Anticancer agent 325
Anticancer agent 325 is an apoptosis/autophagy inducer and anticancer agent. Anticancer agent 325 exhibits cytotoxic activity against gastric cancer cells, with no significant effect on healthy gastric epithelial cells. Anticancer agent 325 enhances the expression of activated/cleaved Caspase-3 to promote apoptosis. Anticancer agent 325 also enhances the cleavage of PARP, a downstream marker of apoptosis, promotes LC3B lipidation, and activates autophagy. Anticancer agent 325 can be used in the research of gastric cancer.
商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- CAS 番号: 2922798-57-8
- 分子式: C18H26O5
- 分子量:322.40
-
保管条件:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
生物活性
-
化学情報
- プロトコル
- 純度とドキュメンテーション
- 参考文献
-
Help & FAQs
Caspase アイソフォーム固有の製品をすべて表示
More
生物活性
製品説明
IC50 & Target
[1]|
Caspase 3 |
体外実験
Anticancer agent 325 (compound 2d) (1.9-120.0 μM; 72 h) potently induces cytotoxicity in AGS and KATO III gastric cancer cells, with IC50 values of 30.9 μM and 19.0 μM, respectively; by contrast, it shows a higher IC50 value of 38.3 μM in non-malignant GES-1 gastric epithelial cells[1].
Anticancer agent 325 (30 μM; 24 h) modulates apoptosis and autophagy markers in AGS and KATO III gastric cancer cells, but exerts no such effect on non-cancerous GES-1 gastric epithelial cells[1].
Anticancer agent 325 (30 μM; 24 h) induces morphological changes of apoptosis and autophagosome formation in AGS gastric cancer cells, while it induces necrotic/apoptotic changes and autophagosome formation in KATO III gastric cancer cells, and also causes mitochondrial damage[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Cell Line:human gastric adenocarcinoma AGS cells, human signet ring cell carcinoma KATO III cells, noncancerous gastric epithelial immortalized GES-1 cells
-
Concentration:1.9, 3.8, 7.5, 15.0, 30.0, 60.0, 120.0 μM
-
Incubation Time:72 h
-
Result:Potently reduced viability of AGS cells with an IC50 of 30.9 μM.
Potently reduced viability of KATO III cells with an IC50 of 19.0 μM.
Reduced viability of GES-1 cells with an IC50 of 38.3 μM.
Induced dose-dependent cytotoxicity, with significant reductions in cell viability at multiple concentrations relative to untreated controls.
- RNA extraction experimental
- Detection of Apoptosis
- TUNEL staining for apoptotic DNA fragmentation
- Cell Cytotoxicity Assay
- Annexin V plus membrane-impermeant dye apoptosis staining
- Autophagy
- Apoptosis Solutions
- Lysosome and acidic-vesicle live-cell staining
- Macroautophagy Solutions
- Cell Viability Determination by MTT Colorimetric Assay
化学情報
-
CAS 番号 2922798-57-8
-
分子量 322.40
-
分子式 C18H26O5
-
SMILES
OC1=CC=C(OC(C(CCCCC)CC(OC(C)C)=O)=O)C=C1
-
輸送条件
Room temperature in continental US; may vary elsewhere.
-
保管条件
Please store the product under the recommended conditions in the Certificate of Analysis.
純度とドキュメンテーション
参考文献
Calculators
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
Anticancer agent 325 関連分類
HY-183714 関連分類
Powered by Bioz