Cat. No.: HY-N2127

Pinostrobin

3 Cited Publications

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Based on 3 publication(s) in Google Scholar

Pinostrobin is a flavonoid with anti-cancer, antioxidant, antiviral and neuroprotective activities. Pinostrobin has oral activity. Pinostrobin is a potent PCSK9 inhibitor that inhibits the catalytic activity of PCSK9. Pinostrobin can be used in the research of viral infections, cancer, leukemia, cardiovascular and cerebrovascular diseases, cirrhosis, inflammation and neurological diseases.

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Pureté: 99.98%
CAS No.: 480-37-5
Formule: C₁₆H₁₄O₄
Masse moléculaire:270.28

Stockage:

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Pinostrobin Chemical Structure

CAS No.: 480-37-5

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Activité biologique
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (3)
Solvant et solubilité
- In Vitro
- In Vivo
Pureté et documentation
Références
Help & FAQs
Classifications
Bioz

Activité biologique

Description

IC₅₀ & Target

HSV-1

TNF-α

IL-1β

PCSK9

Voir tous les produits spécifiques à Isoform HSV :

Voir tous les produits spécifiques à Isoform Interleukin Related :

Cellular Effect

Cell Line	Type	Value	Description	References
A549	EC₅₀	>370 μM Compound: 8	Antiproliferative activity against human A549 cells after 72 hrs by MTT assay Antiproliferative activity against human A549 cells after 72 hrs by MTT assay	[PMID: 12027739]
B16-BL6	EC₅₀	111 μM Compound: 8	Antiproliferative activity against mouse B16-BL6 cells after 72 hrs by MTT assay Antiproliferative activity against mouse B16-BL6 cells after 72 hrs by MTT assay	[PMID: 12027739]
COLO 320DM	IC₅₀	>100 μM Compound: 1	Cytotoxicity against human COLO320DM cells after 72 hrs by WST-8 assay Cytotoxicity against human COLO320DM cells after 72 hrs by WST-8 assay	[PMID: 20219370]
HeLa	EC₅₀	202 μM Compound: 8	Antiproliferative activity against human HeLa cells after 72 hrs by MTT assay Antiproliferative activity against human HeLa cells after 72 hrs by MTT assay	[PMID: 12027739]
HT-1080	EC₅₀	156 μM Compound: 8	Antiproliferative activity against human HT1080 cells after 72 hrs by MTT assay Antiproliferative activity against human HT1080 cells after 72 hrs by MTT assay	[PMID: 12027739]
MCF7	IC₅₀	>50 μM Compound: 11	Cytotoxicity against human MCF7 cells after 72 hrs by MTS reduction assay Cytotoxicity against human MCF7 cells after 72 hrs by MTS reduction assay	[PMID: 20704331]
MCF7	IC₅₀	31 μM Compound: 2	Inhibition of BCRP expressed in MCF-7 MX cells using Hoechst 33342 staining Inhibition of BCRP expressed in MCF-7 MX cells using Hoechst 33342 staining	[PMID: 21354800]
MCF7	IC₅₀	84.9 μM Compound: 1	Cytotoxicity against human MCF7 cells after 72 hrs by WST-8 assay Cytotoxicity against human MCF7 cells after 72 hrs by WST-8 assay	[PMID: 20219370]
MDCK	IC₅₀	8.9 μM Compound: 2	Inhibition of BCRP expressed in MDCK cells using Hoechst 33342 staining Inhibition of BCRP expressed in MDCK cells using Hoechst 33342 staining	[PMID: 21354800]
NCI-H460	IC₅₀	>50 μM Compound: 11	Cytotoxicity against human NCI-H460 cells after 72 hrs by MTS reduction assay Cytotoxicity against human NCI-H460 cells after 72 hrs by MTS reduction assay	[PMID: 20704331]
PC-3	IC₅₀	86.7 μM Compound: 1	Cytotoxicity against human PC3 cells after 72 hrs by WST-8 assay Cytotoxicity against human PC3 cells after 72 hrs by WST-8 assay	[PMID: 20219370]
RAW264.7	IC₅₀	53.5 μM Compound: 10	Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by Griess assay Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by Griess assay	[PMID: 23743282]
SF-268	IC₅₀	>50 μM Compound: 11	Cytotoxicity against human SF268 cells after 72 hrs by MTS reduction assay Cytotoxicity against human SF268 cells after 72 hrs by MTS reduction assay	[PMID: 20704331]
SK-BR-3	IC₅₀	94.3 μM Compound: 1	Cytotoxicity against human SK-BR-3 cells after 72 hrs by WST-8 assay Cytotoxicity against human SK-BR-3 cells after 72 hrs by WST-8 assay	[PMID: 20219370]

