Ethylvanillin
Based on 1 Customer Validation
Ethylvanillin is an orally active antioxidant that can affect cytochrome P450 activity in rats. It can also be used as a food flavoring agent in the field of food processing.
For research use only. We do not sell to patients.
- Purity: 99.88%
- CAS No.: 121-32-4
- Formula: C9H10O3
- Molecular Weight:166.18
-
Storage:
4°C, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
-
Solvent & Solubility
- Purity & Documentation
- References
-
Help & FAQs
Biological Activity
Ethylvanillin (25 mM, 180 min) inhibits AAPH-induced hemolysis in the erythrocyte suspension in erythrocyte oxidative assay[1]. Ethylvanillin (20 mM, 2 hours) shows antioxidant activity in the erythrocyte suspension in ABTS radical assay[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Animal Model:ICR mice (6 weeks)[1]
-
Dosage:32.7 mg/kg
-
Administration:Oral gavage (p.o.), single dose
-
Result:Increased ethyl vanillic acid metabolite concentration in plasma; Raised antioxidant activity in plasma.
-
Animal Model:Wistar rats (6 weeks, 180-200 g)[1]
-
Dosage:3, 15, 75 mg/kg
-
Administration:Oral gavage (p.o.), once daily for 7 days
-
Result:Increased CYP2E1 activity, decreased CYP1A2 activity.
Chemical Information
-
CAS No. 121-32-4
-
Appearance Solid
-
Molecular Weight 166.18
-
Formula C9H10O3
-
Color White to off-white
-
SMILES
O=CC1=CC=C(O)C(OCC)=C1
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
4°C, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
Solvent & Solubility
Ethanol : ≥ 100 mg/mL (601.76 mM)
DMSO : ≥ 100 mg/mL (601.76 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 5 mg/mL (30.09 mM; ultrasonic and warming and heat to 60°C)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (15.04 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (15.04 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
-
-
-
-
Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
-
%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
-
%+
-
+%Tween-80 + +
-
%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
-
Data Sheet (286 KB)
-
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
-
Handling Instructions (2659 KB)
References
[1]. Tai A, et al. Antioxidant properties of ethyl vanillin in vitro and in vivo. Biosci Biotechnol Biochem. 2011;75(12):2346-50. [Content Brief]
[2]. Chen XM, et al. Effect of vanillin and ethyl vanillin on cytochrome P450 activity in vitro and in vivo. Food Chem Toxicol. 2012 Jun;50(6):1897-901. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O / Ethanol / DMSO | 1 mM | 6.0176 mL | 30.0879 mL | 60.1757 mL | 150.4393 mL |
| 5 mM | 1.2035 mL | 6.0176 mL | 12.0351 mL | 30.0879 mL | |
| 10 mM | 0.6018 mL | 3.0088 mL | 6.0176 mL | 15.0439 mL | |
| 15 mM | 0.4012 mL | 2.0059 mL | 4.0117 mL | 10.0293 mL | |
| 20 mM | 0.3009 mL | 1.5044 mL | 3.0088 mL | 7.5220 mL | |
| 25 mM | 0.2407 mL | 1.2035 mL | 2.4070 mL | 6.0176 mL | |
| 30 mM | 0.2006 mL | 1.0029 mL | 2.0059 mL | 5.0146 mL | |
| Ethanol / DMSO | 40 mM | 0.1504 mL | 0.7522 mL | 1.5044 mL | 3.7610 mL |
| 50 mM | 0.1204 mL | 0.6018 mL | 1.2035 mL | 3.0088 mL | |
| 60 mM | 0.1003 mL | 0.5015 mL | 1.0029 mL | 2.5073 mL | |
| 80 mM | 0.0752 mL | 0.3761 mL | 0.7522 mL | 1.8805 mL | |
| 100 mM | 0.0602 mL | 0.3009 mL | 0.6018 mL | 1.5044 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Ethylvanillin Related Classifications
HY-B0940 Related Classifications
Powered by Bioz