Cat. No.: HY-110117

BIRT 377

4 Cited Publications

Customer Review

Based on 4 publication(s) in Google Scholar

BIRT 377 is a potent amd orally bioavailable inhibitor of the interaction between intercellular adhesion molecule-1 (ICAM-1) and lymphocyte function-associated antigen-1 (LFA-1). BIRT 377 also inhibits the production of IL-2 in vivo. BIRT 377 can be used for researching inflammatory and immune disorders.

For research use only. We do not sell to patients.

Purity: 99.82%
CAS No.: 213211-10-0
Formula: C₁₈H₁₅BrCl₂N₂O₂
Molecular Weight:442.13

Storage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

BIRT 377 Chemical Structure

CAS No.: 213211-10-0

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (4)
Solvent & Solubility
- In Vitro
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target

IL-2

All Integrin Isoforms :

All Interleukin Related Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
H1-HeLa	IC₅₀	26 nM Compound: BIRT-377	Inhibitory concentration against leukocyte function-associated antigen-1 in HSB-2 human lymphoblast and H1HeLa cell adhesion assay Inhibitory concentration against leukocyte function-associated antigen-1 in HSB-2 human lymphoblast and H1HeLa cell adhesion assay	[PMID: 15686933]
JY	IC₅₀	0.24 μM Compound: 1	Concentration required to inhibit aggregation of JY cells Concentration required to inhibit aggregation of JY cells	[PMID: 15481974]

In Vitro

BIRT 377 (20 μM, 6 h) inhibits the mRNA and protein expression of EMT-related markers ZEB2 and Vementin in HT-29 cell whem cocultured with LAD2 cell^[2].
BIRT 377 (20 μM, 24 h) inhibits the cell migration of HT-29, when cocultured with LAD2^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[2]

Cell Line:

HT-29
Concentration:

20 μM
Incubation Time:

6 h
Result:

Inhibited the protein expression of ZEB2 and Vementin.

In Vivo

BIRT 377 (3-10 mg/kg, po, once daily for 14 days) inhibits the production of human IgG in hPBMC-injected mouse models, with an effect comparable to that of anti-LFA-1 antibodies^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

hPBMC-injected mouse models^[3]
Dosage:

3-10 mg/kg
Administration:

po, once daily for 14 days
Result:

Inhibited the production of human IgG.

Chemical Information

CAS No. 213211-10-0
Appearance Solid
Molecular Weight 442.13
Formula C₁₈H₁₅BrCl₂N₂O₂
Color White to off-white
SMILES

O=C1N(C2=CC(Cl)=CC(Cl)=C2)C(N(C)[C@]1(C)CC3=CC=C(Br)C=C3)=O
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Publications (4)

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Anal Chem

Exposome-Scale Investigation of Cl-/Br-Containing Chemicals Using High-Resolution Mass Spectrometry, Multistage Machine Learning, and Cloud Computing. [Abstract]

2025 Jun 3;97(21):11099-11109. PMID: 40401576
PNAS Nexus

Low-affinity LFA1-dependent outside-in signaling mediates avidity modulation via the Rabin8-Rab8 axis. [Abstract]

2024 Aug 8;3(8):pgae332. PMID: 39170909
bioRxiv

Mast cells interact directly with colorectal cancer cells to promote epithelial-to-mesenchymal transition. [Abstract]

2025 Mar 19:2025.03.19.644113. PMID: 40166179
Res Sq

2024 Jun 04.

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (226.18 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.2618 mL

11.3089 mL

22.6178 mL

5 mM

0.4524 mL

2.2618 mL

4.5236 mL

10 mM

0.2262 mL

1.1309 mL

2.2618 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (276 KB)
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Kelly TA, et al. Cutting edge: a small molecule antagonist of LFA-1-mediated cell adhesion. J Immunol. 1999;163(10):5173-5177. [Content Brief]

[2]. Lanzloth R, et al. Mast cells interact directly with colorectal cancer cells to promote epithelial-to-mesenchymal transition[J]. bioRxiv, 2025: 2025.03. 19.644113.

[3]. Winquist RJ, et al., The role of leukocyte function-associated antigen-1 in animal models of inflammation. Eur J Pharmacol. 2001 Oct 19;429(1-3):297-302. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.2618 mL	11.3089 mL	22.6178 mL	56.5445 mL
	5 mM	0.4524 mL	2.2618 mL	4.5236 mL	11.3089 mL
	10 mM	0.2262 mL	1.1309 mL	2.2618 mL	5.6544 mL
	15 mM	0.1508 mL	0.7539 mL	1.5079 mL	3.7696 mL
	20 mM	0.1131 mL	0.5654 mL	1.1309 mL	2.8272 mL
	25 mM	0.0905 mL	0.4524 mL	0.9047 mL	2.2618 mL
	30 mM	0.0754 mL	0.3770 mL	0.7539 mL	1.8848 mL
	40 mM	0.0565 mL	0.2827 mL	0.5654 mL	1.4136 mL
	50 mM	0.0452 mL	0.2262 mL	0.4524 mL	1.1309 mL
	60 mM	0.0377 mL	0.1885 mL	0.3770 mL	0.9424 mL
	80 mM	0.0283 mL	0.1414 mL	0.2827 mL	0.7068 mL
	100 mM	0.0226 mL	0.1131 mL	0.2262 mL	0.5654 mL

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BIRT 377

Immunology/Inflammation Compound Library

Covalent Screening Library

Differentiation Inducing Compound Library

Cytoskeleton Compound Library

Orally Active Compound Library

Angiogenesis-Related Compound Library

Heterocyclic Compound Library

Membrane Protein-targeted Compound Library

Cytokine Inhibitors Library

Extracellular Vesicles (EVs) Compound Library

Radioprotector Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Covalent Screening Library Plus

Bioactive Compound Library

Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

High-Throughput Bioactive Compound Library

Publications Citing Use of MedChemExpress (MCE) BIRT 377

All Integrin Isoforms

All Interleukin Related Isoforms

Biological Activity

Chemical Information

Publications (4)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

BIRT 377

Immunology/Inflammation Compound Library

Covalent Screening Library

Differentiation Inducing Compound Library

Cytoskeleton Compound Library

Orally Active Compound Library

Angiogenesis-Related Compound Library

Heterocyclic Compound Library

Membrane Protein-targeted Compound Library

Cytokine Inhibitors Library

Extracellular Vesicles (EVs) Compound Library

Radioprotector Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Covalent Screening Library Plus

Bioactive Compound Library

Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

High-Throughput Bioactive Compound Library

Publications Citing Use of MedChemExpress (MCE) BIRT 377

All Integrin Isoforms

All Interleukin Related Isoforms

Biological Activity

Chemical Information

Publications (4)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

BIRT 377 Related Classifications

HY-110117 Related Classifications