NPD-001
Based on 1 Customer Validation
NPD-001 is a potent Trypanosoma brucei phosphodiesterases 1/2 (TbrPDEB1/2) inhibitor, with IC50 values of 4 and 3 nM, respectively. NPD-001 also inhibits hPDE4. NPD-001 can increase cAMP levels in parasites, prevent cytokinesis, and cause the parasites to form multinucleated, multi-flagellated cells that eventually lyse. NPD-001 can be used for the research of infection.
For research use only. We do not sell to patients.
- Purity: 98.11%
- CAS No.: 469863-16-9
- Formula: C33H40N6O4
- Molecular Weight:584.72
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
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Trypanosoma |
PDE1 4 nM (IC50) |
PDE2 3 nM (IC50) |
hPDE4D |
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| HeLa | IC50 |
>25 μM
Compound: 15; MMV690027
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Cytotoxicity against human HeLa cells assessed as reduction in cell viability after 48 hrs by resazurin dye based fluorescence assay
Cytotoxicity against human HeLa cells assessed as reduction in cell viability after 48 hrs by resazurin dye based fluorescence assay
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[PMID: 30647879] |
| Sf21 | IC50 |
0.004 μM
Compound: 3, PPS54019
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Inhibition of Trypanosoma brucei full length PDEB1 expressed in insect SF21 cells using cAMP as substrate by scintillation proximity assay
Inhibition of Trypanosoma brucei full length PDEB1 expressed in insect SF21 cells using cAMP as substrate by scintillation proximity assay
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[PMID: 23409953] |
NPD-001 inhibits the proliferation of Trypanosoma brucei bloodstream form trypomastigotes, with -log EC50 of 7.7[1].
NPD-001 shows low cytotoxicity against human MRC-5 fibroblasts, with a selectivity index (MRC-5 cells/T. b. brucei) of 316[1].
NPD-001 potently inhibits human PDE4 subtypes (pIC50 = 9.2 for hPDE4B1, 9.3 for hPDE4B2, 9.2 for hPDE4D3)[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 469863-16-9
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Appearance Solid
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Molecular Weight 584.72
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Formula C33H40N6O4
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Color Off-white to light yellow
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SMILES
O=C1N(C2CCCCCC2)N=C(C3=CC=C(OC)C(OCCCCOC4=CC=C(C5=NN=NN5)C=C4)=C3)[C@@]6([H])CC=CC[C@@]16[H]
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 100 mg/mL (171.02 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (270 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.7102 mL | 8.5511 mL | 17.1022 mL | 42.7555 mL |
| 5 mM | 0.3420 mL | 1.7102 mL | 3.4204 mL | 8.5511 mL | |
| 10 mM | 0.1710 mL | 0.8551 mL | 1.7102 mL | 4.2756 mL | |
| 15 mM | 0.1140 mL | 0.5701 mL | 1.1401 mL | 2.8504 mL | |
| 20 mM | 0.0855 mL | 0.4276 mL | 0.8551 mL | 2.1378 mL | |
| 25 mM | 0.0684 mL | 0.3420 mL | 0.6841 mL | 1.7102 mL | |
| 30 mM | 0.0570 mL | 0.2850 mL | 0.5701 mL | 1.4252 mL | |
| 40 mM | 0.0428 mL | 0.2138 mL | 0.4276 mL | 1.0689 mL | |
| 50 mM | 0.0342 mL | 0.1710 mL | 0.3420 mL | 0.8551 mL | |
| 60 mM | 0.0285 mL | 0.1425 mL | 0.2850 mL | 0.7126 mL | |
| 80 mM | 0.0214 mL | 0.1069 mL | 0.2138 mL | 0.5344 mL | |
| 100 mM | 0.0171 mL | 0.0855 mL | 0.1710 mL | 0.4276 mL |
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