MK6-83
Based on 2 publication(s) in Google Scholar
MK6-83 is a new candidate agonist of TRPML1 with an improved efficacy and potency. MK6-83 has the potential for Mucolipidosis type IV study.
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- Pureté: 98.27%
- CAS No.: 1062271-24-2
- Formule: C16H20N2O2S2
- Masse moléculaire:336.47
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Stockage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Activité biologique
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Chemical Information
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Solvant et solubilité
- Pureté et documentation
- Références
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) MK6-83
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Customer Validation & Images
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IF
Activité biologique
Description
In Vitro
MK6-83 ranging from 0.2 to 30 μM shows no signs of cytotoxicity[1].
MK6-83 appears to be significantly more efficacious on fibroblast lysosomes isolated from R403C or V446L expressing cells than on those isolated from TRPML1-/- cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Lysosomes isolated from fibroblast cell lines derived from MLIV patients carrying either the F408D, the R403C or the V446L mutation[1].
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Concentration:0-10 μM.
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Incubation Time:24 h.
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Result:Efficacious on fibroblast lysosomes isolated from R403C or V446L expressing cells.
Had no significant effect on lysosomes isolated from TRPML1-/- fibroblasts.
Chemical Information
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CAS No. 1062271-24-2
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Appearance Solid
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Masse moléculaire 336.47
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Formule C16H20N2O2S2
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Color Light yellow to yellow
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SMILES
O=S(C1=CC=C(C)S1)(NC2=CC=CC=C2N3CCCCC3)=O
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Publications (2)
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Journal Impact Factor
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Most Recent
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Autophagy
2021 Dec;17(12):4401-4422. PMID: 33890549
MK6-83 purchased from MedChemExpress. Usage Cited in: Autophagy. 2021 Dec;17(12):4401-4422. [Abstract]
Another two MCOLN1 agonists, MK6-83 (5 μM) and ML-SA1 (20 μM), also led to significant increases in LC3-II levels in HeLa cells, respectively. Baf-A1 (1 μM) application did not further facilitate LC3-II increases caused by either MK6-83 or ML-SA1 treatments.
MK6-83 purchased from MedChemExpress. Usage Cited in: Autophagy. 2021 Dec;17(12):4401-4422. [Abstract]
Four hour treatments of 0.1 μM ML-SA5, 1 μM ML-SA5, 5 μM MK6-83 or 20 μM ML-SA1 caused increased abundance of LC3 puncta in GFP-LC3 stably expressing HEK 293 T (S-GFP-LC3) cell lines, respectively. Baf-A1 (1 μM) was a positive control for inducing LC3 puncta structures. LC3 puncta per cell under the control, ML-SA5 treatments (0.1 μM and 1 μM), MK6-83 (5 μM), ML-SA1 (20 μM) and Baf-A1 (1 μM) were quantified from 4–11 independent experiments (typically 10–40 cells).
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Solvant et solubilité
In Vitro:
DMSO : 25 mg/mL (74.30 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (7.43 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureté et documentation
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Fiche technique (275 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Instruction de manipulation (2659 KB)
Références
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 2.9720 mL | 14.8602 mL | 29.7203 mL | 74.3008 mL |
| 5 mM | 0.5944 mL | 2.9720 mL | 5.9441 mL | 14.8602 mL | |
| 10 mM | 0.2972 mL | 1.4860 mL | 2.9720 mL | 7.4301 mL | |
| 15 mM | 0.1981 mL | 0.9907 mL | 1.9814 mL | 4.9534 mL | |
| 20 mM | 0.1486 mL | 0.7430 mL | 1.4860 mL | 3.7150 mL | |
| 25 mM | 0.1189 mL | 0.5944 mL | 1.1888 mL | 2.9720 mL | |
| 30 mM | 0.0991 mL | 0.4953 mL | 0.9907 mL | 2.4767 mL | |
| 40 mM | 0.0743 mL | 0.3715 mL | 0.7430 mL | 1.8575 mL | |
| 50 mM | 0.0594 mL | 0.2972 mL | 0.5944 mL | 1.4860 mL | |
| 60 mM | 0.0495 mL | 0.2477 mL | 0.4953 mL | 1.2383 mL |
MK6-83 Related Classifications
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