Phenylpropiolic acid
Based on 1 Customer Validation
Phenylpropiolic acid is an Antibacterial agent. Phenylpropiolic acid is identified from the intracellular extract of Proteus mirabilis DMTMMR-11. Phenylpropiolic acid disrupts biofilm formation by reducing pathogen adhesion and enhancing biofilm clearance ability. Phenylpropiolic acid inhibits the growth of Yersinia enterocolitica, disrupts its cell membrane integrity, and increases the survival rate of infected Caenorhabditis elegans. Phenylpropiolic acid can be used in studies related to yersiniosis and gastroenteritis.
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- Pureté : 99.85%
- CAS No.: 637-44-5
- Formule: C9H6O2
- Masse moléculaire:146.15
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Stockage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Activité biologique
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Chemical Information
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Solvant et solubilité
- Pureté et documentation
- Références
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Help & FAQs
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Activité biologique
Description
IC50 & Target
[1]|
Microbial Metabolite |
In Vitro
Phenylpropiolic acid (50-200 μg/mL; 24 h) exhibits strong bacteriostatic activity against Yersinia enterocolitica (MTCC-840), with an MIC of 50 μg/mL, and inhibits bacterial growth over a 24 h period[1].
Phenylpropiolic acid (50 μg/mL; 2-12 h) inhibits biofilm formation, disrupts existing biofilms, and reduces metabolic activity of Yersinia enterocolitica (MTCC-840), achieving 65% biofilm inhibition after 12 h[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:N2 wild-type[1]
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Dosage:50 μg/mL
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Administration:pre-treatment; 1 hour
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Result:Reduced Yersinia enterocolitica-induced cellular damage in Caenorhabditis elegans.
Improved pharyngeal pumping rate between 24-48 hours post-treatment.
Lowered reactive oxygen species levels relative to untreated infected worms.
Enhanced worm survival compared to untreated infected worms with higher mortality after 72 hours.
Chemical Information
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CAS No. 637-44-5
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Appearance Solid
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Masse moléculaire 146.15
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Formule C9H6O2
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Color White to off-white
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SMILES
O=C(O)C#CC1=CC=CC=C1
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Structure Classification
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Initial Source
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvant et solubilité
In Vitro:
DMSO : 100 mg/mL (684.23 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (17.11 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (17.11 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureté et documentation
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Fiche technique (282 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Instruction de manipulation (2659 KB)
Références
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 6.8423 mL | 34.2114 mL | 68.4229 mL | 171.0571 mL |
| 5 mM | 1.3685 mL | 6.8423 mL | 13.6846 mL | 34.2114 mL | |
| 10 mM | 0.6842 mL | 3.4211 mL | 6.8423 mL | 17.1057 mL | |
| 15 mM | 0.4562 mL | 2.2808 mL | 4.5615 mL | 11.4038 mL | |
| 20 mM | 0.3421 mL | 1.7106 mL | 3.4211 mL | 8.5529 mL | |
| 25 mM | 0.2737 mL | 1.3685 mL | 2.7369 mL | 6.8423 mL | |
| 30 mM | 0.2281 mL | 1.1404 mL | 2.2808 mL | 5.7019 mL | |
| 40 mM | 0.1711 mL | 0.8553 mL | 1.7106 mL | 4.2764 mL | |
| 50 mM | 0.1368 mL | 0.6842 mL | 1.3685 mL | 3.4211 mL | |
| 60 mM | 0.1140 mL | 0.5702 mL | 1.1404 mL | 2.8510 mL | |
| 80 mM | 0.0855 mL | 0.4276 mL | 0.8553 mL | 2.1382 mL | |
| 100 mM | 0.0684 mL | 0.3421 mL | 0.6842 mL | 1.7106 mL |
Phenylpropiolic acid Related Classifications
HY-W007319 Related Classifications
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