Cat. No.: HY-175341

Rac1-IN-5

Customer Review

Based on 1 Customer Validation

Rac1-IN-5 is a specific, reversible inhibitor of RAC1 (K_D = 30 nM). Rac1-IN-5 competes with guanine nucleotides for specific binding to RAC proteins, effectively blocking RAC1 activity and RAC1-dependent cellular functions. Rac1-IN-5 exhibits anti-tumor metastasis effects in vivo and can improve survival. Rac1-IN-5 can be used to study invasive cancers such as triple-negative breast cancer and colon cancer.

For research use only. We do not sell to patients.

Purity: 99.84%
CAS No.: 694515-65-6
Formula: C₂₄H₂₆N₂O₅S₂
Molecular Weight:486.60

Storage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

Rac1-IN-5 Chemical Structure

CAS No.: 694515-65-6

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- In Vitro
- Parmacokinetics
- In Vivo
Chemical Information
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

All Acyltransferase Isoforms :

All Ras Isoforms :

All Akt Isoforms :

In Vitro

Rac1-IN-5 (Compound A41) (10 μM, 1 h) blocks RAC1 activation and RAC1-dependent functions in EGF-stimulated NIH/3T3 fibroblasts^[1].
Rac1-IN-5 (10 μM) reduces the activity of RAC P29S and RAC1b in NIH-3T3 fibroblasts expressing RAC wild-type (RAC1 WT) or RAC1 oncomutants (RAC1 P29S and RAC1b)^[1].
Rac1-IN-5 (0.01-10 μM) reduces RAC1-GTP levels, the over-activation of AKT and the migration area of spheroids, inhibits colony formation (IC₅₀ = 2.1 μM) in TNBC MDA-MB-468 luciferase (Luc) cells^[1].
Rac1-IN-5 (10 μM) has anti-invasive properties in human breast CAF spheroids^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[1]

Cell Line:

MDA-MB-468 Luc cells
Concentration:

10 μM
Incubation Time:

1 h
Result:

Reduced AKT overactivation, while p44/42 activation remained unchanged.

Parmacokinetics

Species	Dose	Route	T_1/2	C₀	V_d	C_max	T_max
Mice^[1]	25 mg/kg	i.p.	97.764 min	4.335 μg/mL	5.767 mL	15.056 μg/mL	5 min

In Vivo

Rac1-IN-5 (Compound A41) (25 mg/kg, i.p., once a day, 4 weeks) prevents metastasis in a mouse model of TNBC^[1].
Rac1-IN-5 (25 mg/kg, i.p., once a day, 15 days) prevents metastasis in invasive cancers in CT26 cellsxenograft BALB/c female mice model^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

TNBC (4 million MDA-MB-468Luc cells) xenograft NMRI nude mice (5-week-old) model^[1]
Dosage:

25 mg/kg
Administration:

i.p., once a day, 4 weeks
Result:

Reduced primary tumor regeneration, secondary tumors in the femur, lungs, kidneys, ovaries, and uterus, reduced ex vivo luminescence intensity in the ovaries and uterus.

Animal Model:

TNBC (4 million MDA-MB-468Luc cells) xenograft BALB/c female mice (7-8-week-old) model^[1]
Dosage:

25 mg/kg
Administration:

i.p., once a day, 4 weeks
Result:

Reduced the frequency of primary tumor regrowth and metastasis, improving survival rates.

Animal Model:

CT26 cells (1x10⁶) xenograft BALB/c female mice (7-8-week-old) model^[1]
Dosage:

25 mg/kg
Administration:

i.p., once a day, 15 days
Result:

Reduced the development of lung metastases and liver metastases, improving survival rates.

