2. PI3K/Akt/mTOR Apoptosis Metabolic Enzyme/Protease Immunology/Inflammation NF-κB Cell Cycle/DNA Damage GPCR/G Protein MAPK/ERK Pathway Protein Tyrosine Kinase/RTK
  3. Akt mTOR Apoptosis Reactive Oxygen Species (ROS) CDK Ras HSP VEGFR TNF Receptor Interleukin Related
  4. 19-epi-Scholaricine

19-epi-Scholaricine is an orally active indole alkaloid. 19-epi-Scholaricine downregulates the expression of profibrotic/apoptotic proteins (HRAS, HSP90AA1, KDR) and upregulates the expression of cell cycle-related protein (CDK2). 19-epi-Scholaricine suppresses ROS production and reduces the release of inflammatory mediators, thereby attenuating podocyte apoptosis, renal inflammation and oxidative stress by inhibiting AKT/mTOR. 19-epi-Scholaricine can be used in the research of chronic glomerulonephritis and membranous nephropathy.

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19-epi-Scholaricine

19-epi-Scholaricine Chemical Structure

CAS No. : 132923-06-9

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19-epi-Scholaricine Related Antibodies

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Description

19-epi-Scholaricine is an orally active indole alkaloid. 19-epi-Scholaricine downregulates the expression of profibrotic/apoptotic proteins (HRAS, HSP90AA1, KDR) and upregulates the expression of cell cycle-related protein (CDK2). 19-epi-Scholaricine suppresses ROS production and reduces the release of inflammatory mediators, thereby attenuating podocyte apoptosis, renal inflammation and oxidative stress by inhibiting AKT/mTOR. 19-epi-Scholaricine can be used in the research of chronic glomerulonephritis and membranous nephropathy[1][2].

In Vitro

19-epi-Scholaricine (10-50 μM; 24 h) protects ADR-stimulated MPC5 cells from injury, and significantly increases cell viability after 24 h of treatment at the concentration of 50 μM[1].
19-epi-Scholaricine (50 μM; 24 h) regulates the expression of CGN-related targets in ADR-induced MPC5 cells, downregulating HRAS, HSP90AA1 and KDR while upregulating CDK2[1].
19-epi-Scholaricine (50 μM; 24 h) significantly reduces the level of ROS production in ADR-stimulated MPC5 cells[1].
19-epi-Scholaricine (1-20 μM; 25 h) inhibits the release of NO in LPS (HY-D1056)-induced mouse podocyte MPC5 cells[2].
19-epi-Scholaricine (1 μM; 25 h) downregulates the gene expression of Tnf-α and Il-6 in LPS-induced mouse podocyte cell line MPC5[2].
19-epi-Scholaricine (1 μM; 25 h) upregulates the expression of circadian rhythm and AKT/mTOR pathway genes in LPS-stimulated mouse podocyte cell line MPC5[2].
19-epi-Scholaricine (1 μM; 24 h) reduces lipopolysaccharide (LPS)-induced reactive oxygen species (ROS) production in the mouse podocyte cell line MPC5[2].
19-epi-Scholaricine (1 μM; 24 h) restores the mitochondrial membrane potential of LPS-stimulated mouse podocytes MPC5[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

19-epi-Scholaricine Related Antibodies

Cell Viability Assay[1]

Cell Line: adriamycin (ADR)-stimulated murine glomerular podocyte (MPC5) cells
Concentration: 10, 20, 40 and 50 μM
Incubation Time: 24 h
Result: Increased the survival rate of ADR-stimulated MPC5 cells; the 50 μM concentration produced a statistically significant increase relative to the ADR-only model group.

Western Blot Analysis[1]

Cell Line: adriamycin (ADR)-stimulated murine glomerular podocyte (MPC5) cells
Concentration: 50 μM
Incubation Time: 24 h
Result: Downregulated the protein expression of HRAS, HSP90AA1, and KDR, and upregulated the protein expression of CDK2 in ADR-stimulated MPC5 cells, reversing the expression changes induced by ADR.

