DNA Stain
There are several different stains that can be used to visualize and photograph DNA after separation by gel electrophoresis. Molecules of the dye adhere to DNA strands and fluoresce under UV light, showing where the bands are within the gel.
Hoechst stains are part of a family of blue fluorescent dyes used to stain DNA.There are three related Hoechst stains: Hoechst 33258, Hoechst 33342, and Hoechst 34580. The dyes Hoechst 33258 and Hoechst 33342 are the ones most commonly used and they have similar excitation/emission spectra.
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DNA Stain Related Products (88)
Related Products (88)
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DAPI dihydrochloride
0 ImagesSynonyms: 4',6-Diamidino-2-phenylindole dihydrochlorideDAPI (4',6-Diamidino-2-phenylindole) dihydrochloride is a DAPI dye. DAPI is a fluorescent dye that binds strongly to DNA. It binds to the AT base pair of the double-stranded DNA minor groove, and one DAPI molecule can occupy three base pair positions. The fluorescence intensity of DAPI molecules bound to double-stranded DNA is increased by about 20 times, and it is commonly observed with fluorescence microscopy, and the amount of DNA can be determined based on the intensity of fluorescence. DAPI cannot penetrate intact cell membranes and is commonly used for staining damaged and fixed cells. DAPI (Compound 3) is an acid-sensing ion channel 3 (ASIC3) inhibitor. DAPI binds to ASIC3 and blocks the channel function. DAPI can be used in the study of chronic pain treatment (Ex/Em = 356/451 nm).
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EthD bromide
0 ImagesEthD bromide is an intercalating agent commonly used as a fluorescent tag (nucleic acid stain) in molecular biology laboratories for techniques such as agarose gel electrophoresis.
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Hoechst 33258
0 ImagesSynonyms: bisBenzimide H 33258; H 33258Hoechst 33258 is a blue to blue-green fluorescent live cell dye that can label DNA. Hoechst 33258 can specifically bind to the minor groove of DNA (and tends to bind to A/T-rich DNA), resulting in a significant increase in fluorescence intensity. Hoechst 33258 can cross the cell membrane and cause changes in DNA structure, such as G2/M phase arrest. Hoechst 33258 can bind to live or fixed cells, and the fluorescence intensity increases with increasing solution pH. As a DNA-specific probe, Hoechst 33258 can be used to detect DNA content, analyze cell cycle, etc. The excitation wavelength of Hoechst 33258 is 350-365 nm, and the emission wavelength is 460-490 nm.
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Cy3-dCTP
0 ImagesCat. No.: HY-D1725Cy3-dCTP is a directly fluorescently labeled deoxyribonucleotide, in which Cy3 is a cyanine fluorescent dye. Cy3-dCTP is used for direct enzymatic labeling of DNA and cDNA: with the aid of DNA polymerases, this modified nucleotide is incorporated into the extending DNA strand during processes such as reverse transcription, PCR, nick translation or random primer labeling.
The product specifications provided below refer solely to the active content of Cy3-dCTP.
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DeepRed Nucleus Dye
0 ImagesCat. No.: HY-D1742DeepRed Nucleus Dye is a novel cell permeant and far red-fluorescing DNA probe. DeepRed Nucleus Dye excites at a wavelength of 647 nm, close to the Ex, and produces a fluorescence spectrum extending from 665 nm out to beyond 780 nm wavelengths. DeepRed Nucleus Dye fluorescence reflects cellular DNA content. DeepRed Nucleus Dye can be used in combination with FITC and RPE-labelled antibodies, without the need for fluorescence compensation.
The product specifications provided below indicate the total amount only; they do not represent the actual content of DeepRed Nucleus Dye.
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Biotin-11-dCTP
0 ImagesCat. No.: HY-D1668Purity: 99.1%Biotin-11-dCTP is a biotinylated deoxynucleoside triphosphate and an important DNA labeling reagent. In random primer DNA labeling reactions, Biotin-11-dCTP incorporates into newly synthesized DNA strands to generate labeled DNA probes suitable for hybridization applications. In addition, Biotin-11-dCTP can serve as a substrate for terminal deoxynucleotidyl transferase to end-label oligonucleotides for telomere sequence detection, or to label the cut ends of linearized DNA molecules, thereby supporting streptavidin-based electron microscopy analysis. For example, Biotin-11-dCTP can label the cut ends of linearized DNA molecules under the action of dGTP and avian myeloblastosis virus reverse transcriptase.
The product specifications provided below refer solely to the active content of Biotin-11-dCTP.
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Biotin-aniline
0 ImagesBiotin-aniline is a probe with substantially high reactivity towards RNA and DNA. Biotin-aniline is also a novel APEX2 substrate. Biotin-aniline can label proteins via miniSOG. Biotin-aniline emerges as more efficient probe for capturing subcellular transcriptome in living cells with high spatial specificity.
