12(S)-HETE-d8
12(S)-HETE-d8 contains eight deuterium atoms at the 5, 6, 8, 9, 11, 12, 14 and 15 positions. 12(S)-HETE-d8 is intended for use as an internal standard for the quantification of 12(S)-HETE by GC- or LC-mass spectrometry (MS). 12(S)-HETE-d8 is the predominant lipoxygenase product of mammalian platelets. It enhances tumor cell adhesion to endothelial cells, fibronectin, and the subendothelial matrix at 0.1 µM.
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- CAS No.: 84807-90-9
- Formule: C20H24D8O3
- Masse moléculaire:328.52
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Stockage:
Solution, -20°C, 2 years
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Activité biologique
Description
Application
1. This compound can be used as a tracer
2. This compound can be used as an internal standard for quantitative analysis by NMR, GC-MS, or LC-MS.
Chemical Information
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CAS No. 84807-90-9
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Unlabeled CAS 54397-83-0
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Appearance Liquid
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Masse moléculaire 328.52
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Formule C20H24D8O3
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Color Colorless to light yellow
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SMILES
CCCCC/C([2H])=C([2H])\C[C@]([2H])(O)/C([2H])=C/C([2H])=C([2H])\C/C([2H])=C([2H])\CCCC(O)=O
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Solution, -20°C, 2 years
Protocole
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Endothelial Tube Formation Assay
Endothelial tube formation assay evaluates the ability of endothelial cells to attach, migrate, align, and organize into capillary-like networks when cultured on gelled basement membrane extract or Matrigel; the readout is the morphology and quantity of tube-like networks, which reflects an in vitro endothelial morphogenesis step related to angiogenesis. Basement membrane extract/Matrigel provides laminin-rich extracellular matrix cues that support endothelial differentiation into capillary-like structures, but it can contain biologically active growth factors, so growth-factor-reduced matrix is preferred when testing defined angiogenic stimulators or inhibitors.
Pureté et documentation
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Fiche technique (252 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Instruction de manipulation (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)