Spermine
Based on 10 publication(s) in Google Scholar
Spermine is a natural antioxidant and anti-inflammatory agent. Spermine is known to inhibit some bacterial cultures, especially strains of Staphylococcus aureus. Spermine can reversibly inhibits DNA synthetic response, mixed lymphocyte response and the induction of cytolytic lymphocyte response in primary cultures of murine spleen cells. Spermine is a polyamine nitric oxide donor that can provide nitric oxide to platelets and inhibit platelet activation to a certain extent concentration-dependently. Spermine occurs in mammalian tissues, plants, bacteria, ribosomes and bacteriophage. Spermine inhibits primary human embryo lung fibroblasts in vitro.
Para uso exclusivo en investigación. No vendemos a pacientes.
- Pureza: 99.79%
- No. CAS: 71-44-3
- Fòrmula: C10H26N4
- Peso molecular:202.34
-
Almacenamiento:
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Actividad biológica
-
Chemical Information
-
Solvente y solubilidad
- Pureza y Documentación
- Referencias
-
Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Spermine
More- Cell. 2026 Jan 22;189(2):418-434.e24. [Abstract]
- Nat Commun. 2025 Dec 8. [Abstract]
- Basic Res Cardiol. 2021 Dec 16;116(1):65. [Abstract]
- Chin Med. 2026 Jun 10;21(1):167. [Abstract]
- Acta Physiol. 2023 Apr;237(4):e13926. [Abstract]
- J Biol Chem. 2024 Nov;300(11):107862. [Abstract]
- PLoS Genet. 2026 Jan 23;22(1):e1012013. [Abstract]
- Virus Res. 2022 Apr 15:312:198708. [Abstract]
- Vet Microbiol. 2026 May:316:110992. [Abstract]
- Res Sq. 2026 May 18.
Customer Validation & Images
-
Cell Imaging/Staining
-
Bio/Physico-chemical Assay
-
Bio/Physico-chemical Assay
-
In Vivo Efficacy Study
-
WB
Ver todos los productos específicos de isoformas Endogenous Metabolite
MoreVer todos los productos específicos de isoformas DNA/RNA Synthesis
More
Actividad biológica
|
Microbial Metabolite |
Human Endogenous Metabolite |
|
Cell Line
|
Type | Value | Description | References |
|---|---|---|---|---|
| CHO | IC50 |
>100 μM
Compound: Spm, spermine
|
Cytotoxicity against CHO cells after 48 hrs by MTT assay
Cytotoxicity against CHO cells after 48 hrs by MTT assay
|
[PMID: 18558490] |
| CHO | IC50 |
>100 μM
Compound: Spm, spermine
|
Cytotoxicity against polyamine transport-deficient mutant CHO cells after 48 hrs by MTT assay
Cytotoxicity against polyamine transport-deficient mutant CHO cells after 48 hrs by MTT assay
|
[PMID: 18558490] |
| CHO-MG | IC50 |
>100 μM
Compound: 3, spermine
|
Inhibition of cell growth in CHO-MG cells in the presence of DFMO
Inhibition of cell growth in CHO-MG cells in the presence of DFMO
|
[PMID: 16392808] |
| Fibroblast | IC50 |
>500 μM
Compound: Spermine
|
Cytotoxicity against human wild type CMS-24949 cells in presence of aminoguanidine measured after 72 hrs
Cytotoxicity against human wild type CMS-24949 cells in presence of aminoguanidine measured after 72 hrs
|
[PMID: 34695351] |
| Fibroblast | IC50 |
>500 μM
Compound: Spermine
|
Cytotoxicity against SMS mutant human CMS-23916 cells in presence of aminoguanidine measured after 72 hrs
Cytotoxicity against SMS mutant human CMS-23916 cells in presence of aminoguanidine measured after 72 hrs
|
[PMID: 34695351] |
| Fibroblast | IC50 |
14.6 μM
Compound: Spermine
|
Cytotoxicity against SMS mutant human CMS-26559 cells in absence of aminoguanidine measured after 72 hrs
Cytotoxicity against SMS mutant human CMS-26559 cells in absence of aminoguanidine measured after 72 hrs
|
[PMID: 34695351] |
| Fibroblast | IC50 |
14.9 μM
Compound: Spermine
|
Cytotoxicity against SMS mutant human CMS-23916 cells in absence of aminoguanidine measured after 72 hrs
Cytotoxicity against SMS mutant human CMS-23916 cells in absence of aminoguanidine measured after 72 hrs
|
[PMID: 34695351] |
| Fibroblast | IC50 |
356.7 μM
Compound: Spermine
|
Cytotoxicity against SMS mutant human CMS-26559 cells in presence of aminoguanidine measured after 72 hrs
