α5β1 integrin agonist-2
Based on 1 Customer Validation
α5β1 integrin agonist-2 (Compound 2) is a selective α5β1 integrin agonist (EC50 = 45.98 nM). α5β1 integrin agonist-2 induces integrin activation. α5β1 integrin agonist-2 is applicable to the research of leukemia.
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- Pureza: 99.80%
- No. CAS: 3115530-47-4
- Fòrmula: C13H13FN2O4
- Peso molecular:280.25
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Almacenamiento:
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
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Actividad biológica
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α5β1 45.98 nM (EC50) |
α5β1 integrin agonist-2 (10-10-10-4 M) is a selective and potent agonist of α5β1 integrin in PMA-differentiated leukemic K562 cells, with an EC50 of 45.98 nM[1].
α5β1 integrin agonist-2 (10-11-10-5 M; 30 min) does not bind to α4β1 integrin in Jurkat E6.1 acute T-cell leukemia cells, with a Ki > 5000 nM[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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No. CAS 3115530-47-4
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Appearance Solid
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Peso molecular 280.25
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Fòrmula C13H13FN2O4
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Color White to off-white
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SMILES
O=C(O)CC(C1)N(C(NC2=CC(F)=CC=C2C)=O)C1=O
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvente y solubilidad
DMSO : 100 mg/mL (356.82 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2 mg/mL (7.14 mM); Clear solution
This protocol yields a clear solution of ≥ 2 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureza y Documentación
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Ficha de datos (276 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
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- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Instrucciones de manejo (2659 KB)
Referencias
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.5682 mL | 17.8412 mL | 35.6824 mL | 89.2061 mL |
| 5 mM | 0.7136 mL | 3.5682 mL | 7.1365 mL | 17.8412 mL | |
| 10 mM | 0.3568 mL | 1.7841 mL | 3.5682 mL | 8.9206 mL | |
| 15 mM | 0.2379 mL | 1.1894 mL | 2.3788 mL | 5.9471 mL | |
| 20 mM | 0.1784 mL | 0.8921 mL | 1.7841 mL | 4.4603 mL | |
| 25 mM | 0.1427 mL | 0.7136 mL | 1.4273 mL | 3.5682 mL | |
| 30 mM | 0.1189 mL | 0.5947 mL | 1.1894 mL | 2.9735 mL | |
| 40 mM | 0.0892 mL | 0.4460 mL | 0.8921 mL | 2.2302 mL | |
| 50 mM | 0.0714 mL | 0.3568 mL | 0.7136 mL | 1.7841 mL | |
| 60 mM | 0.0595 mL | 0.2974 mL | 0.5947 mL | 1.4868 mL | |
| 80 mM | 0.0446 mL | 0.2230 mL | 0.4460 mL | 1.1151 mL | |
| 100 mM | 0.0357 mL | 0.1784 mL | 0.3568 mL | 0.8921 mL |
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