Rhein 8-O-β-D-Glucopyranoside

Name: Rhein 8-O-β-D-Glucopyranoside
Brand: MedChemExpress
SKU: HY-N6082
Rating: 4.5 (1 reviews)

Cat. No.: HY-N6082 Purity: 99.81%: Data Sheet
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Rhein 8-O-β-D-Glucopyranoside is an orally active glycoside found in Rhubarb. Rhein 8-O-β-D-Glucopyranoside attenuates high glucose-induced apoptosis, recovers altered lincRNA ANRIL and let-7a expression, reverses high glucose-altered Bcl-2 and cleaved caspase-3 protein expression, and inhibits TGF-β1/Smad signaling. Rhein 8-O-β-D-Glucopyranoside accelerates Sennoside A (HY-N0365) metabolism, stimulates sennoside A purgative activity. Rhein 8-O-β-D-Glucopyranoside inhibits bacterial biofilm formation, suppresses its virulence gene expression, and exerts antibacterial activity. Rhein 8-O-β-D-Glucopyranoside can be used for the research of diabetic nephropathy, constipation, and infection.

For research use only. We do not sell to patients.

Rhein 8-O-β-D-Glucopyranoside Chemical Structure

CAS No. : 34298-86-7

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
1 mg	In-stock	Estimated Time of Arrival: December 31
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	Get quote
100 mg	Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of Rhein 8-O-β-D-Glucopyranoside:

Rhein 8-O-β-D-Glucopyranoside (Standard) Get quote

1 Publications Citing Use of MCE Rhein 8-O-β-D-Glucopyranoside

•Biomed Res Int. 2021 Sep 9;2021:9066938. [Abstract]

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Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target^[1]

Caspase 3

Bcl-2

In Vitro

Rhein 8-O-β-D-Glucopyranoside (20-80 μM; 48 h) attenuates high glucose-induced apoptosis in human mesangial cells^[1].
Rhein 8-O-β-D-Glucopyranoside (20-80 μM; 1-5 days) reverses high glucose-suppressed proliferation in human mesangial cells^[1].
Rhein 8-O-β-D-Glucopyranoside (20-80 μM; 48 h) normalizes high glucose-altered lincRNA ANRIL and let-7a expression in human mesangial cells^[1].
Rhein 8-O-β-D-Glucopyranoside (20-80 μM; 48 h) reverses high glucose-altered Bcl-2 and cleaved caspase-3 protein expression in human mesangial cells^[1].
Rhein 8-O-β-D-Glucopyranoside (20-80 μM; 48 h) inhibits high glucose-activated TGF-β1/Smad signaling in human mesangial cells^[1].
Rhein 8-O-β-D-Glucopyranoside (0.03-1 mM; 4 h) significantly accelerates the metabolism of Sennoside A (HY-N0365) in anaerobic mouse intestinal flora suspensions in a concentration-dependent manner^[2].
Rhein 8-O-β-D-Glucopyranoside (10-50 μg/mL; 24 h) significantly inhibits the growth of Streptococcus mutans^[3].
Rhein 8-O-β-D-Glucopyranoside (1-75 μg/mL; 4-24 h) inhibits Streptococcus mutans biofilm formation in a concentration-dependent manner, with 50 μg/mL and 75 μg/mL almost fully inhibiting biofilm formation at 4 h and 24 h, and 10 μg/mL suppressing nearly 60% of biofilm formation^[3].
Rhein 8-O-β-D-Glucopyranoside (50 μg/mL; 48 h) significantly decreases the expression of luxS, brpA, ffh, recA, nth, and smx virulence-associated genes in Streptococcus mutans biofilms^[3].
Rhein 8-O-β-D-Glucopyranoside (25-75 μg/mL; 24 h) is non-toxic to human THP monocytes after 24 h of incubation, and significantly restores THP monocyte viability reduced by Streptococcus mutans infection^[3].
Rhein 8-O-β-D-Glucopyranoside (25-75 μg/mL; 24 h) significantly reduces increased lactate dehydrogenase activity in human THP monocytes caused by S. mutans infection, confirming non-toxicity to monocytes^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Apoptosis Analysis^[1]

Cell Line:	human mesangial cells (HMCs)
Concentration:	20 μM, 80 μM
Incubation Time:	48 h
Result:	Reduced the percentage of apoptotic HMCs induced by high glucose. Reduced the apoptotic cell percentage to a level comparable to that of non-targeted control interventions (ANRIL siRNA, let-7a mimics) at 80 μM.

Real Time qPCR^[1]

Cell Line:	human mesangial cells (HMCs)
Concentration:	20 μM, 80 μM
Incubation Time:	48 h
Result:	Reduced high glucose-induced lincRNA ANRIL expression. Increased high glucose-reduced let-7a expression.