Chemical Information

CAS No. 694515-65-6
Appearance Solid
Molecular Weight 486.60
Formula C₂₄H₂₆N₂O₅S₂
Color White to off-white
SMILES

CC1=CC=C(C=C1)SCCC(NC2=CC=C(C=C2)S(NC3=CC(OC)=CC=C3OC)(=O)=O)=O
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (205.51 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.0551 mL

10.2754 mL

20.5508 mL

5 mM

0.4110 mL

2.0551 mL

4.1102 mL

10 mM

0.2055 mL

1.0275 mL

2.0551 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 5 mg/mL (10.28 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 5 mg/mL (10.28 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (273 KB)
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Dilasser F, et al. A Rac-specific competitive inhibitor of guanine nucleotide binding reduces metastasis in triple-negative breast cancer. Cell Rep Med. 2025 Jul 15;6(7):102233. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.0551 mL	10.2754 mL	20.5508 mL	51.3769 mL
	5 mM	0.4110 mL	2.0551 mL	4.1102 mL	10.2754 mL
	10 mM	0.2055 mL	1.0275 mL	2.0551 mL	5.1377 mL
	15 mM	0.1370 mL	0.6850 mL	1.3701 mL	3.4251 mL
	20 mM	0.1028 mL	0.5138 mL	1.0275 mL	2.5688 mL
	25 mM	0.0822 mL	0.4110 mL	0.8220 mL	2.0551 mL
	30 mM	0.0685 mL	0.3425 mL	0.6850 mL	1.7126 mL
	40 mM	0.0514 mL	0.2569 mL	0.5138 mL	1.2844 mL
	50 mM	0.0411 mL	0.2055 mL	0.4110 mL	1.0275 mL
	60 mM	0.0343 mL	0.1713 mL	0.3425 mL	0.8563 mL
	80 mM	0.0257 mL	0.1284 mL	0.2569 mL	0.6422 mL
	100 mM	0.0206 mL	0.1028 mL	0.2055 mL	0.5138 mL

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Rac1-IN-5

Bioactive Compound Library Max

Bioactive Compound Library

GPCR/G Protein Compound Library

Immunology/Inflammation Compound Library

Kinase Inhibitor Library

MAPK Compound Library

Metabolism/Protease Compound Library

PI3K/Akt/mTOR Compound Library

Stem Cell Signaling Compound Library

Anti-Cancer Compound Library

Autophagy Compound Library

Anti-Aging Compound Library

Differentiation Inducing Compound Library

Oxygen Sensing Compound Library

Ferroptosis Compound Library

Glycolysis Compound Library

Cytoskeleton Compound Library

Glutamine Metabolism Compound Library

Anti-Breast Cancer Compound Library

Anti-Lung Cancer Compound Library

Anti-Pancreatic Cancer Compound Library

Anti-Blood Cancer Compound Library

Anti-Cancer Metabolism Compound Library

Anti-Obesity Compound Library

Angiogenesis-Related Compound Library

Lipid Metabolism Compound Library

Glucose Metabolism Compound Library

Anti-Liver Cancer Compound Library

Anti-Colorectal Cancer Compound Library

Anti-Prostate Cancer Compound Library

Anti-Pulmonary Fibrosis Compound Library

Cancer Stem Cells Compound Library

Pain-Related Compound Library

Metabolic Enzyme Compound Library

Membrane Protein-targeted Compound Library

Membrane Receptor-targeted Compound Library

Highly Selective Inhibitors Library

Serine/Threonine Kinase Inhibitor Library

Anti-Hematopathy Compound Library

Anti-Ovarian Cancer Compound Library

Multi-Target Compound Library

MCE Bioactive Compound Library

Anti-Gastric Cancer Compound Library

Anti-Fibrosis Compound Library

Protein Kinase Compound Library

Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

Cell Proliferation Compound Library

High-Throughput Bioactive Compound Library

Lactylation Compound Library

All Acyltransferase Isoforms

All Ras Isoforms

All Akt Isoforms

Biological Activity

Chemical Information

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Rac1-IN-5 Related Classifications

HY-175341 Related Classifications