Harmine (hydrochloride)

Harmine hydrochloride purchased from MedChemExpress. Usage Cited in: J Clin Invest. 2024 Jul 18;134(18):e179472. [Abstract]

Customer Validation
Western blotting images showing the expression of ECM-related genes in primary human intestinal fibroblasts with or without TGF-β (5 ng/mL, 48 hours) and Harmine administration (5 μM or 10 μM, 48 hours).

Harmine hydrochloride purchased from MedChemExpress. Usage Cited in: J Clin Invest. 2024 Jul 18;134(18):e179472. [Abstract]

Customer Validation
Masson’s trichrome staining showing collagen deposition in mouse colons across the 4 indicated groups. Twist1^fl/fl mice and Col1a2-Cre^ERT2Twist1^fl/fl cohoused littermates (8 weeks old) were injected intraperitoneally with tamoxifen daily for 4 days (100 mg per kg body weight each time). The mice were subjected to 3 cycles of DSS administration (7 days of DSS administration followed by 14 days of regular drinking water). Harmine (10 mg/kg) was injected intraperitoneally twice a week during each DSS cycle (regularly on Tuesday and Friday weekly).

Harmine hydrochloride purchased from MedChemExpress. Usage Cited in: J Clin Invest. 2024 Jul 18;134(18):e179472. [Abstract]

Customer Validation
Representative IF staining of mouse colons across the 4 indicated groups (original magnification, ×20). DAPI (blue), COL1A1 (red), and vimentin (green) in merged channels are shown. Twist1^fl/fl mice and Col1a2-Cre^ERT2Twist1^fl/fl cohoused littermates (8 weeks old) were injected intraperitoneally with tamoxifen daily for 4 days (100 mg per kg body weight each time). The mice were subjected to 3 cycles of DSS administration (7 days of DSS administration followed by 14 days of regular drinking water). Harmine (10 mg/kg) was injected intraperitoneally twice a week during each DSS cycle (regularly on Tuesday and Friday weekly).

Harmine hydrochloride purchased from MedChemExpress. Usage Cited in: J Biomed Sci. 2022 Jun 2;29(1):34. [Abstract]

Customer Validation
HepG2 cells were incubated with Harmine (0, 2, 4, 8 μM) and were then cultured in the upper compartment of Transwell chambers for 24 h. Then, the cells were stained with crystal violet solution.

Harmine hydrochloride purchased from MedChemExpress. Usage Cited in: J Biomed Sci. 2022 Jun 2;29(1):34. [Abstract]

Customer Validation
Following treatment with Harmine (0, 5, 10, 20 μM) for 24 h at the indicated concentrations, total protein was extracted from HEPG2 and Hep3B cells and analysed by western blotting.

Harmine hydrochloride purchased from MedChemExpress. Usage Cited in: J Biomed Sci. 2022 Jun 2;29(1):34. [Abstract]

Customer Validation
HCC cells were serum-starved overnight, a scratch was created, and the cells were then incubated with 10 μM harmine and/or 10 ng/mL TGF-β for 6 h. Images were acquired under a microscope.

Harmine hydrochloride purchased from MedChemExpress. Usage Cited in: J Biomed Sci. 2022 Jun 2;29(1):34. [Abstract]

Customer Validation
Cells were serum-starved overnight and were then treated with Harmine (10 μM) or 10 ng/mL TGF-β for 6 h. Western blot analysis was then performed.

Harmine hydrochloride purchased from MedChemExpress. Usage Cited in: Prog Neuropsychopharmacol Biol Psychiatry. 2017 Oct 3;79(Pt B):258-267. [Abstract]

Customer Validation
Representative images showing the restoration effect of Harmine on CUS-induced decrease in hippocampal DCX protein expressions. The Fluoxetine administration (20 mg/kg) is used as a positive control, and all data are shown as mean±SEM.