Acetazolamide sodium

Name: Acetazolamide sodium
Brand: MedChemExpress
SKU: HY-B0782A
Rating: 5.0 (13 reviews)

Cat. No.: HY-B0782A Purity: 99.54%: Data Sheet
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Acetazolamide sodium is a carbonic anhydrase (CA) IX inhibitor with an IC₅₀ of 30 nM for hCA IX. Acetazolamide sodium has diuretic, antihypertensive and anti-gonococcal activities.

For research use only. We do not sell to patients.

CAS No. : 1424-27-7

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
500 mg	In-stock	Estimated Time of Arrival: December 31
1 g	In-stock	Estimated Time of Arrival: December 31
5 g	In-stock	Estimated Time of Arrival: December 31
10 g	Get quote
50 g	Get quote

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Customer Review

Based on 13 publication(s) in Google Scholar

Other Forms of Acetazolamide sodium:

Acetazolamide-d₃ In-stock
Acetazolamide In-stock

13 Publications Citing Use of MCE Acetazolamide sodium

Bio/Physico-chemical Assay

: Acetazolamide sodium purchased from MedChemExpress. Usage Cited in: Antioxidants (Basel). 2024 Apr 17;13(4):473.; Effect of Acetazolamide, a CA inhibitor, on oxidation of PTEN by H₂O₂. HepG2 cells were pre-incubated in HCO₃⁻-free media, supplemented with 0, 0.01, 0.1, 1, and 10 µM Acetazolamide, at 37 °C with 0.1% CO₂ for 4 h. Then, they were changed to stimulation media with same components plus 0.5 mM H₂O₂. After 10 min, the reaction was terminated by NEM-containing lysis buffer. Western blot data with PTEN and β-actin antibodies are shown Acetazolamide considerably impeded PTEN oxidation in a dose-dependent manner.

: Acetazolamide sodium purchased from MedChemExpress. Usage Cited in: Antioxidants (Basel). 2024 Apr 17;13(4):473.; Effect of Acetazolamide, a CA inhibitor, on oxidation of PTEN by H₂O₂. HepG2 cells were pre-incubated in HCO₃⁻-free media, supplemented with 0, 0.01, 0.1, 1, and 10 µM Acetazolamide, at 37 °C with 0.1% CO₂ for 4 h. Then, they were changed to stimulation media with same components plus 0.5 mM H₂O₂. After 10 min, the reaction was terminated by NEM-containing lysis buffer. Western blot data with PTEN and β-actin antibodies are shown. Stars are considered as significances.

: Acetazolamide sodium purchased from MedChemExpress. Usage Cited in: Cell Rep. 2023 May 17;42(5):112529. [Abstract]; GSIS was measured via static incubation in male human islets (n = 3–7 donors, 10 IEQ/condition measured in triplicate) cultured for 24 h with vehicle or DHT (10 nM) and then for 40 min in the presence or absence of a carbonic anhydrase inhibitor Acetazolamide (50 μM).

: Acetazolamide sodium purchased from MedChemExpress. Usage Cited in: Elife. 2018 Feb 2;7:e33432. [Abstract]; Intracellular pH (pHi) of the ligated efferent ductules with or without Acetazolamide(500 μM) from WT mice and Adgrg2^-/Y mice.

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•Related Screening Libraries:

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View All Carbonic Anhydrase Isoform Specific Products:

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CA CA I CA Ⅱ CA IX CA XII CA VII CA VI CA VA CA VB CA XIII CA XIV

Biological Activity
Purity & Documentation
References
Customer Review

Description

Acetazolamide sodium is a carbonic anhydrase (CA) IX inhibitor with an IC₅₀ of 30 nM for hCA IX. Acetazolamide sodium has diuretic, antihypertensive and anti-gonococcal activities^[1]^[4]^[5]^[6].

