Cat. No.: HY-125731

Glycodeoxycholic acid

5 Cited Publications

Customer Review

Based on 5 publication(s) in Google Scholar

Glycodeoxycholic Acid is a natural product found in Streptomyces nigricans, Trypanosoma brucei and C. elegans. Glycodeoxycholic Acid induces hepatocyte necrosis and autophagy in patients with obstructive cholestasis.

For research use only. We do not sell to patients.

Purity: 99.91%
CAS No.: 360-65-6
Formula: C₂₆H₄₃NO₅
Molecular Weight:449.62

Storage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year

Glycodeoxycholic acid Chemical Structure

CAS No.: 360-65-6

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (5)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

All Endogenous Metabolite Isoforms :

All STAT Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
CHO	EC₅₀	1.18 μM Compound: 7b, glyco	Agonist activity at human TGR5 expressed in CHO cells by luciferase assay Agonist activity at human TGR5 expressed in CHO cells by luciferase assay	[PMID: 18307294]
HET-1A	CC₅₀	1237 μM Compound: 12, TDCA	Cytotoxicity against human HET-1A cells assessed as cell viability after 24 hrs by MTT assay Cytotoxicity against human HET-1A cells assessed as cell viability after 24 hrs by MTT assay	[PMID: 20713311]

In Vitro

Glycodeoxycholic Acid (200 μM, 24-48 h) induces stemness and chemotherapy resistance of hepatocellular carcinoma cells through STAT3 signaling pathway^[1].
Glycodeoxycholic Acid (50 μM, pretreatment for 1 h) can eliminate UCB-induced cytochrome c oxidase inhibition, and significantly prevent oxidative stress, metabolic changes and cell death^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:

Huh7, LM3
Concentration:

200 μM
Incubation Time:

24, 48 h
Result:

Increased cell viability treated with 5-FU and cisplatin.

Western Blot Analysis^[1]

Cell Line:

Huh7, LM3
Concentration:

200 μM
Incubation Time:

24, 48 h
Result:

Suppressed the expression of apoptotic genes and increased anti-apoptotic genes.
Promoted the expression of Sox2, Sox9, Nanog and CD133.
Down-regulated the level of E-cadherin and up-regulated vimentin.
Decreased the levels of SOCS2, SOCS5, PTPN1 and PTPN11.

In Vivo

Glycodeoxycholic Acid (11.20 mg/kg, biliary and pancreatic duct injection) can induce acute pancreatitis in rhesus monkeys^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Experimental macaque model^[3]
Dosage:

11.20 mg/kg
Administration:

injected along the biliopancreatic duct
Result:

Increased the levels of Serum amylase and lipase.
Elevated Blood pressure and heart rate.

Chemical Information

CAS No. 360-65-6
Appearance Solid
Molecular Weight 449.62
Formula C₂₆H₄₃NO₅
Color White to off-white
SMILES

C[C@@H]([C@]1(CC[C@]2([C@@]3(CC[C@@]4(C[C@@H](CC[C@@]4([C@]3(C[C@@H]([C@@]21C)O)[H])C)O)[H])[H])[H])[H])CCC(NCC(O)=O)=O
Structure Classification
- Steroids
Initial Source
- Endogenous metabolite
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Publications (5)

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Journal Impact Factor

Nature

Lithocholic acid phenocopies anti-ageing effects of calorie restriction. [Abstract]

2025 Jul;643(8070):192-200. PMID: 39695227
Adv Sci (Weinh)

Targeting FDFT1 Reduces Cholesterol and Bile Acid Production and Delays Hepatocellular Carcinoma Progression Through the HNF4A/ALDOB/AKT1 Axis. [Abstract]

2025 Feb 3:e2411719. PMID: 39899681

Glycodeoxycholic acid purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Feb 3:e2411719. [Abstract]

