Apecotrep
Based on 1 Customer Validation
Apecotrep (TRPC6-IN-3) (compound 17) is a potent, orally active transient receptor potential C6 ion channel (TRPC6) inhibitor. Apecotrep modulates not only intracellular calcium concentration, but also membrane potential by modulating the flux of cations including calcium and sodium ions. Apecotrep can be used in research of respiratory system.
Para uso exclusivo en investigación. No vendemos a pacientes.
- Pureza: 99.17%
- No. CAS: 2311863-36-0
- Fòrmula: C22H22FN5O3
- Peso molecular:423.44
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Almacenamiento:Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Pureza y Documentación
- Referencias
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Help & FAQs
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Immunology/Inflammation Compound Library
HY-L007
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Membrane Transporter/Ion Channel Compound Library
HY-L011
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Neuronal Signaling Compound Library
HY-L013
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Orally Active Compound Library
HY-L061
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Mechanoreceptors Compound Library
HY-L095
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Heterocyclic Compound Library
HY-L138
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Pain-Related Compound Library
HY-L139
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Membrane Protein-targeted Compound Library
HY-L149
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Membrane Receptor-targeted Compound Library
HY-L150
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Ion Channel Compound Library
HY-L166
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Bioactive Compound Library Max
HY-L181
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MCE Bioactive Compound Library
HY-L001V
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Bioactive Compound Library
HY-L001
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High-Throughput Bioactive Compound Library
HY-L205
Actividad biológica
Apecotrep (3 mg/kg; p.o.; daily, for 4 d; H1N1-induced mice) reduces H1N1-induced vascular leakage in a mouse model[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:LPS-induced mice[1]
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Dosage:1, 3, and 10 mg/kg
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Administration:Oral administration; orally 12 h and 2 h before LPS challenge
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Result:Reduced BALF protein concentration of 56 % at 3 mg/kg and 62 % at 10 mg/kg.
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Animal Model:H1N1-induced mice[1]
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Dosage:3 mg/kg
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Administration:Oral administration; daily, for 4 days
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Result:Inhibited Evans blue extravasation from the blood to the BALF and reduced BALF Evans blue by 24 % at 3 mg/kg.
Chemical Information
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No. CAS 2311863-36-0
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Appearance Solid
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Peso molecular 423.44
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Fòrmula C22H22FN5O3
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Color White to off-white
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SMILES
O=C(N1CCC(C2=NN=C(N)C=C2)CC1)C3=NC=C(OC4=CC=C(F)C=C4)C(OC)=C3
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Synonyms
TRPC6-IN-3
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Powder -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Solvente y solubilidad
DMSO : 125 mg/mL (295.20 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (4.91 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (4.91 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureza y Documentación
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Ficha de datos (270 KB)
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SDS (419 KB)
- English - EN (419 KB)
- Français - FR (419 KB)
- Deutsch - DE (419 KB)
- Norwegian - NO (419 KB)
- Español - ES (419 KB)
- Swedish - SV (419 KB)
- Italian - IT (419 KB)
- Korean - KR (419 KB)
- Portuguese - PT (419 KB)
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Instrucciones de manejo (2659 KB)
Referencias
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.3616 mL | 11.8080 mL | 23.6161 mL | 59.0402 mL |
| 5 mM | 0.4723 mL | 2.3616 mL | 4.7232 mL | 11.8080 mL | |
| 10 mM | 0.2362 mL | 1.1808 mL | 2.3616 mL | 5.9040 mL | |
| 15 mM | 0.1574 mL | 0.7872 mL | 1.5744 mL | 3.9360 mL | |
| 20 mM | 0.1181 mL | 0.5904 mL | 1.1808 mL | 2.9520 mL | |
| 25 mM | 0.0945 mL | 0.4723 mL | 0.9446 mL | 2.3616 mL | |
| 30 mM | 0.0787 mL | 0.3936 mL | 0.7872 mL | 1.9680 mL | |
| 40 mM | 0.0590 mL | 0.2952 mL | 0.5904 mL | 1.4760 mL | |
| 50 mM | 0.0472 mL | 0.2362 mL | 0.4723 mL | 1.1808 mL | |
| 60 mM | 0.0394 mL | 0.1968 mL | 0.3936 mL | 0.9840 mL | |
| 80 mM | 0.0295 mL | 0.1476 mL | 0.2952 mL | 0.7380 mL | |
| 100 mM | 0.0236 mL | 0.1181 mL | 0.2362 mL | 0.5904 mL |
Apecotrep Related Classifications
HY-148129 Related Classifications
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