Art. -Nr.: HY-15398

Vitamin D3

(Synonyms: Cholecalciferol; Colecalciferol)

23 Cited Publications

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Based on 23 publication(s) in Google Scholar

Vitamin D3 (Cholecalciferol; Colecalciferol) is a naturally occuring form of vitamin D. Vitamin D3 induces cell differentiation and prevents proliferation of cancer cells.

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Reinheit: 99.94%
CAS. Nr.: 67-97-0
Formel: C₂₇H₄₄O
Molecular Weight:384.64

Speicherung:

4°C, protect from light, stored under nitrogen

* The compound is unstable in solutions, freshly prepared is recommended.

Vitamin D3 Chemical Structure

CAS. Nr.: 67-97-0

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Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
- Clinical Trial
Chemical Information
Publications (23)
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

Vitamin D3 (Cholecalciferol; Colecalciferol) is a naturally occuring form of vitamin D. Vitamin D3 induces cell differentiation and prevents proliferation of cancer cells.

IC₅₀ & Target

Human Endogenous Metabolite

Alle Endogenous Metabolite Isoform-spezifische Produkte anzeigen :

Cellular Effect

Cell Line	Type	Value	Description	References
C3H 10T1/2	IC₅₀	2.9 μM Compound: 3, VD3	Inhibition of Hedgehog signaling in mouse C3H10T1/2 cells assessed as downregulation of Ptch mRNA expression after 24 hrs by RT-PCR analysis Inhibition of Hedgehog signaling in mouse C3H10T1/2 cells assessed as downregulation of Ptch mRNA expression after 24 hrs by RT-PCR analysis	[PMID: 24900716]
C3H 10T1/2	IC₅₀	2.9 μM Compound: 3, VD3, Vitamin D3	Inhibition of hedgehog signaling pathway-mediated PTCH mRNA expression in mouse C3H10T1/2 cells after 24 hrs by RT-PCR analysis Inhibition of hedgehog signaling pathway-mediated PTCH mRNA expression in mouse C3H10T1/2 cells after 24 hrs by RT-PCR analysis	[PMID: 24730984]
C3H 10T1/2	IC₅₀	4.1 μM Compound: 2, VD3, vitamin D3	Inhibition of hedgehog signaling in mouse C3H10T1/2 cells assessed as reduction in Gli1 mRNA level after 24 hrs by quantitative PCR analysis Inhibition of hedgehog signaling in mouse C3H10T1/2 cells assessed as reduction in Gli1 mRNA level after 24 hrs by quantitative PCR analysis	[PMID: 25676864]
C3H 10T1/2	IC₅₀	4.1 μM Compound: 3, VD3	Inhibition of Hedgehog signaling in mouse C3H10T1/2 cells assessed as downregulation of Gli1 mRNA expression after 24 hrs by RT-PCR analysis Inhibition of Hedgehog signaling in mouse C3H10T1/2 cells assessed as downregulation of Gli1 mRNA expression after 24 hrs by RT-PCR analysis	[PMID: 24900716]
C3H 10T1/2	IC₅₀	4.1 μM Compound: 3, VD3, Vitamin D3	Inhibition of hedgehog signaling pathway-mediated GLI1 mRNA expression in mouse C3H10T1/2 cells after 24 hrs by RT-PCR analysis Inhibition of hedgehog signaling pathway-mediated GLI1 mRNA expression in mouse C3H10T1/2 cells after 24 hrs by RT-PCR analysis	[PMID: 24730984]
Daoy	IC₅₀	>10 μM Compound: 3, VD3	Inhibition of Hedgehog signaling in human DaOY cells assessed as downregulation of Gli1 mRNA expression after 48 hrs by RT-PCR analysis Inhibition of Hedgehog signaling in human DaOY cells assessed as downregulation of Gli1 mRNA expression after 48 hrs by RT-PCR analysis	[PMID: 24900716]
Daoy	IC₅₀	>10 μM Compound: 3, VD3, Vitamin D3	Inhibition of hedgehog signaling pathway-mediated GLI1 mRNA expression in human DaOY cells after 24 hrs by RT-PCR analysis Inhibition of hedgehog signaling pathway-mediated GLI1 mRNA expression in human DaOY cells after 24 hrs by RT-PCR analysis	[PMID: 24730984]
Daoy	IC₅₀	>10 μM Compound: 3, VD3, Vitamin D3	Inhibition of hedgehog signaling pathway-mediated PTCH mRNA expression in human DaOY cells after 24 hrs by RT-PCR analysis Inhibition of hedgehog signaling pathway-mediated PTCH mRNA expression in human DaOY cells after 24 hrs by RT-PCR analysis	[PMID: 24730984]
HEK293	EC₅₀	0.21 nM Compound: VitD3	Agonist activity at human VDR expressed in HEK293 cells by luciferase reporter gene assay Agonist activity at human VDR expressed in HEK293 cells by luciferase reporter gene assay	[PMID: 25305688]
HT-29	GI₅₀	53.8 μM Compound: 2, VD3	Growth inhibition of human HT-29 cells after 72 hrs by MTS-PMS assay Growth inhibition of human HT-29 cells after 72 hrs by MTS-PMS assay	[PMID: 23972439]
HT-29	GI₅₀	68.5 μM Compound: VD3	Growth inhibition of human HT-29 cells overexpressing VDR gene after 72 hrs by MTS assay Growth inhibition of human HT-29 cells overexpressing VDR gene after 72 hrs by MTS assay	[PMID: 22687748]
Medulloblastoma cell	GI₅₀	5.8 μM Compound: 1; VD3	Antiproliferative activity against medulloblastoma cells derived from conditional patched knockout mouse after 48 hrs by [3H]-thymidine incorporation assay Antiproliferative activity against medulloblastoma cells derived from conditional patched knockout mouse after 48 hrs by [3H]-thymidine incorporation assay	[PMID: 30471551]
U-87MG ATCC	GI₅₀	23.2 μM Compound: VD3	Growth inhibition of human U87MG cells after 72 hrs by MTS assay Growth inhibition of human U87MG cells after 72 hrs by MTS assay	[PMID: 22687748]
U-87MG ATCC	IC₅₀	29.7 μM Compound: VD3	Antiproliferative activity against human U87MG cells after 72 hrs by MTS assay Antiproliferative activity against human U87MG cells after 72 hrs by MTS assay	[PMID: 22226657]

