12(S)-HETE
Based on 1 Customer Validation
12(S)-HETE is a metabolite of Arachidonic acid, AA (HY-109590). 12(S)-HETE promotes ERK and P38 MAPK phosphorylation, stimulates DNA synthesis and cell proliferation in cancer cells. 12(S)-HETE facilitates wound healing of injured murine-lung-derived microvascular endothelial cell monolayers. 12(S)-HETE can be used for the research of pancreatic cancer.
For research use only. We do not sell to patients.
- Purity: 99.10%
- CAS No.: 54397-83-0
- Formula: C20H32O3
- Molecular Weight:320.47
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Storage:
Solution, -20°C, 2 years
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
12(S)-HETE (1-100 nM; 2 h) concentration-dependently stimulates DNA synthesis in PANC-1 and HPAF human pancreatic cancer cells[1].
12(S)-HETE (24-96 h) time-dependently stimulates proliferation in PANC-1 and HPAF human pancreatic cancer cells[1].
12(S)-HETE (100 nM; 1-60 min) time-dependently induces tyrosine phosphorylation of multiple cellular proteins in PANC-1 and HPAF human pancreatic cancer cells[1].
12(S)-HETE (100 nM; 1 min-24 h) time-dependently activates ERK1/2 phosphorylation in PANC-1 and HPAF human pancreatic cancer cells[1].
12(S)-HETE (100 nM; 1-60 min) time-dependently activates P38 MAPK phosphorylation in PANC-1 and HPAF human pancreatic cancer cells, though this activation does not contribute to its mitogenic effect[1].
12(S)-HETE (100 nM; 1-60 min) does not induce JNK/SAPK phosphorylation in PANC-1 and HPAF human pancreatic cancer cells[1].
12(S)-HETE (0.1 μM; 8 days) promotes accelerated wound healing of injured CD clone 4 murine-lung-derived microvascular endothelial cell monolayers[2].
12(S)-HETE (0.01-10 μM; 1-4 days) dose-dependently increases proliferation of CD clone 4 murine-lung-derived microvascular endothelial cells[2].
12(S)-HETE (0.1 μM; 24 h) increases DNA synthesis in growth-arrested CD clone 4 murine-lung-derived microvascular endothelial cells by more than 4 fold[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:PANC-1, HPAF
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Concentration:100 nM
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Incubation Time:1 min, 5 min, 15 min, 30 min, 60 min
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Result:Stimulated tyrosine phosphorylation of multiple cellular proteins of varying molecular sizes in a time-dependent manner, with strong phosphorylation evident within 1 min and maintained throughout 60 min.
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Cell Line:PANC-1, HPAF
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Concentration:100 nM
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Incubation Time:1 min, 5 min, 10 min, 15 min, 30 min, 60 min, 3 h, 6 h, 24 h
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Result:Induced time-dependent activation of ERK1/2 in PANC-1 and HPAF cells.
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Cell Line:murine-lung-derived microvascular endothelial cells (CD clone 4)
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Concentration:0.001, 0.01, 0.1,1, 10 μM
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Incubation Time:1-4 days
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Result:Promoted CD clone 4 cell growth in a dose-dependent manner.
Chemical Information
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CAS No. 54397-83-0
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Appearance Liquid
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Molecular Weight 320.47
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Formula C20H32O3
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Color Colorless to light yellow
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SMILES
CCCCC/C=C\C[C@H](O)/C=C/C=C\C/C=C\CCCC(O)=O
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Solution, -20°C, 2 years
Purity & Documentation
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Data Sheet (266 KB)
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SDS (604 KB)
- English - EN (604 KB)
- Français - FR (604 KB)
- Deutsch - DE (604 KB)
- Norwegian - NO (604 KB)
- Español - ES (604 KB)
- Swedish - SV (604 KB)
- Italian - IT (604 KB)
- Korean - KR (604 KB)
- Portuguese - PT (604 KB)
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Handling Instructions (2659 KB)
References
[1]. Ding XZ, et al. 12-lipoxygenase metabolite 12(S)-HETE stimulates human pancreatic cancer cell proliferation via protein tyrosine phosphorylation and ERK activation. Int J Cancer. 2001;94(5):630-636. [Content Brief]
[2]. Tang DG, et al. 12(S)-HETE is a mitogenic factor for microvascular endothelial cells: its potential role in angiogenesis. Biochem Biophys Res Commun. 1995;211(2):462-468. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
12(S)-HETE Related Classifications
HY-124404A Related Classifications
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