1. Metabolic Enzyme/Protease
  2. Cytochrome P450
  3. 5,7-Dimethoxyflavone

5,7-Dimethoxyflavone is one of the major components of Kaempferia parviflora, has anti-obesity, anti-inflammatory, and antineoplastic effects. 5,7-Dimethoxyflavone inhibits cytochrome P450 (CYP) 3As. 5,7-Dimethoxyflavone is also a potent Breast Cancer Resistance Protein (BCRP) inhibitor.

For research use only. We do not sell to patients.

5,7-Dimethoxyflavone Chemical Structure

5,7-Dimethoxyflavone Chemical Structure

CAS No. : 21392-57-4

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10 mM * 1 mL in DMSO
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Customer Review

Based on 1 publication(s) in Google Scholar

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Description

5,7-Dimethoxyflavone is one of the major components of Kaempferia parviflora, has anti-obesity, anti-inflammatory, and antineoplastic effects. 5,7-Dimethoxyflavone inhibits cytochrome P450 (CYP) 3As. 5,7-Dimethoxyflavone is also a potent Breast Cancer Resistance Protein (BCRP) inhibitor[1][2].

IC50 & Target[1][2]

CYP3A

 

Breast Cancer Resistance Protein (BCRP)

 

In Vitro

5,7-Dimethoxyflavone (5 and 50 μM, 1 h) inhibits Bcrp1-mediated Sorafenib efflux in MDCK/Bcrp1 cells[2].
5,7-Dimethoxyflavone (0-25 μM, 48 h ) induces melanogenesis by increasing cAMP in B16F10 melanoma cells[4].
5,7-Dimethoxyflavone (0-20 μM, 8 days) inhibits lipid accumulation by reducing TG accumulation in 3T3-L1 adipocytes, and inhibits adipocyte differentiation[5].
5,7-Dimethoxyflavone (0-100 μM, 48 h) inhibits proliferation, induces oxidative stress and induces apoptosis in VK2/E6E7 and End1/E6E7 cells[6].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis[4]

Cell Line: B16F10 melanoma cells
Concentration: 0-25 μM
Incubation Time: 48 h
Result: Induced CREB phosphorylation, and inhibited Akt and GSK-3β phosphorylation.
In Vivo

5,7-Dimethoxyflavone (10 mg/kg, p.o., once a day for 10 days) reduces liver CYP3A11 and CYP3A25 protein expression levels in mice[1].
5,7-Dimethoxyflavone (25 and 50 mg/kg, p.o.) inhibits sarcopenia in aged mice[3].
5,7-Dimethoxyflavone (50 mg/kg/day, p.o., for 6 weeks) decreases body weight gain and inhibits fatty liver in HFD mice[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Aged mice[3]
Dosage: 25 and 50 mg/kg
Administration: p.o.
Result: Increased grip strength and the running endurance.
Increased muscle volume, weights, and cross-sectional area.
Decreased serum and gastrocnemius muscle levels of IL-6 and TNF-α.
Molecular Weight

282.29

Formula

C17H14O4

CAS No.
Appearance

Solid

Color

White to yellow

SMILES

O=C1C=C(C2=CC=CC=C2)OC3=CC(OC)=CC(OC)=C13

Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 62.5 mg/mL (221.40 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.5425 mL 17.7123 mL 35.4246 mL
5 mM 0.7085 mL 3.5425 mL 7.0849 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (8.86 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 2.5 mg/mL (8.86 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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mg/kg

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(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.86%

References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.5425 mL 17.7123 mL 35.4246 mL 88.5614 mL
5 mM 0.7085 mL 3.5425 mL 7.0849 mL 17.7123 mL
10 mM 0.3542 mL 1.7712 mL 3.5425 mL 8.8561 mL
15 mM 0.2362 mL 1.1808 mL 2.3616 mL 5.9041 mL
20 mM 0.1771 mL 0.8856 mL 1.7712 mL 4.4281 mL
25 mM 0.1417 mL 0.7085 mL 1.4170 mL 3.5425 mL
30 mM 0.1181 mL 0.5904 mL 1.1808 mL 2.9520 mL
40 mM 0.0886 mL 0.4428 mL 0.8856 mL 2.2140 mL
50 mM 0.0708 mL 0.3542 mL 0.7085 mL 1.7712 mL
60 mM 0.0590 mL 0.2952 mL 0.5904 mL 1.4760 mL
80 mM 0.0443 mL 0.2214 mL 0.4428 mL 1.1070 mL
100 mM 0.0354 mL 0.1771 mL 0.3542 mL 0.8856 mL
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5,7-Dimethoxyflavone Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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5,7-Dimethoxyflavone
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