Antiproliferative agent-84
Antiproliferative agent-84 is a 3‑cyanopyridine-based multi-target kinase inhibitor that acts on EGFR (IC50 = 0.300 μM), AKT (IC50 = 0.706 μM), ERK (IC50 = 0.143 μM), and p38‑MAPKα (IC50 = 0.308 μM). Antiproliferative agent-84 exhibits selective antiproliferative activity against colorectal cancer and breast cancer cells, inducing apoptosis and cell cycle arrest. Antiproliferative agent-84 can be used for research on colorectal cancer and breast cancer.
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- Formule: C33H26N2O2
- Masse moléculaire:482.57
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Stockage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Activité biologique
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Chemical Information
- Protocole
- Pureté et documentation
- Références
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Activité biologique
Description
IC50 & Target
[1]|
EGFR 0.300 μM (IC50) |
ERK 0.143 μM (IC50) |
Akt 0.706 μM (IC50) |
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| HCT-116 | IC50 |
7.34 μM
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Cytotoxicity against human HCT116 colorectal cancer cells assessed as viability reduction incubated 48 hrs by MTT assay.
Cytotoxicity against human HCT116 colorectal cancer cells assessed as viability reduction incubated 48 hrs by MTT assay.
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42743607 |
| MCF7 | IC50 |
17.55 μM
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Cytotoxicity against human MCF-7 breast cancer cells assessed as viability reduction incubated 48 hrs by MTT assay.
Cytotoxicity against human MCF-7 breast cancer cells assessed as viability reduction incubated 48 hrs by MTT assay.
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42743607 |
| WI-38 | IC50 |
94.83 μM
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Cytotoxicity against normal human WI38 lung fibroblasts assessed as viability reduction incubated 48 hrs by MTT assay.
Cytotoxicity against normal human WI38 lung fibroblasts assessed as viability reduction incubated 48 hrs by MTT assay.
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42743607 |
In Vitro
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:HCT116 colorectal cancer cells
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Concentration:7.34 μM (IC50)
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Incubation Time:24 h
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Result:Increased the G0/G1 population from 54.62% in untreated HCT116 cells to 77.59%.
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Cell Line:HCT116 colorectal cancer cells
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Concentration:7.34 μM (IC50)
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Incubation Time:24 h
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Result:Incubation of HCT116 cells with compound 2b produced 15.35% early apoptotic cells and 5.24% late apoptotic cells.
- Detection of Apoptosis
- TUNEL staining for apoptotic DNA fragmentation
- Flow cytometric DNA-content cell-cycle staining
- Annexin V plus membrane-impermeant dye apoptosis staining
- BrdU Incorporation Assay
- Apoptosis Solutions
- Protocol for Cell Cycle
- Breast Cancer Modeling
- Cell Viability Determination by MTT Colorimetric Assay
Chemical Information
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Masse moléculaire 482.57
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Formule C33H26N2O2
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SMILES
CC(C=C1)=CC=C1C2=NC(OCC3=CC=CC=C3)=C(C#N)C(C(C=C4)=CC=C4OCC5=CC=CC=C5)=C2
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Please store the product under the recommended conditions in the Certificate of Analysis.
Pureté et documentation
Références
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Antiproliferative agent-84 Related Classifications
HY-189638 Related Classifications
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