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Art. -Nr.: HY-P991669

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Biologically Validated

IGN523

(Synonyms: AML-01)

Kundenbewertung

Based on 1 Customer Validation

IGN523 is an anti-CD98 antibody (hCD98, K_D = 0.55 nM). IGN523 induces antibody-dependent cell-mediated cytotoxicity (ADCC) activity, lysosomal membrane permeabilization, and inhibition of essential amino acid transport, ultimately leading to caspase-3 and caspase-7-mediated apoptosis of tumor cells. IGN523 inhibits tumor growth in multiple tumor xenograft models. IGN523 is useful in the research of non-small cell lung cancer (NSCLC), acute myeloid leukemia (AML), and other cancers..

Nur für Forschungszwecke. Wir verkaufen nicht an Patienten.

Reinheit: 97.47%
Molecular Weight:145.63 kDa

Speicherung:

Please store the product under the recommended conditions in the Certificate of Analysis.

IGN523 Chemical Structure

Größe

Preis

Verfügbarkeit

Menge

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
- Beschreibung
- Isotype
- Recommend Isotype Controls
- Species Reactivity
- IC₅₀ & Target
- In Vitro
- In Vivo
- Clinical Trial
- Gene ID
- Accession
- Conjugated
- Reconsititution
- Anwendung
- Verified Bioactivity
Chemical Information
Reinheit & Dokumentation
Verweise
Calculators
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

Isotype

Human IgG1 kappa

Recommend Isotype Controls

Human IgG1 kappa, Isotype Control

Species Reactivity

Human

IC₅₀ & Target^[1]

Caspase 3

Caspase-7

Alle Caspase Isoform-spezifische Produkte anzeigen :

In Vitro

IGN523 elicits strong ADCC activity in Ramos, KG-1 and OCI-AML-3 cells, with EC₅₀s of 18.6 ng/mL, 73.8 ng/mL and 83.3 ng/mL, respectively, does not reduce ADCC activity in IGN523 cells^[1].
IGN523 (2 µg/mL, 0.5-48 h) elicits Caspase-dependent apoptosis, increases lysosomal membrane permeability and decreases amino acid transport in Ramos, KG-1 and OCI-AML-3 cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Apoptosis Analysis^[1]

Cell Line:

Ramos, KG-1 and OCI-AML-3 cells
Concentration:

2 µg/mL
Incubation Time:

48 h
Result:

Decreased cell viability in the presence of AbXL (10 µg/mL) (Ramos: 70% reduction; OCI-AML-3: 76% reduction; KG-1: 80% reduction) , but not when used alone.
Increased caspase-3 and caspase-7 activity (Ramos: 3.3-fold; OCI-AML-3: 7.5-fold and KG-1: 6.6-fold).

Immunofluorescence^[1]

Cell Line:

Ramos cells
Concentration:

2 µg/mL
Incubation Time:

4℃ for 30 min
Result:

Showed the CD98-IGN523 complex localized to lysosome-associated membrane protein LAMP-1 structures, increased the rate of internalization, capped CD98 at the cell surface.

In Vivo

IGN523 (15 mg/kg, i.p., once a week, twice/three times) inhibits tumor growth in Ramos and Dau xenografts mice model^[1].
IGN523 (15 mg/kg, i.p., once a week, twice/three times/four times) inhibits tumor growth in AML and primary NSCLC xenograft mice models^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Ramos and Dau xenografts mice model^[1]
Dosage:

15 mg/kg
Administration:

i.p., once a week, twice/three times
Result:

Inhibited tumor growth, with TGIs of 98%, 76 %, respectively.

Animal Model:

AML (HL-60, OCI-AML-3 (NOD/SCID) and KG-1 (1 x 10⁷) and NSCLC (IGN-LNG-12 and IGN-LNG-54) (1 x 10⁶) xenografts NOD/SCID or CB.17-SCID mice model^[1]
Dosage:

15 mg/kg
Administration:

i.p., once a week, twice/three times/four times
Result:

Reduced tumor growth compared with isotype control in all 5 xenograft models, with TGIs of HL-60 (94%), OCI-AML-3 (74%), KG-1 (53%), IGN-LNG-12 (44%) and IGN-LNG-54 (56%), without inducing body weight loss. Completely eliminated CD98 expression and CD98⁺-tumor cells.

Clinical Trial

NCT Number	Sponsor	Condition	Start Date	Phase A549
NCT01329991	Plexxikon\|		2011-05	PHASE1

Gene ID

6520 [NCBI]

Accession

AAH01061

Conjugated

Unconjugated

Reconsititution

The product can be reconstituted/diluted with sterile PBS or saline.

Anwendung

ELISA, FACS, Functional assay

Verified Bioactivity

Immobilized Human CD98 Protein, hFC Tag can bind IGN523. The EC₅₀ for this effect is 80.8 ng/mL.

Chemical Information

Appearance Liquid
Molecular Weight 145.63 kDa
Color Colorless to light yellow
SMILES

N/A
Synonyms

AML-01
Versand

Shipping with dry ice.
Formulation

Please refer to the lot-specific COA for specific buffer information.
Speicherung

Please store the product under the recommended conditions in the Certificate of Analysis.

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (262 KB)
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Inhibitory Antibodies User Guide (603 KB)

Verweise

[1]. Hayes GM, Chinn L, Cantor JM, Cairns B, Levashova Z, Tran H, Velilla T, Duey D, Lippincott J, Zachwieja J, Ginsberg MH, H van der Horst E. Antitumor activity of an anti-CD98 antibody. Int J Cancer. 2015 Aug 1;137(3):710-20. [Content Brief]

Calculators

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Keywords

MOQ

Minimum order quantity

100 mg

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Other size

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Amount: USD 0.00

Biologische Aktivität
- Beschreibung
- Isotype
- Recommend Isotype Controls
- Species Reactivity
- IC₅₀ & Target
- In Vitro
- In Vivo
- Clinical Trial
- Gene ID
- Accession
- Conjugated
- Reconsititution
- Anwendung
- Biological Activity
Chemical Information
Reinheit & Dokumentation
Verweise

Produktname:
Art. -Nr.:
Menge:
MCE Japan Authorized Agent:

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CAS. Nr.:
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Reinheit:
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IGN523

Cytotoxicity LDH Assay Kit

PBS Buffer (1×)

DPBS Buffer (1×)

Antibody Inhibitor Library

Alle Caspase Isoform-spezifische Produkte anzeigen

Biologische Aktivität

Chemical Information

Reinheit & Dokumentation

Verweise

Calculators

Help & FAQs

IGN523 Related Classifications

HY-P991669 Related Classifications