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7-Aminoactinomycin D
0 Images7-Aminoactinomycin D (7-AAD) a cell-impermeant fluorescent DNA stain, is a potent RNA polymerase inhibitor. 7-Aminoactinomycin D selectively binds to GC regions of the DNA. 7-Aminoactinomycin D also has antibacterial effects.
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CY5-N3
0 ImagesSynonyms: Sulfo-Cyanine5-azideCY5-N3 (Sulfo-Cyanine5-azide) is a Cy5-azide, which is a fluorescent dye (ex/em: 646/662 nm). CY5-N3 is cell membrane permeable and can be used for live cell imaging. CY5-N3 can be used in cell imagine by Click reaction.
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Pyronin Y
0 ImagesPyronin Y (Pyronine G) is a cationic dye that intercalates RNA and has been used to target cell structures including RNA, DNA and organelles. Pyronin Y forms fluorescent complexes with double-stranded nucleic acids (especially RNA) enabling semi-quantitative analysis of cellular RNA. Pyronin Y can be used to identify specific RNA subspecies of ribonuclear proteins complexes in live cells.
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Quinacrine
0 ImagesQuinacrine (Acriquine) is an antimalarial and anti-cancer agent. Quinacrine also inhibits human aldehyde oxidase (IC50: 3.3 μM). Quinacrine has affinity for nucleic acids, and stains DNA and RNA in fixed cells (Ex/Em: 436/525 nm).
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DAPI
0 ImagesSynonyms: 4',6-Diamidino-2-phenylindoleDAPI (4',6-Diamidino-2-phenylindole) is a DAPI dye. DAPI is a fluorescent dye that binds strongly to DNA. It binds to the AT base pair of the double-stranded DNA minor groove, and one DAPI molecule can occupy three base pair positions. The fluorescence intensity of DAPI molecules bound to double-stranded DNA is increased by about 20 times, and it is commonly observed with fluorescence microscopy, and the amount of DNA can be determined based on the intensity of fluorescence. DAPI cannot penetrate intact cell membranes and is commonly used for staining damaged and fixed cells. DAPI (Compound 3) is an acid-sensing ion channel 3 (ASIC3) inhibitor. DAPI binds to ASIC3 and blocks the channel function. DAPI can be used in the study of chronic pain treatment (Ex/Em = 356/451 nm).
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CellTracker Blue CMF2HC Dye
0 ImagesCellTracker Blue CMF2HC Dye is a blue dye, can be used in two-channel nuclei acid sequencing, with blue and purple excitation light (450-460 nm/400-405nm or 415-450 nm/480-525nm). CellTracker Blue CMF2HC Dye can be used to rapid determination of antibiotic sensitivity of microorganisms.
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2-Aminopurine
0 Images2-Aminopurine, a fluorescent analog of guanosine and adenosine, is a widely used fluorescence-decay-based probe of DNA structure. When 2-Aminopurine is inserted in anoligonucleotide, its fluorescence is highly quenched by stacking with the natural bases. 2-Aminopurine has been used to probe nucleic acid structure and dynamics.
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MeCY5-NHS ester triethylamine
0 ImagesCat. No.: HY-135413BSynonyms: Sulfo-Cyanine5 NHS ester triethylamineMeCY5-NHS ester (potassium) is a reactive dye and can be used for labeling protein nucleic acid.
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Azure A chloride
0 ImagesAzure A chloride is a phenothiazine dye. Azure A chloride is an alternative DNA dye used for the separation of DNA and protein fragments in agarose gel electrophoresis and PAGE. Azure A chloride can be chemisorbed on the surface of mild steel according to the Langmuir adsorption isotherm to form a protective film. Azure A chloride binds to double-stranded DNA in a non-cooperative manner via weak intercalation, triggering molecular conformational disturbance, restricted rotational motion, and changes in optical activity.
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Methyl Green
0 ImagesCat. No.: HY-D0163CAS No.: 14855-76-6Methyl Green is a non-intercalating fluorescent labeling agent that selectively binds to the major groove of DNA. Methyl Green electrostatically interacts with the major groove of DNA through positively charged groups, exhibiting key activities such as high affinity, resistance to photobleaching, and stable fluorescence emission. Methyl Green can be directly measured by microscopy and flow cytometry, with peaks at 633 and 677 nm. Methyl Green can be used for fluorescent labeling of the nuclei of embryonic tissues or cells, or DNA staining and cell activity detection in gel electrophoresis[1][2][3].
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G-quadruplex DNA fluorescence probe 1
0 ImagesCat. No.: HY-147652G-quadruplex DNA fluorescence probe 1 (Compound E1) is a selective G-quadruplex DNA targeting fluorescent probe. G-quadruplex DNA fluorescence probe 1 can pass through membrane and enter living cells with low cytotoxicity.
