製品番号: HY-N0142

フロレチン

(別名: Phloretin; NSC 407292; RJC 02792)

25 Cited Publications

カスタマーレビュー

Based on 25 publication(s) in Google Scholar

Phloretin (NSC 407292; RJC 02792) is a flavonoid extracted from Malus pumila Mill.，has anti-inflammatory activities. Phloridzin is a specific，competitive and orally active inhibitor of sodium/glucose cotransporters in the intestine (SGLT1) and kidney (SGLT2). Phloretin inhibits Yeast-made GLUT1 as well as Human erythrocyte GLUT1 with IC₅₀values of 49 μM and 61 μM，respectively.Phloretin has the potential for the treatment of rheumatoid arthritis (RA) and allergic airway inflammation.

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純度: 99.92%
CAS 番号: 60-82-2
分子式: C₁₅H₁₄O₅
分子量:274.27

保管条件:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 1 year , -20°C, 6 months

Phloretin 構造式

CAS 番号: 60-82-2

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生物活性
- 製品説明
- IC₅₀ & Target
- Cellular Effect
- 体外実験
- 体内実験
化学情報
Publications (25)
溶剤 & 溶解度
- 体外
- 体内
純度とドキュメンテーション
参考文献
Help & FAQs
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生物活性

製品説明

Phloretin (NSC 407292; RJC 02792) is a flavonoid extracted from Malus pumila Mill.，has anti-inflammatory activities. Phloridzin is a specific，competitive and orally active inhibitor of sodium/glucose cotransporters in the intestine (SGLT1) and kidney (SGLT2). Phloretin inhibits Yeast-made GLUT1 as well as Human erythrocyte GLUT1 with IC₅₀values of 49 μM and 61 μM，respectively^[1].Phloretin has the potential for the treatment of rheumatoid arthritis (RA) and allergic airway inflammation^[4].

IC₅₀ & Target^[1]

SGLT1

SGLT2

Microbial Metabolite

GLUT1

GLUT2

SGLT アイソフォーム固有の製品をすべて表示 :

SGLT1
SGLT2

Endogenous Metabolite アイソフォーム固有の製品をすべて表示 :

GLUT アイソフォーム固有の製品をすべて表示 :