IC₅₀ & Target

CA IX

In Vitro

Acetazolamide also inhibits hCA II with an IC₅₀ of 130 nM^[1].
? Acetazolamide (Ace) is a small heteroaromatic sulfonamide that binds to various carbonic anhydrases with high affinity, acting as a carbonic anhydrase (CA) inhibitor^[2].
? Compared with the control group, the high Acetazolamide concentration (AceH, 50 nM), Cisplatin (Cis; 1 μg/mL) and Cis combined with the low Acetazolamide concentration (AceL, 10 nM) treatments significantly reduces viability of Hep-2 cells^[2].
? Treatment with the Acetazolamide/Cis combination significantly increases the expression levels of P53, as both AceL+Cis and AceH+Cis treatments result in significantly increased P53 protein expression levels compared with the control group. The Ace/Cis combination treatment significantly reduces the bcl-2/bax expression ratio, and increases the expression of caspase-3 protein, compared with the control group. AceL, AceH, Cis and AceL+Cis treatments significantly reduce the bcl-2/bax ratio compared with the control group^[2].
? Combined Ace and Cis treatment effectively promotes apoptosis in Hep-2 cells^[2].
? Combined treatment with Ace/Cis markedly decreases the expression of AQP1 mRNA in Hep-2 cells. Both AceH and AceL+Cis treatments decrease the expression of aquaporin-1 (AQP1) mRNA in Hep-2 cells compared with the control group^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Acetazolamide (40 mg/kg) significantly potentiates the inhibitory effect of MS-275 on tumorigenesis in neuroblastoma (NB) SH-SY5Y xenografts^[3].
? Acetazolamide (40 mg/kg) and/or MS-275 treatment reduce expression of HIF1-α and CAIX in NB SH-SY5Y xenograft^[3].
? Acetazolamide (40 mg/kg), MS-275 and Acetazolamide+MS-275 reduce expression of mitotic and proliferative markers in NB SH-SY5Y xenografts^[3].? Acetazolamide (50 mg/kg; PO, for 3 days) significantly reduces the gonococcal load in the vagina of infected mice by 90%^[6].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

245.24

Formula

C₄H₆N₄NaO₃S₂

CAS No.

1424-27-7

Appearance

Solid

Color

White to off-white

SMILES

CC(NC1=NN=C(S(=O)(N)=O)S1)=O.[Na]

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

H₂O : ≥ 100 mg/mL (407.76 mM)

DMSO : 100 mg/mL (407.76 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	4.0776 mL	20.3882 mL	40.7764 mL
5 mM	0.8155 mL	4.0776 mL	8.1553 mL
10 mM	0.4078 mL	2.0388 mL	4.0776 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.54%

Select Batch:

Data Sheet (278 KB) SDS (252 KB)

COA (250 KB) HNMR (254 KB) LCMS (95 KB)

Handling Instructions (2659 KB)

References

[1]. Bayat Mokhtari R, et al. Acetazolamide potentiates the anti-tumor potential of HDACi, MS-275, in neuroblastoma. BMC Cancer. 2017 Feb 24;17(1):156. [Content Brief]

[2]. Hou Z, et al. Dual-tail approach to discovery of novel carbonic anhydrase IX inhibitors by simultaneously matching the hydrophobic and hydrophilic halves of the active site. Eur J Med Chem. 2017 May 26;132:1-10. [Content Brief]

[3]. Gao H, et al. Combined treatment with acetazolamide and cisplatin enhances chemosensitivity in laryngeal carcinoma Hep-2 cells. Oncol Lett. 2018 Jun;15(6):9299-9306. [Content Brief]

[4]. Kassamali R, et al. Acetazolamide: a forgotten diuretic agent. Cardiol Rev. 2011 Nov-Dec;19(6):276-8. [Content Brief]

[5]. Jabeen E, et al. Interaction of antihypertensive acetazolamide with nonsteroidal anti-inflammatory drugs. J Photochem Photobiol B. 2013 Aug 5;125:155-63. [Content Brief]

[6]. Abutaleb NS, et al. In vivo efficacy of acetazolamide in a mouse model of Neisseria gonorrhoeae infection. Microb Pathog. 2022 Mar;164:105454. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	4.0776 mL	20.3882 mL	40.7764 mL	101.9410 mL
	5 mM	0.8155 mL	4.0776 mL	8.1553 mL	20.3882 mL
	10 mM	0.4078 mL	2.0388 mL	4.0776 mL	10.1941 mL
	15 mM	0.2718 mL	1.3592 mL	2.7184 mL	6.7961 mL
	20 mM	0.2039 mL	1.0194 mL	2.0388 mL	5.0970 mL
	25 mM	0.1631 mL	0.8155 mL	1.6311 mL	4.0776 mL
	30 mM	0.1359 mL	0.6796 mL	1.3592 mL	3.3980 mL
	40 mM	0.1019 mL	0.5097 mL	1.0194 mL	2.5485 mL
	50 mM	0.0816 mL	0.4078 mL	0.8155 mL	2.0388 mL
	60 mM	0.0680 mL	0.3398 mL	0.6796 mL	1.6990 mL
	80 mM	0.0510 mL	0.2549 mL	0.5097 mL	1.2743 mL
	100 mM	0.0408 mL	0.2039 mL	0.4078 mL	1.0194 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.