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  4. Nootkatone

Nootkatone, a neuroprotective agent from Vitis vinifera, has antioxidant and anti-inflammatory effects. Nootkatone improves cognitive impairment in lipopolysaccharide-induced mouse model of Alzheimer's disease.

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CAS No. : 4674-50-4

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10 mM * 1 mL in DMSO
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Customer Review

Based on 4 publication(s) in Google Scholar

Other Forms of Nootkatone:

Nootkatone Related Antibodies

Top Publications Citing Use of Products
Cell Proliferation/Viability Assay
IF
Cell Migration/Invasion Assay
Flow Cytometry
WB

    Nootkatone purchased from MedChemExpress. Usage Cited in: Mol Med. 2025 Jan 16;31(1):13.  [Abstract]

    The cell viability of C6 cells and U251 cells treated with gradient concentrations (0 µM, 100 µM, 200 µM, 300 µM) of Nootkatone detected by CCK8 assays.

    Nootkatone purchased from MedChemExpress. Usage Cited in: Mol Med. 2025 Jan 16;31(1):13.  [Abstract]

    Representative images of PH3 staining (red) in C6 cells after being treated with DMSO or 200 µM Nootkatone for 48 h.

    Nootkatone purchased from MedChemExpress. Usage Cited in: Mol Med. 2025 Jan 16;31(1):13.  [Abstract]

    Representative images of C6 cells and U251 cells treated with 200 µM Nootkatone (NKT) in wound healing assays.

    Nootkatone purchased from MedChemExpress. Usage Cited in: Mol Med. 2025 Jan 16;31(1):13.  [Abstract]

    Quantitative analysis in C6 cells and U251 cells after Nootkatone (NKT) (200 μM) treatment for 48 h.

    Nootkatone purchased from MedChemExpress. Usage Cited in: Mol Med. 2025 Jan 16;31(1):13.  [Abstract]

    The expression of ATF4, CHOP, CHAC1 in U251 cells was detected after being treated with 200 µM Nootkatone (NKT) for 48 h by western blot.

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    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Nootkatone, a neuroprotective agent from Vitis vinifera, has antioxidant and anti-inflammatory effects[1]. Nootkatone improves cognitive impairment in lipopolysaccharide-induced mouse model of Alzheimer's disease[2].

    Cellular Effect
    Cell Line Type Value Description References
    Macrophage IC50
    34 μM
    Compound: 4
    Inhibition of LPS-induced NO production in ddY mouse macrophages assessed as nitrate accumulation after 20 hrs by Griess reagent method
    Inhibition of LPS-induced NO production in ddY mouse macrophages assessed as nitrate accumulation after 20 hrs by Griess reagent method
    		[PMID: 12398545]
    In Vivo

    Nootkatone (10 mg/kg) group shows good performance in behavior experiments including Y-maze test and Morris water maze test. The results of histopathological examination and immunohistochemical analysis showed that LPS induced degeneration of neurons and activation of microglia particularly in hippocampus and Nootkatone (10 mg/kg) reversed these changes. Enzyme linked immunosorbent assay and western blot analysis also demonstrated that the model group had increased expression of IL-1β, IL-6, TNF-α, NLRP3 and NF-κB p65, especially in hippocampus relative to sham-operated group, and Nootkatone (10 mg/kg) decreased the high expression of these inflammatory cytokines[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
    Molecular Weight

    218.33

    Formula

    C15H22O
    CAS No.
    Appearance

    Solid

    Color

    White to yellow

    SMILES

    O=C1C=C2CC[C@@H](C(C)=C)C[C@@]2(C)[C@H](C)C1
    Structure Classification
    Initial Source
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 2 years
    -20°C 1 year
    Solvent & Solubility
    In Vitro: 

    DMSO : 100 mg/mL (458.02 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 4.5802 mL 22.9011 mL 45.8022 mL
    5 mM 0.9160 mL 4.5802 mL 9.1604 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

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    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.5 mg/mL (11.45 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.5 mg/mL (11.45 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 99.81%

    SDS (393 KB)

    COA (247 KB) HNMR (257 KB) RP-HPLC (229 KB) MS (68 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 4.5802 mL 22.9011 mL 45.8022 mL 114.5056 mL
    5 mM 0.9160 mL 4.5802 mL 9.1604 mL 22.9011 mL
    10 mM 0.4580 mL 2.2901 mL 4.5802 mL 11.4506 mL
    15 mM 0.3053 mL 1.5267 mL 3.0535 mL 7.6337 mL
    20 mM 0.2290 mL 1.1451 mL 2.2901 mL 5.7253 mL
    25 mM 0.1832 mL 0.9160 mL 1.8321 mL 4.5802 mL
    30 mM 0.1527 mL 0.7634 mL 1.5267 mL 3.8169 mL
    40 mM 0.1145 mL 0.5725 mL 1.1451 mL 2.8626 mL
    50 mM 0.0916 mL 0.4580 mL 0.9160 mL 2.2901 mL
    60 mM 0.0763 mL 0.3817 mL 0.7634 mL 1.9084 mL
    80 mM 0.0573 mL 0.2863 mL 0.5725 mL 1.4313 mL
    100 mM 0.0458 mL 0.2290 mL 0.4580 mL 1.1451 mL
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    Nootkatone Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Nootkatone4674-50-4(+)-NootkatoneTNF ReceptorInterleukin RelatedEnvironmental PollutantsTumor Necrosis Factor ReceptorTNFRILEnvironmental ContaminantsneuroprotectivecognitiveimpairmentAlzheimer'sdiseaseInhibitorinhibitorinhibit

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