Cellular Effect

Cell Line	Type	Value	Description	References
HEK293	IC₅₀	12.4 μM Compound: 9h	Inhibition of human SGLT1 expressed in HEK293 cells assessed as reduction in 2-deoxyglucose uptake pretreated for 10 mins followed by 2-deoxyglucose addition in presence of sodium buffer measured after 1 hr by resazurin dye based fluorescence assay Inhibition of human SGLT1 expressed in HEK293 cells assessed as reduction in 2-deoxyglucose uptake pretreated for 10 mins followed by 2-deoxyglucose addition in presence of sodium buffer measured after 1 hr by resazurin dye based fluorescence assay	[PMID: 28098449]
HEK293	IC₅₀	18.3 μM Compound: 9h	Inhibition of human SGLT2 expressed in HEK293 cells assessed as reduction in 2-deoxyglucose uptake pretreated for 10 mins followed by 2-deoxyglucose addition in presence of sodium buffer measured after 1 hr by resazurin dye based fluorescence assay Inhibition of human SGLT2 expressed in HEK293 cells assessed as reduction in 2-deoxyglucose uptake pretreated for 10 mins followed by 2-deoxyglucose addition in presence of sodium buffer measured after 1 hr by resazurin dye based fluorescence assay	[PMID: 28098449]
HEK293	IC₅₀	90 μM Compound: 4788	Inhibition of the Sodium/Hydrogen Exchanger-Like Domain-Containing Protein 2 (NHA2, SLC9B2) as assessed by a sensor pH-sensitive genetically encoded fluorescent sensor (pHAxensor) in HEK-293 cells stably transfected with SLC9B2 Inhibition of the Sodium/Hydrogen Exchanger-Like Domain-Containing Protein 2 (NHA2, SLC9B2) as assessed by a sensor pH-sensitive genetically encoded fluorescent sensor (pHAxensor) in HEK-293 cells stably transfected with SLC9B2	10.5281/zenodo.7360521
HEK293	IC₅₀	90 μM Compound: 4788	Inhibition of the Sodium/Hydrogen Exchanger-Like Domain-Containing Protein 2 (NHA2, SLC9B2) as assessed by a sensor pH-sensitive genetically encoded fluorescent sensor (pHAxensor) in HEK-293 cells stably transfected with SLC9B2 (PubChem AID: 1794821) Inhibition of the Sodium/Hydrogen Exchanger-Like Domain-Containing Protein 2 (NHA2, SLC9B2) as assessed by a sensor pH-sensitive genetically encoded fluorescent sensor (pHAxensor) in HEK-293 cells stably transfected with SLC9B2 (PubChem AID: 1794821)	10.5281/zenodo.7360521
K562	IC₅₀	41 μM Compound: Phloretin	Inhibition of NF-kappaB transactivation in TNF-alpha-stimulated human K562 cells preincubated for 2 hrs followed by TNF-alpha challenge measured after 6 hrs by dual luciferase reporter gene assay Inhibition of NF-kappaB transactivation in TNF-alpha-stimulated human K562 cells preincubated for 2 hrs followed by TNF-alpha challenge measured after 6 hrs by dual luciferase reporter gene assay	[PMID: 24775915]
MCF7	IC₅₀	>10 μM Compound: 5b	Cytotoxicity against human MCF7 cells assessed as reduction in cell viability after 72 hrs by MTT assay Cytotoxicity against human MCF7 cells assessed as reduction in cell viability after 72 hrs by MTT assay	[PMID: 26690274]
MCF7	IC₅₀	7 μM Compound: Phloretin	Antiproliferative activity against human MCF7 cells assessed as inhibition of cell growth incubated for 6 days by SRB assay Antiproliferative activity against human MCF7 cells assessed as inhibition of cell growth incubated for 6 days by SRB assay	[PMID: 33257172]
MDA-MB-231	IC₅₀	>10 μM Compound: 5b	Cytotoxicity against human MDA-MB-231 cells assessed as reduction in cell viability after 72 hrs by MTT assay Cytotoxicity against human MDA-MB-231 cells assessed as reduction in cell viability after 72 hrs by MTT assay	[PMID: 26690274]
NCI-H1299	IC₅₀	54 μM Compound: Phloretin	Cytotoxicity against human H1299 cells assessed as growth inhibition after 48 hrs by MTT assay Cytotoxicity against human H1299 cells assessed as growth inhibition after 48 hrs by MTT assay	[PMID: 24200808]
Oocyte	IC₅₀	41 μM Compound: Phloretin	TP_TRANSPORTER: inhibition of L-Lactate uptake (L-Lactate:30mM) in Xenopus laevis oocytes TP_TRANSPORTER: inhibition of L-Lactate uptake (L-Lactate:30mM) in Xenopus laevis oocytes	[PMID: 11101640]
Vero	CC₅₀	735.47 μg/mL Compound: 31	Cytotoxicity against african green monkey Vero cells assessed as cell death after 72 hrs by MTT assay Cytotoxicity against african green monkey Vero cells assessed as cell death after 72 hrs by MTT assay	[PMID: 22169259]
Vero	IC₅₀	125 μg/mL Compound: 31	Cytotoxicity against African green monkey Vero cells assessed as inhibition of cell growth after 72 hrs by MTT assay Cytotoxicity against African green monkey Vero cells assessed as inhibition of cell growth after 72 hrs by MTT assay	[PMID: 22169259]

体外実験

Phloretin induced obvious cytotoxicity against BEL-7402 cells with IC₅₀ of 89.23 μM^[2].
Phloretin (40-160 μM; 24 hours) induces BEL-7402 cell apoptosis though the mitochondrial pathway，the cells exposed to phloretin exhibits nuclear chromatin condensation and increased fluorescence intensity. The caspase-9 reaches the peak level at 12 h，and leak levels of caspase-6 and caspase-3 activities occurrs 18 and 24 h after the exposure，respectively^[2].
Phloretin (0-100 μM; 24 hours) has effects on transcription factors of adipogenesis in differentiated 3T3-L1 adipocytes，decreases PPAR-γ，C/EBPα and C/EBPβ as a dose-dependent manner^[3].
Phloretin (0-100 μM; 24 hours) has effects on the AMPK pathway in differentiated 3T3-L1 adipocytes，increases the phosphorylation of substrate ACC-1，AMPK in PT-treated cells^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Apoptosis Analysis^[2]

Cell Line:

BEL-7402 cell
Concentration:

40-160 μM
Incubation Time:

24 hours
Result:

Induced cell apoptosis and activated caspase 3, 6 and 9.