In Vitro

Vitamin D3 is an inactive vitamin D molecule in vivo. Vitamin D3 undergoes two hydroxylation processes to activate it. Vitamin D3 is first hydroxylated in the liver to form the circulating prohormone 25-hydroxy vitamin D3 [25(OH)D3] by the enzyme 25-hydroxylase (CYP27A1) and probably also by other enzymes (e.g., CYP2R1)^[1].
The second hydroxylation occurs in the kidneys via the enzyme 1-alpha-hydroxylase, yielding 1,25- dihydroxycholecalciferol (calcitriol), which is the biologically active form of vitamin D^[1].
Vitamin D3 (2-10 μM; 24-48 hours) exhibits anti-proliferative effects in a dose- and time-dependent manner. Maximal reduction of viability post-treatment of 62% (IK), 52% (RL-95-2), and 55% (Hec-1A) occurs by 72 h of treatment with 10 μM Vitamin D3. but 24-hour exposure lacks significant reduction in viable cells^[2].
Cholecalciferol (10 μM; 24-48 hours) shows marked increases in nuclear VDR staining and produces local VDR activation in IK cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[2]

Cell Line:

EC cell lines from EEC, Ishikawa 3-H-12(IK), RL-95/2, and HEC-1-A cells
Concentration:

2-10 μM
Incubation Time:

24-72 hours
Result:

Reduced viability in response to VD3 in a dose- and time-dependent manner.
Indicated that the conversion of VD3 to 25(OH)D is an essential step for the reduced cell viability effect.

Cell Viability Assay^[2]

Cell Line:

EC cell lines from EEC, Ishikawa 3-H-12(IK) cells
Concentration:

10 μM
Incubation Time:

24-48 hours
Result:

Improved nuclear VDR content in IK cells.

In Vivo

Cholecalciferol (oral gavage; 5 mg/kg; 7 days) potentiates the CCl4 toxicity only in the liver, as indicated by plasma levels of ALT and AST, biochemical markers of hepatic damage. It significantly increases renal calcium levels in mice, but renal calcium content does not differ significantly between mice^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Male ddY mice on CCl4 toxicity^[3]
Dosage:

5 mg/kg
Administration:

Oral gavage; 5 mg/kg; 7 days
Result:

Potentiated CCl4-induced hepatotoxicity and enhanced mouse mortality, without increasing renal toxicity and generation of liver fibrosis.

Clinical Trial

NCT Number	Sponsor	Condition	Start Date	Phase A549
NCT01329991	Plexxikon\|		2011-05	PHASE1

Chemical Information

CAS. Nr. 67-97-0
Appearance Solid
Molecular Weight 384.64
Formel C₂₇H₄₄O
Color White to off-white
SMILES

C[C@@]12[C@](CC[C@]2([H])[C@H](C)CCCC(C)C)([H])/C(CCC1)=C/C=C3C(CC[C@@H](C\3)O)=C
Synonyms

Cholecalciferol; Colecalciferol
Structure Classification
- Others
Initial Source
- Endogenous metabolite
- Human Gut Microbiota Metabolites
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung

4°C, protect from light, stored under nitrogen

* The compound is unstable in solutions, freshly prepared is recommended.

