PRMT4-IN-1
PRMT4-IN-1 is a selective inhibitor of PRMT4 (IC50=3.2 nM). PRMT4-IN-1 inhibits MCF7 relative viability.
For research use only. We do not sell to patients.
- CAS No.: 3035425-70-5
- Formula: C30H36N10O7
- Molecular Weight:648.67
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
All Histone Methyltransferase Isoforms
More
Biological Activity
Description
IC50 & Target
[1]|
PRMT1 0.835 μM (IC50) |
PRMT3 4.05 μM (IC50) |
PRMT4 3.2 nM (IC50) |
PRMT5 1.46 μM (IC50) |
PRMT6 1.75 μM (IC50) |
PRMT7 1.68 μM (IC50) |
PRMT8 1.95 μM (IC50) |
In Vitro
PRMT4-IN-1 (compound 12h) (10 μM, 50 μM, 100 μM; 24 h, 72 h) shows no effect on reduction the number of metabolically active cells[1]. PRMT4-IN-1 (50 μM; 2-8 d) decreases relative cell viability time-dependently in MCF7 cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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CAS No. 3035425-70-5
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Molecular Weight 648.67
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Formula C30H36N10O7
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SMILES
O[C@@H]1[C@H](O)[C@@H](/C=C/CNC(NCCCCNC(NC2=CC3=C(O)C=C(C(OC)=O)C=C3C=C2)=O)=N)O[C@H]1N4C=NC5=C(N)N=CN=C45
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
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Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
Purity & Documentation
References
[1]. Iannelli G, et al. Turning Nonselective Inhibitors of Type I Protein Arginine Methyltransferases into Potent and Selective Inhibitors of Protein Arginine Methyltransferase 4 through a Deconstruction-Reconstruction and Fragment-Growing Approach. J Med Chem. 2022 Sep 8;65(17):11574-11606. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
PRMT4-IN-1 Related Classifications
HY-146810 Related Classifications
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