Western Blot Analysis^[3]

Cell Line:

3T3-L1 adipocytes
Concentration:

0 μM, 3 μM, 10 μM, 30 μM, 100 μM
Incubation Time:

24 hours
Result:

Inhibited PPAR-γ, C/EBPα and C/EBPβ expression.

Western Blot Analysis^[3]

Cell Line:

3T3-L1 adipocytes
Concentration:

0 μM, 3 μM, 10 μM, 30 μM, 100 μM
Incubation Time:

24 hours
Result:

Upregulated the expression of p-ACC-1, p-AMPK, p-AMPKα and β .

体内実験

Phloretin (oral administration; 50 or 100 mg/kg; once daily) decreases oxidative stress and diminished levels of malondialdehyde (MDA) and hydrogen peroxide in paw tissue，reduces productivity of anti-collagen antibodies in serum^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Collagen-Induced Arthritis (CIA) Mice^[3]
Dosage:

50 or 100 mg/kg
Administration:

Oral adminstration
Result:

Showed mitigation of clinical symptoms of RA in addition to reduced inflammation of hind-limbs compared to the control group.

化学情報

CAS 番号 60-82-2
性状 Solid
分子量 274.27
分子式 C₁₅H₁₄O₅
Color Off-white to pink
SMILES

O=C(C1=C(O)C=C(O)C=C1O)CCC2=CC=C(O)C=C2
別名

Phloretin; NSC 407292; RJC 02792
Structure Classification
- Flavonoids
- Dihydrochalcones
- Phenols
- Polyphenols
Initial Source
- Endogenous metabolite
- Human Gut Microbiota Metabolites
- Microorganisms
輸送条件

Room temperature in continental US; may vary elsewhere.

保管条件

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	1 year
	-20°C	6 months

Publications (25)

All Cell Imaging/StainingFlow CytometryBio/Physico-chemical Assay

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Journal Impact Factor

Nat Immunol

Microbiota-shaped neutrophil senescence regulates sexual dimorphism in bladder cancer. [Abstract]

2025 Apr 11. PMID: 40217111
Cell Res

The metabolite α-KG induces GSDMC-dependent pyroptosis through death receptor 6-activated caspase-8. [Abstract]

2021 Sep;31(9):980-997. PMID: 34012073
Acta Pharm Sin B

Chrysin serves as a novel inhibitor of DGK α/FAK interaction to suppress the malignancy of esophageal squamous cell carcinoma (ESCC). [Abstract]

2021 Jan;11(1):143-155. PMID: 33532186

Phloretin purchased from MedChemExpress. Usage Cited in: Acta Pharm Sin B. 2021 Jan;11(1):143-155. [Abstract]

Effect of Phloretin (50 μM) or lonidamine (50 μM) on DM-αKG-induced pyroptosis. HeLa cells were pretreated with phloretin or lonidamine for 2 hours.

Phloretin purchased from MedChemExpress. Usage Cited in: Acta Pharm Sin B. 2021 Jan;11(1):143-155. [Abstract]

Effect of Phloretin (50 μM) or lonidamine (50 μM) on DM-αKG induced intracellular pH. HeLa cells were pretreated with phloretin or lonidamine for 2 hours.