Publications (23)

All In Vivo Efficacy StudyIHCRT-PCR

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Journal Impact Factor

Cell Metab

Sensing steroid hormone 17α-hydroxypregnenolone by GPR56 enables protection from ferroptosis-induced liver injury. [Abstract]

2024 Nov 5;36(11):2402-2418.e10. PMID: 39389061
Nat Chem Biol

Structures of the ADGRG2-Gs complex in apo and ligand-bound forms. [Abstract]

2022 Nov;18(11):1196-1203. PMID: 35982227
Theranostics

Vitamin D binding protein (VDBP) hijacks twist1 to inhibit vasculogenic mimicry in hepatocellular carcinoma. [Abstract]

2024 Jan 1;14(1):436-450. PMID: 38164156

Vitamin D3 purchased from MedChemExpress. Usage Cited in: Theranostics. 2024 Jan 1;14(1):436-450. [Abstract]

Representative in vivo images of MHCC-97H-luc-tumor-bearing-BALB/c-nude mice with Vitamin D3 treatment (1500 IU/kg dissolved in absolute ethanol to the daily drinking water; continuously for 2 months) and quantified valuesare shown as relative luciferase activity.

Vitamin D3 purchased from MedChemExpress. Usage Cited in: Theranostics. 2024 Jan 1;14(1):436-450. [Abstract]

Representative images of CD31-PAS double staining, HE staining, IHC staining of VDBP, VE-cadherin, E-cadherin, MMP2 and MMP9 and PLA assays in liver tissues of MHCC-97H-luc-tumor-bearing-BALB/c-nude mice with Vitamin D3 treatment (1500 IU/kg dissolved in absolute ethanol to the daily drinking water; continuously for 2 months) .
Int J Oral Sci

Targeted inhibition of osteoclastogenesis reveals the pathogenesis and therapeutics of bone loss under sympathetic neurostress. [Abstract]

2022 Aug 1;14(1):39. PMID: 35915088
Proc Natl Acad Sci U S A

Progesterone activates GPR126 to promote breast cancer development via the Gi pathway. [Abstract]

2022 Apr 12;119(15):e2117004119. PMID: 35394864
Small Methods

Sympathetic Neurostress Drives Osteoblastic Exosomal MiR-21 Transfer to Disrupt Bone Homeostasis and Promote Osteopenia. [Abstract]

2022 Mar;6(3):e2100763. PMID: 35312228
Cell Death Discov

Niacin inhibits vascular calcification via modulating of SIRT1/SIRT6 signaling pathway. [Abstract]

2025 Dec 6. PMID: 41353172
Mol Med

Elabela alleviates cuproptosis and vascular calcification in vitaminD3- overloaded mice via regulation of the PPAR-γ /FDX1 signaling. [Abstract]

2024 Nov 20;30(1):223. PMID: 39567863
Cell Mol Life Sci

Dapagliflozin targets SGLT2/SIRT1 signaling to attenuate the osteogenic transdifferentiation of vascular smooth muscle cells. [Abstract]

2024 Nov 9;81(1):448. PMID: 39520538

Vitamin D3 purchased from MedChemExpress. Usage Cited in: Cell Mol Life Sci. 2024 Nov 9;81(1):448. [Abstract]

Levels of calcium content and ALP activity in aortic tissues from Vitamin D3 (VitD3; 5×10⁵ IU/kg; subcutaneous injection; three consecutive days)-overloaded mice.

Vitamin D3 purchased from MedChemExpress. Usage Cited in: Cell Mol Life Sci. 2024 Nov 9;81(1):448. [Abstract]

SIRT1, HIF-1α, RUNX2, BMP2, α-SMA, and SM22α protein levels in the aortic tissues from Vitamin D3 (VitD3; 5×10⁵ IU/kg; subcutaneous injection; three consecutive days)-overloaded mice were analyzed via Western immunoblotting.

Vitamin D3 purchased from MedChemExpress. Usage Cited in: Cell Mol Life Sci. 2024 Nov 9;81(1):448. [Abstract]

RUNX2, BMP2, α-SMA, and SM22α mRNA levels were analyzed in aortic tissues from Vitamin D3 (VitD3; 5×10⁵ IU/kg; subcutaneous injection; three consecutive days)-overloaded mice.
Int Immunopharmacol

PF4/CXCR3 signaling contributes to the regulation of endothelial cells on smooth muscle cells in CKD vascular calcification. [Abstract]

2026 Mar 1:172:116235. PMID: 41558292
Mol Nutr Food Res

Single Treatment of Vitamin D3 Ameliorates LPS-Induced Acute Lung Injury through Changing Lung Rodentibacter abundance. [Abstract]

