Ro 31-9790
Based on 1 publication(s) in Google Scholar
Ro 31-9790 is a synthetic metalloproteinase (MMP) inhibitor.
Nur für Forschungszwecke. Wir verkaufen nicht an Patienten.
- Reinheit: 99.36%
- CAS. Nr.: 145337-55-9
- Formel: C15H29N3O4
- Molecular Weight:315.41
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Speicherung:
4°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
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Biologische Aktivität
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Chemical Information
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Lösungsmittel & Löslichkeit
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Publications Citing Use of MedChemExpress (MCE) Ro 31-9790
More
Biologische Aktivität
MMP[1]
Ro 31-9790 inhibits L-selectin shedding from mouse and human lymphocytes, Jurkat T cells, and human monocytes with an IC50 of 0.3-0.4 μM on these cell types. The IC50 values obtained for Ro 31-9790 are 4.82 ± 0.75 μM, 1.16 ± 0.27 μM, 0.70 ± 0.06 μM, 4.47± 1.27 μM and 0.38 ± 0.05 μM for mouse lymphocyte L-selectin shedding, Jurkat L-selectin shedding, human lymphocyte L-selectin shedding, human monocyte L-selectin shedding and human monocyte TNF-α shedding[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS. Nr. 145337-55-9
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Appearance Solid
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Molecular Weight 315.41
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Formel C15H29N3O4
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Color Off-white to light yellow
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SMILES
O=C(N[C@H](C(NC)=O)C(C)(C)C)[C@H](CC(C)C)CC(NO)=O
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Synonyms
GI4747
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
4°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
Publications (1)
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Journal Impact Factor
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Most Recent
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Nat Cell Biol
Trafficking circuit of CD8+ T cells between the intestine and bone marrow governs antitumour immunity. [Abstract]2024 Aug;26(8):1346-1358. PMID: 39039181
Lösungsmittel & Löslichkeit
DMSO : 50 mg/mL (158.52 mM; ultrasonic and warming and heat to 70°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
Protokoll
For mouse and human lymphocytes and Jurkat T cells, 100% inhibition is set as the percentage of cells positive for L-selectin in an untreated sample (after flow cytometric analysis) or the concentration of soluble L-selectin in the supernatant of an untreated cell sample (after ELISA); 0% inhibition is set at the appropriate value for a PMA-treated sample. For results from human monocytes, a different calculation is needed to allow direct comparison of L-selectin and TNF-α shedding. Therefore, in this case 100% inhibition is set as the percentage of cells positive for L-selectin in the presence of PMA+50 μM Ro 31-9790 or the percentage of cells positive for cell surface TNF-α in the presence of LPS+50 μM Ro 31-9790 (doses of Ro 31-9790 which gave maximal inhibition); 0% inhibition is set as the percentage of cells positive for L-selectin or TNF-α in the presence of PMA or LPS, respectively. Intermediate percent inhibition is calculated. The IC50 value for inhibitor in each system is defined as the concentration of inhibitor which gave 50% inhibition, where 100% inhibition is set as the percentage of cells positive for L-selectin or cell surface TNF-α in the presence of Phorbol myristate acetate (PMA)+50 μM Ro 31-9790 or Lipopolysaccharide (LPS)+50 μM Ro 31-9790, respectively (doses of Ro 31-9790 which gave maximal inhibition) and 0% inhibition is set as the percentage of cells positive for L-selectin or TNF-α in the presence of PMA or LPS alone, respectively[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Reinheit & Dokumentation
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Data Sheet (273 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
Verweise
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.1705 mL | 15.8524 mL | 31.7048 mL | 79.2619 mL |
| 5 mM | 0.6341 mL | 3.1705 mL | 6.3410 mL | 15.8524 mL | |
| 10 mM | 0.3170 mL | 1.5852 mL | 3.1705 mL | 7.9262 mL | |
| 15 mM | 0.2114 mL | 1.0568 mL | 2.1137 mL | 5.2841 mL | |
| 20 mM | 0.1585 mL | 0.7926 mL | 1.5852 mL | 3.9631 mL | |
| 25 mM | 0.1268 mL | 0.6341 mL | 1.2682 mL | 3.1705 mL | |
| 30 mM | 0.1057 mL | 0.5284 mL | 1.0568 mL | 2.6421 mL | |
| 40 mM | 0.0793 mL | 0.3963 mL | 0.7926 mL | 1.9815 mL | |
| 50 mM | 0.0634 mL | 0.3170 mL | 0.6341 mL | 1.5852 mL | |
| 60 mM | 0.0528 mL | 0.2642 mL | 0.5284 mL | 1.3210 mL | |
| 80 mM | 0.0396 mL | 0.1982 mL | 0.3963 mL | 0.9908 mL | |
| 100 mM | 0.0317 mL | 0.1585 mL | 0.3170 mL | 0.7926 mL |
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