogenous Metabolite Isoforms

All Free Fatty Acid Receptor Isoforms

Biological Activity

Chemical Information

Publications (4)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Phytosphingosine Related Classifications

HY-W011303 Related Classifications