2. MAPK/ERK Pathway PI3K/Akt/mTOR Apoptosis Autophagy NF-κB Metabolic Enzyme/Protease Immunology/Inflammation
  3. JNK Akt Apoptosis Autophagy Reactive Oxygen Species
  4. Actein

Actein, a triterpene glycoside, shows an inhibitory effect on cancer cells, which is isolated from the rhizomes of Cimicifuga foetida. Actein suppresses cell proliferation, induces autophagy and apoptosis through promoting ROS/JNK activation, and blunting AKT pathway in bladder cancer. Actein has little toxicity in vivo.

For research use only. We do not sell to patients.

Actein Chemical Structure

Actein Chemical Structure

CAS No. : 18642-44-9

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Based on 1 publication(s) in Google Scholar

Actein Related Antibodies

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Description

Actein, a triterpene glycoside, shows an inhibitory effect on cancer cells, which is isolated from the rhizomes of Cimicifuga foetida. Actein suppresses cell proliferation, induces autophagy and apoptosis through promoting ROS/JNK activation, and blunting AKT pathway in bladder cancer. Actein has little toxicity in vivo[1][2][3].

In Vitro

Actein (0-30 µM, 24 h) significantly suppress cell proliferation and induce cell apoptosis of gastric cancer cells via Caspase-3 and p53 signaling pathway combined with tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)[1].
Actein (0-40 μM, 24 h) suppresses cell proliferation, induces G2/M cell cycle arrest, autophagy and apoptosis in bladder cancer cells[2].
Actein (0-20 μM, 24 h) potentiates ROS generation and JNK phosphorylation and suppresses AKT pathway in BIU-87 and T24 cells[2].
Actein (10 nM and 10 μM, 5-10 min) increases calcium release into the cytoplasm of breast cancer cells and colon cancer cells[3].
Actein (0-40 μg/mL, 48 h) inhibits the NF-κB and MEK pathways by decreasing the level of IKKβ protein in 293T (null) and 293T (NF-κB) cells[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Actein Related Antibodies

Cell Viability Assay[1]

Cell Line: SNU-216 and AGS gastric cancer cell lines
Concentration: 0-30 µM
Incubation Time: 24 h
Result: Exhibited a strong inhibitory effect on gastric cancer cell proliferation in a dose-dependent manner combined with TRAIL in gastric cancer cells.

Immunofluorescence[2]

Cell Line: BIU-87 and T24 cells
Concentration: 0-20 μM
Incubation Time: 24 h
Result: Exhibited dose-increased levels of oxidation-sensitive red fluorescence, indicating the elevation of ROS in BIU-87 and T24 cells.

Western Blot Analysis[1]

Cell Line: SNU-216 and AGS gastric cancer cell lines
Concentration: 0-30 µM
Incubation Time: 24 h
Result: Dose-dependently reduced the AKT/mTOR and JAK2/STAT3 activity, while p38 and JNK were highly phosphorylated in BIU-87 and T24 cells.

Western Blot Analysis[3]

Cell Line: 293T (null) and 293T (NF-κB) cells
Concentration: 0-40 μg/mL
Incubation Time: 48 h
Result: Decreased the level of IKKβ protein in a dose dependent manner in 293T (null) and 293T (NF-κB) cells.
Molecular Weight

676.83

Formula

C37H56O11
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

C[C@]12[C@@]3([H])[C@@]4(C[C@@H](OC(C)=O)[C@@]1([C@]5([H])[C@](O[C@]6([C@H]7[C@]([C@@H](O)O6)(C)O7)C[C@H]5C)([H])C2)C)[C@@]8([C@@](C(C)([C@@H](O[C@@]9([H])[C@@H]([C@H]([C@H](O)CO9)O)O)CC8)C)([H])CC3)C4
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (147.75 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.4775 mL 7.3874 mL 14.7748 mL
5 mM 0.2955 mL 1.4775 mL 2.9550 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Purity & Documentation

Purity: 99.25%

COA (254 KB) RP-HPLC (213 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.4775 mL 7.3874 mL 14.7748 mL 36.9369 mL
5 mM 0.2955 mL 1.4775 mL 2.9550 mL 7.3874 mL
10 mM 0.1477 mL 0.7387 mL 1.4775 mL 3.6937 mL
15 mM 0.0985 mL 0.4925 mL 0.9850 mL 2.4625 mL
20 mM 0.0739 mL 0.3694 mL 0.7387 mL 1.8468 mL
25 mM 0.0591 mL 0.2955 mL 0.5910 mL 1.4775 mL
30 mM 0.0492 mL 0.2462 mL 0.4925 mL 1.2312 mL
40 mM 0.0369 mL 0.1847 mL 0.3694 mL 0.9234 mL
50 mM 0.0295 mL 0.1477 mL 0.2955 mL 0.7387 mL
60 mM 0.0246 mL 0.1231 mL 0.2462 mL 0.6156 mL
80 mM 0.0185 mL 0.0923 mL 0.1847 mL 0.4617 mL
100 mM 0.0148 mL 0.0739 mL 0.1477 mL 0.3694 mL
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Actein Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Actein18642-44-9JNKAktApoptosisAutophagyReactive Oxygen Speciesc-Jun N-terminal kinasePKBProtein kinase Btriterpene glycosidecancer cellscell proliferationautophagyapoptosisROS/JNK activationAKT pathwayInhibitorinhibitorinhibit

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