Phloretin purchased from MedChemExpress. Usage Cited in: Acta Pharm Sin B. 2021 Jan;11(1):143-155. [Abstract]

Effect of Phloretin (50 μM) or lonidamine (50 μM) on DM-αKG induced L-2HG levels. HeLa cells were pretreated with phloretin or lonidamine for 2 hours.
Exp Mol Med

Simultaneous treatment with sorafenib and glucose restriction inhibits hepatocellular carcinoma in vitro and in vivo by impairing SIAH1-mediated mitophagy. [Abstract]

2022 Nov;54(11):2007-2021. PMID: 36385558

Phloretin purchased from MedChemExpress. Usage Cited in: Exp Mol Med. 2022 Nov;54(11):2007-2021. [Abstract]

Huh7 cells were treated with sorafenib (5 μM) and/or Phloretin (50 and 100 μM) for 24 h.

Phloretin purchased from MedChemExpress. Usage Cited in: Exp Mol Med. 2022 Nov;54(11):2007-2021. [Abstract]

Huh7 cells were pretreated with1μMinsulin to activate glucose transport, and then treated with Canagliflozin (20 μM) or Phloretin(100 μM) for 1 hour before measurement of glucose uptake.
J Hazard Mater

In vitro and in vivo investigations on arsenic-induced cartilage degeneration in osteoarthritis. [Abstract]

2024 Jan 5:461:132570. PMID: 37742380
Food Chem

Effects of sun drying combined with baking processes on the flavor quality of Chongqing Tuocha raw tea. [Abstract]

2025 Dec 30:497:146992. PMID: 41285060
Food Chem

Flavonoid-mediated metabolic underpinning quality variation in red bud-sport pear mutants. [Abstract]

2025 May 31:489:144992. PMID: 40466530
Phytomedicine

Protective effects of E Se tea extracts against alcoholic fatty liver disease induced by high fat/alcohol diet: In vivo biological evaluation and molecular docking study. [Abstract]

2022 Jul:101:154113. PMID: 35490493
Ind Crops Prod

2025 Dec 11;239:122458.
J Agric Food Chem

High-Pressure and Thermal Pasteurization Applied to Smoothies Enhances (Poly)Phenol Bioaccessibility along the Gastrointestinal Tract. [Abstract]

2025 Jun 25;73(25):15561-15578. PMID: 40497562
Food Funct

Phloretin attenuation of hepatic steatosis via an improvement of mitochondrial dysfunction by activating AMPK-dependent signaling pathways in C57BL/6J mice and HepG2 cells. [Abstract]

2021 Dec 13;12(24):12421-12433. PMID: 34788781
Foods

Design of a Plant-Based Smoothie: Exploiting Ingredient Complementarity for a Diversified (Poly)phenolic Profile Quantified by Targeted LC-MS/MS Analysis. [Abstract]

2026 Apr 9;15(8):1293. PMID: 42073181
J Drug Deliv Sci Technol

2025 Dec.
Int J Mol Sci

Inhibition of NAMPT by PAK4 Inhibitors. [Abstract]

2024 Sep 21;25(18):10138. PMID: 39337621
Int Immunopharmacol

Natural compound phloretin restores periodontal immune homeostasis via HIF-1α-regulated PI3K/Akt and glycolysis in macrophages. [Abstract]

2024 Aug 25:141:112933. PMID: 39186834
Int Immunopharmacol

LncRNA-mRNA co-expression analysis reveals aquaporin-9-promoted neutrophil extracellular trap formation and inflammatory activation in sepsis. [Abstract]

2024 Aug 11:140:112916. PMID: 39133961
Int Immunopharmacol

The inhibition of GLUT1-induced glycolysis in macrophage by phloretin participates in the protection during acute lung injury. [Abstract]

2022 Sep:110:109049. PMID: 35853279
RSC Adv

Dual inhibitory potential of ganoderic acid A on GLUT1/3: computational and in vitro insights into targeting glucose metabolism in human lung cancer. [Abstract]

2024 Sep 6;14(39):28569-28584. PMID: 39247503
Cell Signal

The oncogenic role of TFAP2A in bladder urothelial carcinoma via a novel long noncoding RNA TPRG1-AS1/DNMT3A/CRTAC1 axis. [Abstract]

2023 Feb:102:110527. PMID: 36410635
J Nat Prod

Target Separation and Potential Anticancer Activity of Withanolide-Based Glucose Transporter Protein 1 Inhibitors from Physalis angulata var. villosa. [Abstract]