Western Blot Analysis^[1]

Cell Line:	human mesangial cells (HMCs)
Concentration:	20 μM, 80 μM
Incubation Time:	48 h
Result:	Recovered Bcl-2 expression inhibited by high glucose. Reduced cleaved caspase-3 expression increased by high glucose to levels closer to those of untreated control cells.\nReduced TGF-β1 expression upregulated by high glucose. Decreased the ratio of p-Smad2 to total Smad2 increased by high glucose. Increased Smad7 expression downregulated by high glucose to levels closer to those of untreated control cells.

Cell Viability Assay^[3]

Cell Line:	human THP monocyte cell line
Concentration:	25 μg/mL, 50 μg/mL, 75 μg/mL
Incubation Time:	24 h
Result:	Had no effect on monocyte viability at 25, 50, and 75 μg/mL alone compared to the control group. Significantly restored monocyte viability reduced by Streptococcus mutans infection.

In Vivo

Rhein 8-O-β-D-Glucopyranoside (2.1-16.8 mg/kg; p.o.; single dose) dose-dependently and significantly enhances the purgative activity of Sennoside A (HY-N0365) in male ddY mice^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	ddY (male, 30-40 g)^[2]
Dosage:	2.1 mg/kg; 4.2 mg/kg; 8.4 mg/kg; 16.8 mg/kg
Administration:	p.o.; single dose
Result:	Resulted in a mean feces score significantly higher than Sennoside A alone at 2.1 mg/kg. Resulted in a further significant increase in mean feces score at 4.2 mg/kg. Resulted in a marked significant increase in mean feces score at 8.4 mg/kg. Resulted in the greatest significant increase in mean feces score, reaching a value of ~8 at 16.8 mg/kg. Showed a dose-dependent stimulation of Sennoside A's purgative activity across all tested doses.

Molecular Weight

446.36

Formula

C₂₁H₁₈O₁₁

CAS No.

34298-86-7

Appearance

Solid

Color

Light yellow to yellow

SMILES

O=C(C1=CC(C(O)=O)=CC(O)=C1C2=O)C3=C2C(O[C@@H]4O[C@@H]([C@@H](O)[C@H](O)[C@H]4O)CO)=CC=C3

Structure Classification

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (224.03 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.2403 mL	11.2017 mL	22.4034 mL
5 mM	0.4481 mL	2.2403 mL	4.4807 mL
10 mM	0.2240 mL	1.1202 mL	2.2403 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 4.55 mg/mL (10.19 mM); Clear solution

This protocol yields a clear solution of ≥ 4.55 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (45.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (5.60 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.81%

Select Batch:

Data Sheet (288 KB) SDS (393 KB)

COA (257 KB) LCMS (95 KB)

Handling Instructions (2659 KB)

References

[1]. Zhang L S, et al. Rhein-8-O-β-D-glucopyranoside inhibited high glucose-induced apoptosis of human mesangial cells by regulating the lincRNA ANRIL/let-7a/TGF-β1/Smad signaling pathway[J]. Experimental and therapeutic medicine, 2020, 19(4): 2871-2878. [Content Brief]

[2]. Takayama K, et al. The influence of rhein 8-O-β-D-glucopyranoside on the purgative action of sennoside A from rhubarb in mice. Biol Pharm Bull. 2012;35(12):2204-2208. [Content Brief]

[3]. Zhang Y, et al. Effects of Rhein-8-O-β-D-glucopyranoside on the biofilm formation of Streptococcus mutans[J]. Current Microbiology, 2021, 78(1): 323-328. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.2403 mL	11.2017 mL	22.4034 mL	56.0086 mL
	5 mM	0.4481 mL	2.2403 mL	4.4807 mL	11.2017 mL
	10 mM	0.2240 mL	1.1202 mL	2.2403 mL	5.6009 mL
	15 mM	0.1494 mL	0.7468 mL	1.4936 mL	3.7339 mL
	20 mM	0.1120 mL	0.5601 mL	1.1202 mL	2.8004 mL
	25 mM	0.0896 mL	0.4481 mL	0.8961 mL	2.2403 mL
	30 mM	0.0747 mL	0.3734 mL	0.7468 mL	1.8670 mL
	40 mM	0.0560 mL	0.2800 mL	0.5601 mL	1.4002 mL
	50 mM	0.0448 mL	0.2240 mL	0.4481 mL	1.1202 mL
	60 mM	0.0373 mL	0.1867 mL	0.3734 mL	0.9335 mL
	80 mM	0.0280 mL	0.1400 mL	0.2800 mL	0.7001 mL
	100 mM	0.0224 mL	0.1120 mL	0.2240 mL	0.5601 mL