In Vitro

Pinostrobin (0-100 μg/mL, 72 h) inhibits HSV-1 virus, with the EC₅₀ value of 22.71 μg/mL^[2].
Pinostrobin (25-200 μM, 3 days) promotes melanin production in B16F10 cells by stimulating the expression of related melanin production regulatory factors in cAMP/PKA and p38 MAPK signaling pathways, and is non-toxic to cells^[5].
Pinostrobin (130 and 150 μM, 48 h) induces acute leukemia cell NB4 and MOLT-4 apoptosis via the regulation of miR-410-5p and SFRP5^[6].
Pinostrobin (0-200 μM, 24-96 h) effectively induces apoptosis through ROS-mediated mitochondrial damage of cervical cancer cells (HeLa, Ca Ski, SiHa), and is not toxic to non-cancer cells HEK293 ^[7].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[2]

Cell Line:

Vero cells
Concentration:

0-100 μg/mL
Incubation Time:

72 h
Result:

The maximum noncytotoxic concentration (TD₀) of pinostrobin was calculated as 95.37 ± 7.14μg/mL.

Western Blot Analysis^[5]

Cell Line:

B16F10 cell
Concentration:

25-200 μM
Incubation Time:

3 days
Result:

Promoted phosphorylation of related targets under the cascade reaction of cAMP/PKA and p38 MAPK signaling pathways, such as MITF, tyrosinase, trp1, creb, GSK-3β, β-catenin, ERK, p38, etc.

In Vivo

Pinostrobin (20 mg/kg , 50 mg/kg, i.g., daily for 7 days) delays the progression of lesions in a dose-dependent HSV-1 infected mouse model^[2].
Pinostrobin (Single oral 10 and 20 mg/kg) has an anti-inflammatory effect by inhibiting TNF-α and IL-1β levels in rat models of LPS-induced inflammation ^[3].
Pinostrobin (daily oral 30 and 60 mg/kg for 2 months) has liver protective effect on liver cirrhosis induced by Thioacetamide (TAA) (HY-Y0698) in rats^[4].

Relevant pharmacokinetic parameters of Pinostrobin in rats.^[8]

Route	Dose (mg/kg)	AUC_0-t (ng·min/mL)	MRT (h)	C_max (ng/mL)	T_max (h)	t_1/2 (min)
i.g.	0.5	3817.80	6.26	615.35	4	4.34

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Female Sprague Dawley (SD) rats (200–250 g), LPS (5 mg/kg) was administered after 2 h of the final dose. ^[3]
Dosage:

Single oral 10 and 20 mg/kg
Administration:

p.o.
Result:

Did not cause any signs of abnormal behavior and toxicity in the rats.
Dose-dependent resulted in significant reductions in the levels of two cytokines, TNF-α and IL-1β.

Animal Model:

healthy female male Sprague Dawley rats (6–7 weeks old, weighed between 180 and 210 g), Thioacetamide (HY-Y0698)-induced liver cirrhosis in rats^[4]
Dosage:

daily oral 30 and 60 mg/kg for 2 months, single oral 500mg/kg
Administration:

p.o.
Result:

Significantly reduced liver index and hepatocyte proliferation in rats, and significantly reduce cell damage.
Significantly down-regulated the expression levels of PCNA and α-SMA.
The activities of antioxidant enzymes (SOD) and CAT in liver homogenate were increased, and the level of malondialdehyde (MDA) was decreased. The levels of serum bilirubin, total protein, albumin and liver enzymes (ALP, ALT and AST) recovered to normal.
Decreased the levels of TNF-a and IL-6 and increased the levels of IL-10 in rats.
Acute toxicity with a higher dose of 500 mg/kg Pinostrobin did not manifest any toxicological signs in rats.