Cytotoxicity against SMS mutant human CMS-26559 cells in presence of aminoguanidine measured after 72 hrs
|
[PMID: 34695351] |
| Fibroblast | IC50 |
422.1 μM
Compound: Spermine
|
Cytotoxicity against SMS mutant human CMS-6233 cells in presence of aminoguanidine measured after 72 hrs
Cytotoxicity against SMS mutant human CMS-6233 cells in presence of aminoguanidine measured after 72 hrs
|
[PMID: 34695351] |
| HeLa | IC50 |
50 μM
Compound: spermine
|
Ability to inhibit 50% growth of HeLa cells
Ability to inhibit 50% growth of HeLa cells
|
[PMID: 1875339] |
| HeLa | IC50 |
70 μM
Compound: spermine
|
Ability to cause aggregation of purified HeLa cell DNA
Ability to cause aggregation of purified HeLa cell DNA
|
[PMID: 1875339] |
| Huh-7 | CC50 |
>50 μM
Compound: Spermine
|
Cytotoxicity against human HuH7 cells
Cytotoxicity against human HuH7 cells
|
[PMID: 24177358] |
| Huh-7 | EC50 |
>50 μM
Compound: Spermine
|
Antiviral activity against cell culture derived HCV expressing NS5A-GFP fusion protein infected in human HuH7 cells assessed as inhibition of viral life cycle after 72 hrs by Hoechst-33342 staining-based confocal microscopy
Antiviral activity against cell culture derived HCV expressing NS5A-GFP fusion protein infected in human HuH7 cells assessed as inhibition of viral life cycle after 72 hrs by Hoechst-33342 staining-based confocal microscopy
|
[PMID: 24177358] |
| L1210 | IC50 |
0.39 μM
Compound: Spermine
|
Compound was tested for inhibitory concentration required to inhibit 50% of [14C]- putrescine uptake.
Compound was tested for inhibitory concentration required to inhibit 50% of [14C]- putrescine uptake.
|
[PMID: 9871574] |
| L1210 | IC50 |
54.5 μM
Compound: Spm, spermine
|
Cytotoxicity against mouse L1210 ATCC CCL 219 cells after 48 hrs by MTT assay
Cytotoxicity against mouse L1210 ATCC CCL 219 cells after 48 hrs by MTT assay
|
[PMID: 18558490] |
Spermine (40-100 μg/mL, 5 d) has ID50 of 25 and 35 in H.E.L. cells and H.Ep.2 cells, respectively[2].
Spermine (10 μg/well, when added up to 29 h after inception of culture) can inhibit the bacterial Lipopolysaccharide (HY-D1056) in BALB/c spleen cells[3].
Spermine (5-10 μg/well, when added within 22 h after inception of culture) can completely inhibit concanavalin A (HY-P2149) in BALB/c spleen cells[3].
Spermine (0.5 mM) shows 50% inhibition of peroxidation reaction in brain phospholipid liposomes[4].
Spermine tetrahydrochloride (10-50 mM) increases the concentration of NO in platelets and inhibits the expression of CD62p concentration-dependently[5].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
| NCT Number | Sponsor | Condition | Start Date |
Phase
|
|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
Chemical Information
-
No. CAS 71-44-3
-
Appearance <28°C Solid,>30°C Liquid
-
Peso molecular 202.34
-
Fòrmula C10H26N4
-
Color Colorless to off-white
-
SMILES
NCCCNCCCCNCCCN
-
Synonyms
NSC 268508; Neuridine
-
Structure Classification
-
Initial Source
-
Envío
Room temperature in continental US; may vary elsewhere.
-
Almacenamiento
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Publications (10)
-
Journal Impact Factor
-
Most Recent
-
Cell
Tumor-produced ammonia is metabolized by regulatory T cells to further impede anti-tumor immunity. [Abstract]2026 Jan 22;189(2):418-434.e24. PMID: 41448182 -
Nat Commun
2025 Dec 8. PMID: 41361241
Spermine purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Dec 8. [Abstract]
Immunofluorescence showed the expression of JunD in basal cells before and within 9 days after irradiation (Spermine, 10 mg/kg, ip, 16 days).
-
Basic Res Cardiol
Protective effect of HINT2 on mitochondrial function via repressing MCU complex activation attenuates cardiac microvascular ischemia-reperfusion injury. [Abstract]2021 Dec 16;116(1):65. PMID: 34914018
Spermine purchased from MedChemExpress. Usage Cited in: Basic Res Cardiol. 2021 Dec 16;116(1):65. [Abstract]
Spermine (5 mg/kg, ip, single dose) treatment failed to alleviate no-reflow phenomenon and enhance microvascular perfusion.
