STX-100 

STX-100 is a humanized monoclonal antibody targeting αVβ6 integrin. STX-100 specifically binds αVβ6 integrin, blocks latent TGF-β activation via latency-associated peptide (LAP) interaction prevention, and reduces profibrotic responses. STX-100 can be used for the research of idiopathic pulmonary fibrosis, and cancer^[1][2][3][4].

Human IgG1 kappa

Human IgG1 kappa, Isotype Control

Human

STX-100 (1 h pretreatment) exhibits minimal inhibitory effects on TGF-β1-induced morphological and nuclear changes in A549 cells^[3].
STX-100 (1 h pretreatment) does not substantially alter actin cytoskeletal organization in A549 cells and does not prevent TGF-β1-associated changes in actin distribution^[3].
STX-100 (1 h pretreatment) has limited inhibitory effects on TGF-β1-induced morphological and nuclear changes in MCF10A cells^[3].
STX-100 (1 h pretreatment) does not reverse TGF-β1-induced changes in cell organization, cell-cell adhesion, nuclear morphology, or extracellular matrix structure in MCF10A cells^[3].
STX-100 (1 h pretreatment) does not substantially alter TGF-β1-driven changes in fibronectin fibril organization in MCF10A cells^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

STX-100 (3 mg/kg; s.c.; weekly; 8 weeks) promotes acute renal allograft rejection in rhesus macaques^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

ELISA, FACS, Functional assay

Unconjugated

The product can be reconstituted/diluted with sterile PBS or saline.

144.88 kDa

1439902-67-6

Liquid

Colorless to light yellow

Shipping with dry ice.

Please refer to the lot-specific COA for specific buffer information.

Please store the product under the recommended conditions in the Certificate of Analysis.

• 
  Immobilized Human Integrin alpha V beta 6 (ITGAV&ITGB6) can bind STX-100. The EC₅₀ for this effect is 131.5 ng/mL.
• 
  Flow cytometric analysis of 1X10⁶ MCF-7 cells with STX-100 (HY-P990667, red). Cells were fixed with 4% paraformaldehyde. Then stained with the primary antibody at 1/200 dilution for an hour at 4℃. Alexa Fluor 488-conjugated AffiniPure Goat Anti-Human IgG H&L (AF488) (HY-P83776) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Human IgG1 kappa (HY-P99001, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

• [1]. Crunkhorn S. Deal watch: Biogen acquires Stromedix to pursue novel fibrosis therapy. Nat Rev Drug Discov. 2012 Mar 30;11(4):260.

  [2]. Lo DJ, et al. Inhibition of αvβ6 promotes acute renal allograft rejection in nonhuman primates. Am J Transplant. 2013;13(12):3085-3093.

  [3]. Wesam Elhawabri. REGULATION OF TGF-β1–INDUCED EPITHELIAL–MESENCHYMAL TRANSITION BY INTEGRIN INHIBITION.

  [4]. Sylvers PH, et al. Mitigation of harmful algal blooms caused by Alexandrium catenella and reduction in saxitoxin accumulation in bivalves using cultivable seaweeds. Harmful Algae. 2021;105:102056.