1. Membrane Transporter/Ion Channel
    Autophagy
  2. TRP Channel
    Autophagy

SKF-96365 hydrochloride 

Cat. No.: HY-100001 Purity: 99.73%
Handling Instructions

SKF-96365 hydrochloride, a potent TRP Channel blocker, exhibits potent anti-neoplastic activity by inducing cell-cycle arrest and apoptosis in colorectal cancer cells.

For research use only. We do not sell to patients.
SKF-96365 hydrochloride Chemical Structure

SKF-96365 hydrochloride Chemical Structure

CAS No. : 130495-35-1

Size Price Stock Quantity
Free Sample (0.5-1 mg)   Apply now  
10 mM * 1 mL in DMSO USD 79 In-stock
5 mg USD 72 In-stock
10 mg USD 120 In-stock
50 mg USD 468 In-stock
100 mg USD 780 In-stock
200 mg USD 1020 In-stock
500 mg   Get quote  
1 g   Get quote  

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Customer Review

  • Biological Activity

  • Protocol

  • Technical Information

  • Purity & Documentation

  • References

Description

SKF-96365 hydrochloride, a potent TRP Channel blocker, exhibits potent anti-neoplastic activity by inducing cell-cycle arrest and apoptosis in colorectal cancer cells.

IC50 & Target

TRP Channel[1]

In Vitro

SKF-96365 exhibits potent anti-neoplastic activity by inducing cell-cycle arrest and apoptosis in colorectal cancer cells. SKF-96365 inhibits hERG current in a concentration-dependent manner[1]. SKF-96365 can induces cytoprotective autophagy to delay apoptosis by preventing the release of cytochrome c (cyt c) from the mitochondria into the cytoplasm. Mechanistically, SKF-96365 treatment inhibits the calcium/calmodulin-dependent protein kinase IIγ (CaMKIIγ)/AKT signaling cascade. Overexpression of CaMKIIγ or AKT abolishes the effects of SKF-96365 on cancer cells, suggesting a critical role of the CaMKIIγ/AKT signaling pathway in SFK-96365-induced biological effects[2].

In Vivo

SKF-96365 inhibits CRC cell growth in vivo. SKF-96365 treatment results in a decrease of p-CaMKII and p-AKT as well as an increase in LC3-II, cleaved PARP, caspase-3, and caspase-9 in mice[2].

References
Preparing Stock Solutions
Concentration Volume Mass 1 mg 5 mg 10 mg
1 mM 2.4819 mL 12.4097 mL 24.8194 mL
5 mM 0.4964 mL 2.4819 mL 4.9639 mL
10 mM 0.2482 mL 1.2410 mL 2.4819 mL
Please refer to the solubility information to select the appropriate solvent.
Animal Administration
[2]

SKF-96365 is prepared in saline.

Mice: Five to six-week-old female athymic BALB/c mice are inoculated into the right oxter with HCT116 cells. When the diameter of the subcutaneous tumor reaches approximately 0.5 cm, animals are randomLy assigned to the vehicle, SKF-96365 alone, HCQ alone or SKF-96365+HCQ. SKF-96365 is applied (20 mg/kg) and HCQ is applied (60 mg/kg) daily for 14 successive days by i.p. injection. Tumor sizes and volume are determined. Eight mice are included in each group. Mice are sacrificed 24 h after the last treatment. The tumors are weighed and processed for western blot analysis or paraffin embedding[2]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
Molecular Weight

402.91

Formula

C₂₂H₂₇ClN₂O₃

CAS No.

130495-35-1

SMILES

COC1=CC=C(CCCOC(C2=CC=C(OC)C=C2)CN3C=CN=C3)C=C1.[H]Cl

Storage
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
Shipping

Room temperature in continental US; may vary elsewhere

Solvent & Solubility

DMSO: ≥ 30 mg/mL

* "<1 mg/mL" means slightly soluble or insoluble. "≥" means soluble, but saturation unknown.

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Product Name:
SKF-96365 hydrochloride
Cat. No.:
HY-100001
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