TP-064
Based on 9 publication(s) in Google Scholar
TP-064, a chemical probe, is a potent and selective proteinarginine methyltransferase 4 (PRMT4; CARM1) inhibitor (IC50 <10 nM). TP-064 inhibits dimethylation of BAF155 (IC50 of 340 nM) and MED12 (IC50 of 43 nM). TP-064 is inactive against the other family members except for PRMT6 (IC50 of 1.3 μM). TP-064 has anticancer activities.
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- Purity: 99.29%
- CAS No.: 2080306-20-1
- 화학식: C28H34N4O2
- 분자량:458.60
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보관:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
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용액&용해도
- 순도&문서
- References
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) TP-064
More- Nat Commun. 2026 Feb 12;17(1):1214. [Abstract]
- Cell Death Differ. 2022 Oct;29(10):1982-1995. [Abstract]
- Redox Biol. 2024 Sep 6:76:103344. [Abstract]
- Cell Commun Signal. 2025 Oct 2;23(1):409. [Abstract]
- Dev Cell. 2024 May 31:S1534-5807(24)00330-7. [Abstract]
- Diabetes. 2023 Aug 1;72(8):1095-1111. [Abstract]
- Cell Rep. 2024 Jul 9;43(7):114459. [Abstract]
- Mol Med. 2025 Oct 31;31(1):322. [Abstract]
- Sci Rep. 2026 Jun 4. [Abstract]
All Histone Methyltransferase Isoforms
More
Biological Activity
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PRMT4 <10 nM (IC50) |
PRMT6 1300 μM (IC50) |
CARM1 |
TP-064 (1 μM; 72 hours) treatment reduces the proportion of NCI-H929 cells in S and G2/M phases while increasing the G1 phase fraction[1].
TP-064 (0.03-3 μM; 72 hours) treatment reduces dimethyl-BAF155 level in a dose-dependent manner in both TP-064-sensitive and -insensitive cells[1].
TP-064 (10 nM-10 μM; 6 days) treatment inhibits the growth of NCI-H929, RPMI8226, and MM.1R cells in a dose-dependent manner, but had no effect on acute myeloid leukemia, colon cancer, or lung cancer cell lines[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:NCI-H929 cells
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Concentration:1 μM
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Incubation Time:72 hours
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Result:Induced G1 cell cycle arrest in NCI-H929 cells.
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Cell Line:NCI-H929, KMS-27 and U266B1 cells
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Concentration:0.03 μM, 0.1 μM, 0.3 μM, 1 μM, 3 μM
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Incubation Time:72 hours
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Result:Dimethyl-BAF155 level was reduced.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 2080306-20-1
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Appearance Solid
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분자량 458.60
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화학식 C28H34N4O2
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Color White to light yellow
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SMILES
O=C(C1=CC=CC(OC2=CC=CC=C2)=C1)N(CC3=CC(C4CCN(CC4)CCNC)=NC=C3)C
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선적
Room temperature in continental US; may vary elsewhere.
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보관
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (9)
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Journal Impact Factor
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Most Recent
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Nat Commun
Human iPSC-based Modeling of Pulmonary Fibrosis Reveals p300/CBP Inhibition Suppresses Alveolar Transitional Cell State. [Abstract]2026 Feb 12;17(1):1214. PMID: 41680175 -
Cell Death Differ
PRMT4 promotes ferroptosis to aggravate doxorubicin-induced cardiomyopathy via inhibition of the Nrf2/GPX4 pathway. [Abstract]2022 Oct;29(10):1982-1995. PMID: 35383293 -
Redox Biol
2024 Sep 6:76:103344. PMID: 39265499 -
Cell Commun Signal
2025 Oct 2;23(1):409. PMID: 41039590 -
Dev Cell
Cancer-specific epigenome identifies oncogenic hijacking by nuclear factor I family proteins for medulloblastoma progression. [Abstract]2024 May 31:S1534-5807(24)00330-7. PMID: 38834071 -
Diabetes
2023 Aug 1;72(8):1095-1111. PMID: 37216643 -
Cell Rep
2024 Jul 9;43(7):114459. PMID: 38985674 -
Mol Med
Context-specific applications of CARM1 inhibitors: functional profiles of EZM2302 and TP-064. [Abstract]2025 Oct 31;31(1):322. PMID: 41174476 -
Sci Rep
CircRNAs derived from the tyrosine phosphatase PTPN22 impact chemosensitivity in ALK-positive T-cell lymphomas. [Abstract]2026 Jun 4. PMID: 42243281
용액&용해도
DMSO : 125 mg/mL (272.57 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : ≥ 50 mg/mL (109.03 mM)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (4.54 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (4.54 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
순도&문서
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Data Sheet (274 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Kazuhide Nakayama, et al. TP-064, a potent and selective small molecule inhibitor of PRMT4 for multiple myeloma. Oncotarget. 2018 Apr 6;9(26):18480-18493. [Content Brief]
[2]. Yiheng Zhang, et al. PRMT4 inhibitor TP-064 inhibits the pro-inflammatory macrophage lipopolysaccharide response in vitro and ex vivo and induces peritonitis-associated neutrophilia in vivo. Biochim Biophys Acta Mol Basis Dis. 2021 Jul 24;1867(11):166212. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O / DMSO | 1 mM | 2.1805 mL | 10.9027 mL | 21.8055 mL | 54.5137 mL |
| 5 mM | 0.4361 mL | 2.1805 mL | 4.3611 mL | 10.9027 mL | |
| 10 mM | 0.2181 mL | 1.0903 mL | 2.1805 mL | 5.4514 mL | |
| 15 mM | 0.1454 mL | 0.7268 mL | 1.4537 mL | 3.6342 mL | |
| 20 mM | 0.1090 mL | 0.5451 mL | 1.0903 mL | 2.7257 mL | |
| 25 mM | 0.0872 mL | 0.4361 mL | 0.8722 mL | 2.1805 mL | |
| 30 mM | 0.0727 mL | 0.3634 mL | 0.7268 mL | 1.8171 mL | |
| 40 mM | 0.0545 mL | 0.2726 mL | 0.5451 mL | 1.3628 mL | |
| 50 mM | 0.0436 mL | 0.2181 mL | 0.4361 mL | 1.0903 mL | |
| 60 mM | 0.0363 mL | 0.1817 mL | 0.3634 mL | 0.9086 mL | |
| 80 mM | 0.0273 mL | 0.1363 mL | 0.2726 mL | 0.6814 mL | |
| 100 mM | 0.0218 mL | 0.1090 mL | 0.2181 mL | 0.5451 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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