Real Time qPCR[1]

Cell Line: adriamycin (ADR)-stimulated murine glomerular podocyte (MPC5) cells
Concentration: 50 μM
Incubation Time: 24 h
Result: Downregulated the mRNA expression of hras, hsp90aa1, and kdr and upregulated the mRNA expression of cdk2 in ADR-stimulated MPC5 cells, reversing the expression changes induced by ADR.

Real Time qPCR[2]

Cell Line: MPC5 murine podocytes (LPS-stimulated)
Concentration: 1 μM
Incubation Time: 1 h pre-incubation, then 24 h LPS stimulation
Result: Significantly reduced the LPS-induced expression of Tnf-α and IL-6 genes in MPC5 cells, restoring levels to near normal.\nUpregulated the expression of Arntl, Cry1, Akt, mTor, and Bcl-2/Bax genes in LPS-stimulated MPC5 cells.
In Vivo

19-epi-Scholaricine (1 mg/kg; p.o.; daily; 28 days) significantly alleviates adriamycin-induced chronic glomerulonephritis in male ICR mice by reducing urinary protein, normalizing serum renal function biomarkers, and improving kidney histopathology[1].
19-epi-scholaricine (1.0 mg/kg; oral gavage; daily; 6 weeks) exhibits superior or comparable therapeutic efficacy to prednisone at 2 mg/kg in a mouse model of membranous nephropathy, as evidenced by reduced urinary protein, restored renal function, suppressed inflammation and oxidative stress, improved histopathology, and modulation of the ARNTL/AKT/mTOR pathway[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: ICR mice (male, 32-36 g, adriamycin-induced)[1]
Dosage: 1 mg/kg
Administration: p.o.; daily; 28 days
Result: Reduced urinary protein levels from 11.51 mg/24 h (model group) to 8.84 mg/24 h at day 7.
Attenuated the ADR-induced decrease in serum albumin (ALB) from 30.74 U/L (model group) to 35.92 U/L, and in serum total protein (TP) from 51.01 U/L (model group) to 55.63 U/L.
Reduced serum urea nitrogen (BUN) levels from 9.49 μmol/L (model group) to 8.44 μmol/L.
Ameliorated kidney histopathological damage, including reduced diffuse proliferation of glomerular tracts and decreased lymphocyte/plasma cell infiltration.
Animal Model: ICR (male, 25 ± 3 g, membranous nephropathy induced by 5 mg/kg C-BSA tail vein injection every 3 days for 6 doses)[2]
Dosage: 1.0 mg/kg
Administration: oral gavage; daily; 6 weeks
Result: Reduced 24-hour urinary protein from 9.9 to 6.2 mg.
Increased the urine creatinine (Ucr)/blood creatinine (Scr) ratio from 42.7 to 76.5.
Decreased renal C3 deposition integrated fluorescence signal from 2.1 to 0.52.
Reduced renal IL-1β expression from 4.4 to 1.4.
Normalized oxidative stress markers (malondialdehyde, glutathione, superoxide dismutase, catalase) in kidney homogenates.
Ameliorated histopathological renal lesions including lymphocyte infiltration, loosely stained cytoplasm, and glomerular basement membrane (GBM) thickening to near-normal levels.
Upregulated renal expression of circadian rhythm genes (Arntl, Cry1) and downregulated phosphorylated AKT/mTOR pathway components.
Molecular Weight

356.42

Formula

C20H24N2O4
CAS No.
Appearance

Solid

Color

Light yellow to light brown

SMILES

O=C(C1=C2[C@]3(C4=CC=CC(O)=C4N2)[C@@](C[C@@]1([H])[C@@]5([H])[C@H](O)C)([H])N(C5)CC3)OC
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Purity & Documentation
References
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19-epi-Scholaricine Related Classifications

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    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

19-epi-Scholaricine132923-06-9AktmTORApoptosisReactive Oxygen Species (ROS)CDKRasHSPVEGFRTNF ReceptorInterleukin RelatedPKBProtein kinase BMammalian target of RapamycinCyclin dependent kinaseHeat shock proteinsVascular endothelial growth factor receptorTumor Necrosis Factor ReceptorTNFRILHRASAKTARNTLKDRpodocytechronic glomerulonephritisHSP90AA1CDK2CRY1Inhibitorinhibitorinhibit

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