2022 Feb;66(3):e2100952. PMID: 34894076
J Funct Foods

2025 Mar.
Virol Sin

Antiviral activity of vitamin D derivatives against severe fever with thrombocytopenia syndrome virus in vitro and in vivo. [Abstract]

2024 Aug 19:S1995-820X(24)00134-2. PMID: 39168248
ACS Chem Neurosci

Vitamin D Ameliorates Doxorubicin-Induced Cognitive Dysfunction via Modulation of the SFRP1/β-Catenin Axis. [Abstract]

2025 Oct 26. PMID: 41139396
Sci Rep

Knockdown of RGMa reduces vascular calcification by inhibiting the AKT signaling pathway. [Abstract]

2025 Jul 9;15(1):24657. PMID: 40634394
Fish Shellfish Immunol

Vitamin D3 can effectively and rapidly clear largemouth bass ranavirus by immunoregulation. [Abstract]

2023 Dec:143:109213. PMID: 37949380
ACS Chem Biol

2026 Apr 3;21(4):764-778.
Clin Rheumatol

1,25(OH)2D3 induces chondrocyte autophagy and reduces the loss of proteoglycans in osteoarthritis through inhibiting the NF-κB pathway. [Abstract]

2025 Feb;44(2):811-822. PMID: 39775461
Theriogenology

Effect of VD3 on cell proliferation and the Wnt signaling pathway in bovine endometrial epithelial cells treated with lipopolysaccharide. [Abstract]

2022 Nov:193:68-76. PMID: 36156426
Vet Immunol Immunopathol

Vitamin D3 mediates autophagy to alleviate inflammatory responses in bovine endometrial epithelial cells and organoids via the PI3K/AKT/mTOR pathway. [Abstract]

2024 Nov:277:110839. PMID: 39418864
bioRxiv

Closing the loop: Teaching single-cell foundation models to learn from perturbations. [Abstract]

2025 Jul 12:2025.07.08.663754. PMID: 40672312
Discover Electrochemistry

2025 Jun6.
bioRxiv

2025 January 10.

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 10 mg/mL (26.00 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.5998 mL

12.9992 mL

25.9983 mL

5 mM

0.5200 mL

2.5998 mL

5.1997 mL

10 mM

0.2600 mL

1.2999 mL

2.5998 mL

Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: 2.08 mg/mL (5.41 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.08 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 2

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 1 mg/mL (2.60 mM); Clear solution

This protocol yields a clear solution of ≥ 1 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (10.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 1 mg/mL (2.60 mM); Clear solution

This protocol yields a clear solution of ≥ 1 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (10.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (277 KB)
SDS (587 KB)
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COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Nazik Al-Hashimi, et al. Cholecalciferol

[2]. Laura Bergadà, et al. Role of local bioactivation of vitamin D by CYP27A1 and CYP2R1 in the control of cell growth in normal endometrium and endometrial carcinoma. Lab Invest. 2014 Jun;94(6):608-22 [Content Brief]

[3]. Hiroki Yoshioka, et al. Vitamin D3-induced hypercalcemia increases carbon tetrachloride-induced hepatotoxicity through elevated oxidative stress in mice. PLoS One. 2017 Apr 27;12(4):e0176524. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.5998 mL	12.9992 mL	25.9983 mL	64.9958 mL
	5 mM	0.5200 mL	2.5998 mL	5.1997 mL	12.9992 mL
	10 mM	0.2600 mL	1.2999 mL	2.5998 mL	6.4996 mL
	15 mM	0.1733 mL	0.8666 mL	1.7332 mL	4.3331 mL
	20 mM	0.1300 mL	0.6500 mL	1.2999 mL	3.2498 mL
	25 mM	0.1040 mL	0.5200 mL	1.0399 mL	2.5998 mL

Vitamin D3

23 Cited Publications

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Vitamin D3

MCE Bioactive Compound Library

Natural Product Library Plus

Drug Repurposing Compound Library Plus

FDA-Approved Drug Library Plus

Cosmetic Ingredient Compound Library

Publications Citing Use of MedChemExpress (MCE) Vitamin D3

Alle Endogenous Metabolite Isoform-spezifische Produkte anzeigen

Biologische Aktivität

Chemical Information

Publications (23)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

Vitamin D3

MCE Bioactive Compound Library

Natural Product Library Plus

Drug Repurposing Compound Library Plus

FDA-Approved Drug Library Plus

Cosmetic Ingredient Compound Library

Publications Citing Use of MedChemExpress (MCE) Vitamin D3

Alle Endogenous Metabolite Isoform-spezifische Produkte anzeigen

Biologische Aktivität

Chemical Information

Publications (23)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

Vitamin D3 Related Classifications

Art. -Nr.: HY-15398