Spermine purchased from MedChemExpress. Usage Cited in: Basic Res Cardiol. 2021 Dec 16;116(1):65. [Abstract]
The beneficial effects of HINT2 on nitrite levels, eNOS expression, and eNOS phosphorylation were completely suppressed after Spermine (10 μM, 2 h) treatment.
-
Chin Med
Heterophyllin B ameliorates diabetic lower limb ischemia by inhibiting SMOX to activate the Nrf2 antioxidant pathway. [Abstract]2026 Jun 10;21(1):167. PMID: 42271493 -
Acta Physiol
2023 Apr;237(4):e13926. PMID: 36606511 -
J Biol Chem
Different charged biopolymers induce α-synuclein to form fibrils with distinct structures. [Abstract]2024 Nov;300(11):107862. PMID: 39374778
Spermine purchased from MedChemExpress. Usage Cited in: J Biol Chem. 2024 Nov;300(11):107862. [Abstract]
ThT kinetics of α-synuclein aggregation in the presence of different concentrations of Spermine (0-10 mM).
Spermine purchased from MedChemExpress. Usage Cited in: J Biol Chem. 2024 Nov;300(11):107862. [Abstract]
Statistical analysis was performed on the maximum fluorescence intensity (Fmax) of the kinetic curve after 99 hours of Spermine (3.2-16 μM) incubation.
-
PLoS Genet
A C. elegans model of copper deficiency: Dietary interventions rescue CTR1/CHCA-1 copper transporter mutant phenotype. [Abstract]2026 Jan 23;22(1):e1012013. PMID: 41575937 -
Virus Res
PEDV infection affects the expression of polyamine-related genes inhibiting viral proliferation. [Abstract]2022 Apr 15:312:198708. PMID: 35151773 -
Vet Microbiol
The Chinese medicine monomer Schisandrin C inhibits PRRSV infection by regulating the OGT-PI3K/AKT/mTOR signaling pathway. [Abstract]2026 May:316:110992. PMID: 41865607 -
Solvente y solubilidad
H2O : ≥ 200 mg/mL (988.44 mM)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Pureza y Documentación
-
Ficha de datos (274 KB)
-
SDS (478 KB)
- English - EN (478 KB)
- Français - FR (478 KB)
- Deutsch - DE (478 KB)
- Norwegian - NO (478 KB)
- Español - ES (478 KB)
- Swedish - SV (478 KB)
- Italian - IT (478 KB)
- Korean - KR (478 KB)
- Portuguese - PT (478 KB)
-
Instrucciones de manejo (2659 KB)
Referencias
[2]. Dawson, M., & Dryden, W. F. (1969). The toxicity of spermine and spermidine to cells in culture. Biochemical pharmacology, 18(6), 1307–1313. [Content Brief]
[3]. Byrd, W. J., et al., (1977). Synthetic polyamines added to cultures containing bovine sera reversibly inhibit in vitro parameters of immunity. Nature, 267(5612), 621–623. [Content Brief]
[4]. Løvaas, E., & Carlin, G. (1991). Spermine: an anti-oxidant and anti-inflammatory agent. Free radical biology & medicine, 11(5), 455–461. [Content Brief]
[6]. Otsuki, M., et al., (1995). In vivo pharmacological study of spermine-induced neurotoxicity. Neuroscience letters, 196(1-2), 81–84. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 4.9422 mL | 24.7109 mL | 49.4218 mL | 123.5544 mL |
| 5 mM | 0.9884 mL | 4.9422 mL | 9.8844 mL | 24.7109 mL | |
| 10 mM | 0.4942 mL | 2.4711 mL | 4.9422 mL | 12.3554 mL | |
| 15 mM | 0.3295 mL | 1.6474 mL | 3.2948 mL | 8.2370 mL | |
| 20 mM | 0.2471 mL | 1.2355 mL | 2.4711 mL | 6.1777 mL | |
| 25 mM | 0.1977 mL | 0.9884 mL | 1.9769 mL | 4.9422 mL | |
| 30 mM | 0.1647 mL | 0.8237 mL | 1.6474 mL | 4.1185 mL | |
| 40 mM | 0.1236 mL | 0.6178 mL | 1.2355 mL | 3.0889 mL | |
| 50 mM | 0.0988 mL | 0.4942 mL | 0.9884 mL | 2.4711 mL | |
| 60 mM | 0.0824 mL | 0.4118 mL | 0.8237 mL | 2.0592 mL | |
| 80 mM | 0.0618 mL | 0.3089 mL | 0.6178 mL | 1.5444 mL | |
| 100 mM | 0.0494 mL | 0.2471 mL | 0.4942 mL | 1.2355 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Spermine Related Classifications
HY-B1777 Related Classifications
Powered by Bioz