2024 Jan 26;87(1):2-13. PMID: 38117981
J Cancer

PLEKHG2 Promotes NSCLC Cell Growth by Increasing Glycolysis via Activated PI3K/AKT Pathway. [Abstract]

2023 Oct 30;14(18):3550-3560. PMID: 38021149
Biochem Biophys Res Commun

Effects of intracellular iron overload on cell death and identification of potent cell death inhibitors. [Abstract]

2018 Sep 3;503(1):297-303. PMID: 29890135
Reprod Biol

Phloretin enhances the efficiency of sheep oocyte IVM and early embryonic development by bolstering antioxidant defenses, inhibiting apoptosis, and promoting development. [Abstract]

2025 Nov 14;26(1):101102. PMID: 41240406
Res Sq

2026 Mar 4.
SSRN

2023 Jun 15.

溶剤 & 溶解度

体外:

DMSO : 50 mg/mL (182.30 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : < 0.1 mg/mL (insoluble)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

3.6460 mL

18.2302 mL

36.4604 mL

5 mM

0.7292 mL

3.6460 mL

7.2921 mL

10 mM

0.3646 mL

1.8230 mL

3.6460 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

モル濃度計算機

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

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分子量分子量

希釈計算機

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体積 _(開始) 体積 _(開始)

濃度 _(終了) 濃度 _(終了)

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体内:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (9.12 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (9.12 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (9.12 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

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The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

純度とドキュメンテーション

バッチを選択:

純度: 99.98%

Assay: 99.98%

ee.: 99.98%

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参考文献

[1]. Kasahara T, Kasahara M. Expression of the rat GLUT1 glucose transporter in the yeast Saccharomyces cerevisiae. Biochem J. 1996 Apr 1;315 ( Pt 1):177-82. [Content Brief]

[2]. Luo H, et al. Phloretin induces apoptosis of BEL-7402 cells in vitro. Nan Fang Yi Ke Da Xue Xue Bao. 2008 Jul;28(7):1249-51. [Content Brief]

[3]. Huang WC, et al. Phloretin and phlorizin promote lipolysis and inhibit inflammation in mouse 3T3-L1 cells and in macrophage-adipocyte co-cultures.Mol Nutr Food Res. 2013 Oct;57(10):1803-13. [Content Brief]

[4]. Wang SP, et al. Potent Antiarthritic Properties of Phloretin in Murine Collagen-Induced Arthritis.Evid Based Complement Alternat Med. 2016;2016:9831263. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	3.6460 mL	18.2302 mL	36.4604 mL	91.1511 mL
	5 mM	0.7292 mL	3.6460 mL	7.2921 mL	18.2302 mL
	10 mM	0.3646 mL	1.8230 mL	3.6460 mL	9.1151 mL
	15 mM	0.2431 mL	1.2153 mL	2.4307 mL	6.0767 mL
	20 mM	0.1823 mL	0.9115 mL	1.8230 mL	4.5576 mL
	25 mM	0.1458 mL	0.7292 mL	1.4584 mL	3.6460 mL
	30 mM	0.1215 mL	0.6077 mL	1.2153 mL	3.0384 mL
	40 mM	0.0912 mL	0.4558 mL	0.9115 mL	2.2788 mL
	50 mM	0.0729 mL	0.3646 mL	0.7292 mL	1.8230 mL
	60 mM	0.0608 mL	0.3038 mL	0.6077 mL	1.5192 mL
	80 mM	0.0456 mL	0.2279 mL	0.4558 mL	1.1394 mL
	100 mM	0.0365 mL	0.1823 mL	0.3646 mL	0.9115 mL

Keywords

Phloretin

25 Cited Publications

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MedChemExpress（MCE）の使用を引用している文献 Phloretin

SGLT アイソフォーム固有の製品をすべて表示

Endogenous Metabolite アイソフォーム固有の製品をすべて表示

GLUT アイソフォーム固有の製品をすべて表示

生物活性

化学情報

Publications (25)

溶剤 & 溶解度

純度とドキュメンテーション

参考文献

Help & FAQs

Complete Stock Solution Preparation Table

Phloretin 関連分類

製品番号: HY-N0142