The mRNA levels of ALDOB in Huh7 or HCCLM3 cells treated with various bile acids (100 μM, 24 h). All data are presented as mean ± SD. Data were analyzed by one-way ANOVA with Bonferroni multiple-comparison correction. CA, cholic acid; TCA, taurocholic acid; GCA, glycocholic acid; TCDCA, taurochenodeoxycholic acid; GCDCA, glycochenodeoxycholic acid; LCA, lithocholic acid; TLCA, taurolithocholic acid; GLCA, glycolithocholic acid; DCA, deoxycholic acid; TDCA, taurodeoxycholic acid; GDCA, glycodeoxycholic acid; UDCA, ursodeoxycholic acid; TUDCA, tauroursodeoxycholic acid; GUDCA, glycoursodeoxycholic acid.
J Enzyme Inhib Med Chem

Natural product sennoside B disrupts liquid-liquid phase separation of SARS-CoV-2 nucleocapsid protein by inhibiting its RNA-binding activity. [Abstract]

2025 Dec;40(1):2501743. PMID: 40371698
Metabolomics

2026 Jun 18;22(4):103.
bioRxiv

Bile acid chemosensation in mammals supports species and gut microbiome evaluation. [Abstract]

2026 May 5:2026.04.30.721772. PMID: 42146479

Solvent & Solubility

In Vitro:

DMSO : 87.5 mg/mL (194.61 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.2241 mL

11.1205 mL

22.2410 mL

5 mM

0.4448 mL

2.2241 mL

4.4482 mL

10 mM

0.2224 mL

1.1121 mL

2.2241 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.08 mg/mL (4.63 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.08 mg/mL (4.63 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.08 mg/mL (4.63 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (278 KB)
SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Shi C, et al. Glycochenodeoxycholic acid induces stemness and chemoresistance via the STAT3 signaling pathway in hepatocellular carcinoma cells. Aging (Albany NY). 2020 Aug 3;12(15):15546-15555. [Content Brief]

[2]. Vaz AR, et al. Bilirubin selectively inhibits cytochrome c oxidase activity and induces apoptosis in immature cortical neurons: assessment of the protective effects of glycoursodeoxycholic acid. J Neurochem. 2010 Jan;112(1):56-65. [Content Brief]

[3]. Fauzi A, et al. Role of glycodeoxycholic acid to induce acute pancreatitis in Macaca nemestrina. J Med Primatol. 2022 Jun;51(3):134-142. doi: 10.1111/jmp.12577. Epub 2022 Mar 20. PMID: 35306662; PMCID: PMC9310849. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.2241 mL	11.1205 mL	22.2410 mL	55.6025 mL
	5 mM	0.4448 mL	2.2241 mL	4.4482 mL	11.1205 mL
	10 mM	0.2224 mL	1.1121 mL	2.2241 mL	5.5603 mL
	15 mM	0.1483 mL	0.7414 mL	1.4827 mL	3.7068 mL
	20 mM	0.1112 mL	0.5560 mL	1.1121 mL	2.7801 mL
	25 mM	0.0890 mL	0.4448 mL	0.8896 mL	2.2241 mL
	30 mM	0.0741 mL	0.3707 mL	0.7414 mL	1.8534 mL
	40 mM	0.0556 mL	0.2780 mL	0.5560 mL	1.3901 mL
	50 mM	0.0445 mL	0.2224 mL	0.4448 mL	1.1121 mL
	60 mM	0.0371 mL	0.1853 mL	0.3707 mL	0.9267 mL
	80 mM	0.0278 mL	0.1390 mL	0.2780 mL	0.6950 mL
	100 mM	0.0222 mL	0.1112 mL	0.2224 mL	0.5560 mL

Keywords

Glycodeoxycholic acid

5 Cited Publications

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Glycodeoxycholic acid

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Publications Citing Use of MedChemExpress (MCE) Glycodeoxycholic acid

All Endogenous Metabolite Isoforms

All STAT Isoforms

Biological Activity

Chemical Information

Publications (5)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Glycodeoxycholic acid Related Classifications

HY-125731 Related Classifications