Anticancer agent 325
Anticancer agent 325 is an apoptosis/autophagy inducer and anticancer agent. Anticancer agent 325 exhibits cytotoxic activity against gastric cancer cells, with no significant effect on healthy gastric epithelial cells. Anticancer agent 325 enhances the expression of activated/cleaved Caspase-3 to promote apoptosis. Anticancer agent 325 also enhances the cleavage of PARP, a downstream marker of apoptosis, promotes LC3B lipidation, and activates autophagy. Anticancer agent 325 can be used in the research of gastric cancer.
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- CAS No.: 2922798-57-8
- Formule: C18H26O5
- Masse moléculaire:322.40
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Stockage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Activité biologique
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Chemical Information
- Protocole
- Pureté et documentation
- Références
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Activité biologique
Description
IC50 & Target
[1]|
Caspase 3 |
In Vitro
Anticancer agent 325 (compound 2d) (1.9-120.0 μM; 72 h) potently induces cytotoxicity in AGS and KATO III gastric cancer cells, with IC50 values of 30.9 μM and 19.0 μM, respectively; by contrast, it shows a higher IC50 value of 38.3 μM in non-malignant GES-1 gastric epithelial cells[1].
Anticancer agent 325 (30 μM; 24 h) modulates apoptosis and autophagy markers in AGS and KATO III gastric cancer cells, but exerts no such effect on non-cancerous GES-1 gastric epithelial cells[1].
Anticancer agent 325 (30 μM; 24 h) induces morphological changes of apoptosis and autophagosome formation in AGS gastric cancer cells, while it induces necrotic/apoptotic changes and autophagosome formation in KATO III gastric cancer cells, and also causes mitochondrial damage[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:human gastric adenocarcinoma AGS cells, human signet ring cell carcinoma KATO III cells, noncancerous gastric epithelial immortalized GES-1 cells
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Concentration:1.9, 3.8, 7.5, 15.0, 30.0, 60.0, 120.0 μM
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Incubation Time:72 h
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Result:Potently reduced viability of AGS cells with an IC50 of 30.9 μM.
Potently reduced viability of KATO III cells with an IC50 of 19.0 μM.
Reduced viability of GES-1 cells with an IC50 of 38.3 μM.
Induced dose-dependent cytotoxicity, with significant reductions in cell viability at multiple concentrations relative to untreated controls.
- RNA extraction experimental
- Detection of Apoptosis
- TUNEL staining for apoptotic DNA fragmentation
- Cell Cytotoxicity Assay
- Annexin V plus membrane-impermeant dye apoptosis staining
- Autophagy
- Apoptosis Solutions
- Lysosome and acidic-vesicle live-cell staining
- Macroautophagy Solutions
- Cell Viability Determination by MTT Colorimetric Assay
Chemical Information
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CAS No. 2922798-57-8
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Masse moléculaire 322.40
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Formule C18H26O5
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SMILES
OC1=CC=C(OC(C(CCCCC)CC(OC(C)C)=O)=O)C=C1
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Please store the product under the recommended conditions in the Certificate of Analysis.
Pureté et documentation
Références
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Anticancer agent 325 Related Classifications
HY-183714 Related Classifications
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