Chemical Information

CAS No. 480-37-5
Appearance Solid
Masse moléculaire 270.28
Formule C₁₆H₁₄O₄
Color White to yellow
SMILES

O=C1C[C@@H](C2=CC=CC=C2)OC3=CC(OC)=CC(O)=C13
Structure Classification
- Flavonoids
- Flavonones
- Phenols
- Monophenols
Initial Source
- Plants
- other families
Livraison

Room temperature in continental US; may vary elsewhere.
Stockage

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Publications (3)

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Journal Impact Factor

Naunyn Schmiedebergs Arch Pharmacol

Pinostrobin attenuates microglia-mediated neuroinflammation after subarachnoid hemorrhage through modulation of the MYC-CTSL signaling axis. [Abstract]

2026 Mar 11. PMID: 41807810
Vet Microbiol

The Chinese medicine monomer Schisandrin C inhibits PRRSV infection by regulating the OGT-PI3K/AKT/mTOR signaling pathway. [Abstract]

2026 May:316:110992. PMID: 41865607
Phytomed Plus

2023 Sep 1, 100483.

Solvant et solubilité

In Vitro:

DMSO : 100 mg/mL (369.99 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

3.6999 mL

18.4993 mL

36.9987 mL

5 mM

0.7400 mL

3.6999 mL

7.3997 mL

10 mM

0.3700 mL

1.8499 mL

3.6999 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Calculateur de molarité

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Calculateur de dilution

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (9.25 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (9.25 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Pureté et documentation

Select Batch:

Pureté: 99.98%

Assay: 99.98%

ee.: 99.98%

Fiche technique (284 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Instruction de manipulation (2659 KB)

Références

[1]. Gao WY, et al. Pinostrobin Inhibits Proprotein Convertase Subtilisin/Kexin-type 9 (PCSK9) Gene Expression through the Modulation of FoxO3a Protein in HepG2 Cells.J Agric Food Chem. 2018 Jun 20;66(24):6083-6093. [Content Brief]

[2]. Wu N, et al. Activity investigation of pinostrobin towards herpes simplex virus-1 as determined by atomic force microscopy. Phytomedicine. 2011 Jan 15;18(2-3):110-8.

[3]. Patel NK, et al. Pinostrobin and Cajanus lactone isolated from Cajanus cajan (L.) leaves inhibits TNF-α and IL-1β production: in vitro and in vivo experimentation. Phytomedicine. 2014 Jun 15;21(7):946-53. [Content Brief]

[4]. Shareef SH, et al. Hepatoprotective effect of pinostrobin against thioacetamide-induced liver cirrhosis in rats. Saudi J Biol Sci. 2023 Jan;30(1):103506. [Content Brief]

[5]. Yoon JH, et al. Discovery of Pinostrobin as a Melanogenic Agent in cAMP/PKA and p38 MAPK Signaling Pathway. Nutrients. 2022 Sep 9;14(18):3713. [Content Brief]

[6]. Norkaew C, et al. Pinostrobin induces acute leukemia cell apoptosis via the regulation of miR-410-5p and SFRP5. Life Sci. 2023 Jul 15;325:121739. [Content Brief]

[7]. Jaudan A, et al. Induction of apoptosis by pinostrobin in human cervical cancer cells: Possible mechanism of action. PLoS One. 2018 Feb 8;13(2):e0191523. [Content Brief]

[8]. Hua X, et al. Determination of pinostrobin in rat plasma by LC-MS/MS: application to pharmacokinetics. J Pharm Biomed Anal. 2011 Dec 5;56(4):841-5. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	3.6999 mL	18.4993 mL	36.9987 mL	92.4967 mL
	5 mM	0.7400 mL	3.6999 mL	7.3997 mL	18.4993 mL
	10 mM	0.3700 mL	1.8499 mL	3.6999 mL	9.2497 mL
	15 mM	0.2467 mL	1.2333 mL	2.4666 mL	6.1664 mL
	20 mM	0.1850 mL	0.9250 mL	1.8499 mL	4.6248 mL
	25 mM	0.1480 mL	0.7400 mL	1.4799 mL	3.6999 mL
	30 mM	0.1233 mL	0.6166 mL	1.2333 mL	3.0832 mL
	40 mM	0.0925 mL	0.4625 mL	0.9250 mL	2.3124 mL
	50 mM	0.0740 mL	0.3700 mL	0.7400 mL	1.8499 mL
	60 mM	0.0617 mL	0.3083 mL	0.6166 mL	1.5416 mL
	80 mM	0.0462 mL	0.2312 mL	0.4625 mL	1.1562 mL
	100 mM	0.0370 mL	0.1850 mL	0.3700 mL	0.9250 mL

Keywords

Pinostrobin

3 Cited Publications

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Pinostrobin

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Publications Citing Use of MedChemExpress (MCE) Pinostrobin

Voir tous les produits spécifiques à Isoform HSV

Voir tous les produits spécifiques à Isoform Interleukin Related

Activité biologique

Chemical Information

Publications (3)

Solvant et solubilité

Pureté et documentation

Références

Help & FAQs

Complete Stock Solution Preparation Table

Pinostrobin Related Classifications

Cat. No.: HY-N2127