Cat. No.: HY-Y0335

1,3-Dihydroxyacetone

(Synonyms: Dihydroxyacetone)

1 Cited Publications

Customer Review

Based on 1 publication(s) in Google Scholar

1,3-Dihydroxyacetone (DHA), the main active ingredient in sunless tanning skin-care preparations and an important precursor for the synthesis of various fine chemicals, is produced on an industrial scale by microbial fermentation of glycerol (HY-B1659) in Gluconobacter oxydans. 1,3-Dihydroxyacetone is also used for synthesis of new biodegradable polymers by combining with lactic acid (HY-B2227).

For research use only. We do not sell to patients.

Purity: 98.0%
CAS No.: 96-26-4
Formula: C₃H₆O₃
Molecular Weight:90.08

Storage:

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

1,3-Dihydroxyacetone Chemical Structure

CAS No.: 96-26-4

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Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
- Clinical Trial
Chemical Information
Publications (1)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target

Microbial Metabolite

All Antibiotic Isoforms :

In Vitro

DHA (20-30 g/L) can inhibit the mitotic activity of the gram-negative bacterium G. oxydans^[3].
At higher concentrations of 1,3-Dihydroxyacetone (70 g/L), it completely inhibits G. oxydans cells, blocking the fermentation process and thereby affecting the production of 1,3-Dihydroxyacetone^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

NCT Number	Sponsor	Condition	Start Date	Phase A549
NCT01329991	Plexxikon\|		2011-05	PHASE1

Chemical Information

CAS No. 96-26-4
Appearance Solid
Molecular Weight 90.08
Formula C₃H₆O₃
Color White to off-white
SMILES

O=C(CO)CO
Synonyms

Dihydroxyacetone
Structure Classification
- Ketones, Aldehydes, Acids
Initial Source
- Endogenous metabolite
- Human Gut Microbiota Metabolites
- Microorganisms
Gluconobacter oxydans
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Publications (1)

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Journal Impact Factor

J Nanobiotechnology

Dihydroxyacetone nanoparticles ameliorate osteoarthritis via P21-mediated regulation of chondrocyte repair. [Abstract]

2026 May 26. PMID: 42192449

Solvent & Solubility

In Vitro:

H₂O : 116.67 mg/mL (1295.18 mM; Need ultrasonic)

DMSO : 100 mg/mL (1110.12 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

11.1012 mL

55.5062 mL

111.0124 mL

5 mM

2.2202 mL

11.1012 mL

22.2025 mL

10 mM

1.1101 mL

5.5506 mL

11.1012 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

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Volume _(start) Volume _(start)

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Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (27.75 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (27.75 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (27.75 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Working solution concentration: 0.22 mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact

MedChemExpress (MCE) .

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (271 KB)
SDS (254 KB)
- English - EN (254 KB)
- Français - FR (254 KB)
- Deutsch - DE (254 KB)
- Norwegian - NO (254 KB)
- Español - ES (254 KB)
- Swedish - SV (254 KB)
- Italian - IT (254 KB)
- Korean - KR (254 KB)
- Portuguese - PT (254 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Zheng Z, et al. Novel Process for 1,3-Dihydroxyacetone Production from Glycerol. 1. Technological Feasibility Study and Process Design. Ind. Eng. Chem. Res. 2012, 51, 9, 3715–3721.

[2]. Dikshit PK, et al. Optimization of 1,3-dihydroxyacetone production from crude glycerol by immobilized Gluconobacter oxydans MTCC 904. Bioresour Technol. 2016 Sep;216:1058-65. [Content Brief]

[3]. Dikshit PK, et al. Process optimization and analysis of product inhibition kinetics of crude glycerol fermentation for 1,3-Dihydroxyacetone production. Bioresour Technol. 2017 Nov;244(Pt 1):362-370. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO / H₂O	1 mM	11.1012 mL	55.5062 mL	111.0124 mL	277.5311 mL
	5 mM	2.2202 mL	11.1012 mL	22.2025 mL	55.5062 mL
	10 mM	1.1101 mL	5.5506 mL	11.1012 mL	27.7531 mL
	15 mM	0.7401 mL	3.7004 mL	7.4008 mL	18.5021 mL
	20 mM	0.5551 mL	2.7753 mL	5.5506 mL	13.8766 mL
	25 mM	0.4440 mL	2.2202 mL	4.4405 mL	11.1012 mL
	30 mM	0.3700 mL	1.8502 mL	3.7004 mL	9.2510 mL
	40 mM	0.2775 mL	1.3877 mL	2.7753 mL	6.9383 mL
	50 mM	0.2220 mL	1.1101 mL	2.2202 mL	5.5506 mL
	60 mM	0.1850 mL	0.9251 mL	1.8502 mL	4.6255 mL
	80 mM	0.1388 mL	0.6938 mL	1.3877 mL	3.4691 mL
	100 mM	0.1110 mL	0.5551 mL	1.1101 mL	2.7753 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Keywords

1,3-Dihydroxyacetone

1 Cited Publications

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1,3-Dihydroxyacetone

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Publications Citing Use of MedChemExpress (MCE) 1,3-Dihydroxyacetone

All Antibiotic Isoforms

Biological Activity

Chemical Information

Publications (1)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

1,3-Dihydroxyacetone Related Classifications

HY-Y0335 Related Classifications