2. Cell Cycle/DNA Damage Epigenetics Others
  3. HDAC Fluorescent Dye
  4. Boc-Lys(Ac)-AMC

Boc-Lys(Ac)-AMC is a cell-permeable fluorometric HDAC substrate (Ex/Em = 355 nm/460 nm).

For research use only. We do not sell to patients.

Boc-Lys(Ac)-AMC Chemical Structure

Boc-Lys(Ac)-AMC Chemical Structure

CAS No. : 233691-67-3

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Based on 1 publication(s) in Google Scholar

Boc-Lys(Ac)-AMC Related Antibodies

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HDAC HDAC1 HDAC2 HDAC3 HDAC4 HDAC5 HDAC6 HDAC7 HDAC8 HDAC9 HDAC10 HDAC11 HD1 HD2

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Boc-Lys(Ac)-AMC is a cell-permeable fluorometric HDAC substrate (Ex/Em = 355 nm/460 nm)[1][2].

In Vitro

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
Tissue culture-based HDAC assay[2]
1. Apply 25 μM Boc-Lys(Ac)-AMC to the overnight culture seeded from 6 × 104 cells in 100 mL medium in 96-well tissue culture plates.
2. Incubate 2-3 h at 37°C with 5% CO2 atmosphere.
3. Terminate the deacetylation reaction by addition of HDAC developer solution containing cell lysis buffer and trypsin reagents.
4. Incubate 15 min at room temperature.
5. Measure the fluorescent signal of AMC by SpectraMax M5 microplate reader at Ex/Em = 355 nm/460 nm.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Boc-Lys(Ac)-AMC Related Antibodies
Molecular Weight

445.51

Formula

C23H31N3O6
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

CC(C(C(O1)=C2)=CC=C2NC([C@H](CCCCNC(C)=O)NC(OC(C)(C)C)=O)=O)=CC1=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (224.46 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.2446 mL 11.2231 mL 22.4462 mL
5 mM 0.4489 mL 2.2446 mL 4.4892 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

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(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.2446 mL 11.2231 mL 22.4462 mL 56.1155 mL
5 mM 0.4489 mL 2.2446 mL 4.4892 mL 11.2231 mL
10 mM 0.2245 mL 1.1223 mL 2.2446 mL 5.6115 mL
15 mM 0.1496 mL 0.7482 mL 1.4964 mL 3.7410 mL
20 mM 0.1122 mL 0.5612 mL 1.1223 mL 2.8058 mL
25 mM 0.0898 mL 0.4489 mL 0.8978 mL 2.2446 mL
30 mM 0.0748 mL 0.3741 mL 0.7482 mL 1.8705 mL
40 mM 0.0561 mL 0.2806 mL 0.5612 mL 1.4029 mL
50 mM 0.0449 mL 0.2245 mL 0.4489 mL 1.1223 mL
60 mM 0.0374 mL 0.1871 mL 0.3741 mL 0.9353 mL
80 mM 0.0281 mL 0.1403 mL 0.2806 mL 0.7014 mL
100 mM 0.0224 mL 0.1122 mL 0.2245 mL 0.5612 mL
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Boc-Lys(Ac)-AMC Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Boc-Lys(Ac)-AMC233691-67-3HDACFluorescent DyeHistone deacetylasesInhibitorinhibitorinhibit

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