IGN523
Based on 1 Customer Validation
IGN523 is an anti-CD98 antibody (hCD98, KD = 0.55 nM). IGN523 induces antibody-dependent cell-mediated cytotoxicity (ADCC) activity, lysosomal membrane permeabilization, and inhibition of essential amino acid transport, ultimately leading to caspase-3 and caspase-7-mediated apoptosis of tumor cells. IGN523 inhibits tumor growth in multiple tumor xenograft models. IGN523 is useful in the research of non-small cell lung cancer (NSCLC), acute myeloid leukemia (AML), and other cancers..
商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- 純度: 97.47%
- 分子量:145.63 kDa
-
保管条件:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
生物活性
-
化学情報
- 純度とドキュメンテーション
- 参考文献
-
Help & FAQs
Caspase アイソフォーム固有の製品をすべて表示
More
生物活性
Human IgG1 kappa
Human
|
Caspase 3 |
Caspase-7 |
IGN523 elicits strong ADCC activity in Ramos, KG-1 and OCI-AML-3 cells, with EC50s of 18.6 ng/mL, 73.8 ng/mL and 83.3 ng/mL, respectively, does not reduce ADCC activity in IGN523 cells[1].
IGN523 (2 µg/mL, 0.5-48 h) elicits Caspase-dependent apoptosis, increases lysosomal membrane permeability and decreases amino acid transport in Ramos, KG-1 and OCI-AML-3 cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Cell Line:Ramos, KG-1 and OCI-AML-3 cells
-
Concentration:2 µg/mL
-
Incubation Time:48 h
-
Result:Decreased cell viability in the presence of AbXL (10 µg/mL) (Ramos: 70% reduction; OCI-AML-3: 76% reduction; KG-1: 80% reduction) , but not when used alone.
Increased caspase-3 and caspase-7 activity (Ramos: 3.3-fold; OCI-AML-3: 7.5-fold and KG-1: 6.6-fold).
-
Cell Line:Ramos cells
-
Concentration:2 µg/mL
-
Incubation Time:4℃ for 30 min
-
Result:Showed the CD98-IGN523 complex localized to lysosome-associated membrane protein LAMP-1 structures, increased the rate of internalization, capped CD98 at the cell surface.
IGN523 (15 mg/kg, i.p., once a week, twice/three times/four times) inhibits tumor growth in AML and primary NSCLC xenograft mice models[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Animal Model:Ramos and Dau xenografts mice model[1]
-
Dosage:15 mg/kg
-
Administration:i.p., once a week, twice/three times
-
Result:Inhibited tumor growth, with TGIs of 98%, 76 %, respectively.
-
Animal Model:AML (HL-60, OCI-AML-3 (NOD/SCID) and KG-1 (1 x 107) and NSCLC (IGN-LNG-12 and IGN-LNG-54) (1 x 106) xenografts NOD/SCID or CB.17-SCID mice model[1]
-
Dosage:15 mg/kg
-
Administration:i.p., once a week, twice/three times/four times
-
Result:Reduced tumor growth compared with isotype control in all 5 xenograft models, with TGIs of HL-60 (94%), OCI-AML-3 (74%), KG-1 (53%), IGN-LNG-12 (44%) and IGN-LNG-54 (56%), without inducing body weight loss. Completely eliminated CD98 expression and CD98+-tumor cells.
| NCT Number | Sponsor | Condition | Start Date |
Phase
|
|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
AAH01061
Unconjugated
The product can be reconstituted/diluted with sterile PBS or saline.
ELISA, FACS, Functional assay
-
Immobilized Human CD98 Protein, hFC Tag can bind IGN523. The EC50 for this effect is 80.8 ng/mL.
化学情報
-
性状 Liquid
-
分子量 145.63 kDa
-
Color Colorless to light yellow
-
SMILES
N/A
-
別名
AML-01
-
輸送条件
Shipping with dry ice.
-
Formulation
Please refer to the lot-specific COA for specific buffer information.
-
保管条件
Please store the product under the recommended conditions in the Certificate of Analysis.
純度とドキュメンテーション
-
データシート (262 KB)
-
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
-
Inhibitory Antibodies User Guide (603 KB)
参考文献
Calculators
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
IGN523 関連分類
HY-P991669 関連分類
Powered by Bioz