Art. -Nr.: HY-149726

EZH2-IN-17

Name: EZH2-IN-17
Brand: MedChemExpress
SKU: HY-149726
Price: 400.00 USD
Availability: InStock
Rating: 5 (1 reviews)

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EZH2-IN-17 (compound 28) is a potent and orally active EZH2 inhibitor with an IC₅₀ value of 0.95 nM. EZH2-IN-17 exhibits high anti-proliferation activity against different lymphoma cell lines including WSU-DLCL2, Pfeiffer and Karpas-422 (IC50 values of 2.36 nM, 1.73 nM, and 1.82 nM, respectively).

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Reinheit: 99.35%
CAS. Nr.: 2489672-09-3
Formel: C₃₆H₄₆N₄O₄
Molecular Weight:598.77

Speicherung:

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

EZH2-IN-17 Chemical Structure

CAS. Nr.: 2489672-09-3

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Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
- Beschreibung
- Cellular Effect
Chemical Information
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

Alle Histone Methyltransferase Isoform-spezifische Produkte anzeigen :

Cellular Effect

Cell Line	Type	Value	Description	References
KARPAS-422	IC₅₀	1.82 nM Compound: 28	Antiproliferative activity against human KARPAS-422 cells harboring EZH2 Y641N mutant Antiproliferative activity against human KARPAS-422 cells harboring EZH2 Y641N mutant	[PMID: 37956762]
Pfeiffer	IC₅₀	1.73 nM Compound: 28	Antiproliferative activity against human Pfeiffer cells harboring EZH2 A677G mutant Antiproliferative activity against human Pfeiffer cells harboring EZH2 A677G mutant	[PMID: 37956762]
WSUDLCL2	IC₅₀	2.36 nM Compound: 28	Antiproliferative activity against human WSUDLCL2 cells harboring EZH2 Y641F mutant Antiproliferative activity against human WSUDLCL2 cells harboring EZH2 Y641F mutant	[PMID: 37956762]

Chemical Information

CAS. Nr. 2489672-09-3
Appearance Solid
Molecular Weight 598.77
Formel C₃₆H₄₆N₄O₄
Color White to off-white
SMILES

O=C1C(CNC(C2=CC(C3=CC=C(C(N4CCOCC4)CC5)C5=C3)=CC(N(C6CCOCC6)CC)=C2C)=O)=C(C)C=C(C)N1
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 100 mg/mL (167.01 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.6701 mL

8.3505 mL

16.7009 mL

5 mM

0.3340 mL

1.6701 mL

3.3402 mL

10 mM

0.1670 mL

0.8350 mL

1.6701 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (4.18 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (4.18 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (4.18 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (273 KB)
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Xin Xu, et al. Conformationally constrained potent inhibitors for enhancer of zeste homolog 2 (EZH2). Bioorg Med Chem Lett. 2023 Nov 11:129558. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	1.6701 mL	8.3505 mL	16.7009 mL	41.7523 mL
	5 mM	0.3340 mL	1.6701 mL	3.3402 mL	8.3505 mL
	10 mM	0.1670 mL	0.8350 mL	1.6701 mL	4.1752 mL
	15 mM	0.1113 mL	0.5567 mL	1.1134 mL	2.7835 mL
	20 mM	0.0835 mL	0.4175 mL	0.8350 mL	2.0876 mL
	25 mM	0.0668 mL	0.3340 mL	0.6680 mL	1.6701 mL
	30 mM	0.0557 mL	0.2783 mL	0.5567 mL	1.3917 mL
	40 mM	0.0418 mL	0.2088 mL	0.4175 mL	1.0438 mL
	50 mM	0.0334 mL	0.1670 mL	0.3340 mL	0.8350 mL
	60 mM	0.0278 mL	0.1392 mL	0.2783 mL	0.6959 mL
	80 mM	0.0209 mL	0.1044 mL	0.2088 mL	0.5219 mL
	100 mM	0.0167 mL	0.0835 mL	0.1670 mL	0.4175 mL

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EZH2-IN-17

Alle Histone Methyltransferase Isoform-spezifische Produkte anzeigen