UCN-02
Based on 1 Customer Validation
UCN-02 (7-epi-Hydroxystaurosporine) is a selective protein kinase C (PKC) inhibitor with a PKC IC50 value of 62 nM and a PKA IC50 value of 0.25 μM. UCN-02 can be produced by Streptomyces strain N-126. UCN-02 can be used for the research of breast carcinoma.
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- Purity : 95%
- CAS No.: 121569-61-7
- 화학식: C28H26N4O4
- 분자량:482.53
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보관:Pure form -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Protocol
- 순도&문서
- References
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Help & FAQs
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Biological Activity
제품 설명
IC50 & Target
IC50: 62 nM (PKC), 250 nM (PKA)[2]
In Vitro
UCN-02 (0.25-0.37 μg/mL; 48-72 h) is produced by Streptomyces strain N-126[1].
UCN-02 inhibits purified protein kinase C (PKC) with an IC50 of 0.062 μM[2].
UCN-02 inhibits purified protein kinase A (PKA) with an IC50 of 0.25 μM[2].
UCN-02 (72 h) inhibits the growth of HeLa S3 cells[2].
UCN-02 (5-6 days) inhibits the growth of human breast carcinoma cell lines MDA-MB468 (IC50 = 100 nM), H85787 (IC50 = 20 nM), SK-BR-3 (IC50 = 200 nM), MDA-MB453 (IC50 = 350 nM), and MCF-7 (IC50 = 150 nM)[3].
UCN-02 (24 h-6 days) causes exposure-dependent growth inhibition in MDA-MB468 and MCF-7 human breast carcinoma cells[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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CAS No. 121569-61-7
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Appearance Liquid (Density: 1.63±0.1 g/cm3)
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분자량 482.53
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화학식 C28H26N4O4
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Color Colorless to light yellow
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SMILES
C[C@]12N3C4=C(C5=CC=CC=C53)C([C@@H](NC6=O)O)=C6C7=C4N([C@](C[C@H]([C@H]2OC)NC)([H])O1)C8=CC=CC=C78
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Synonyms
7-epi-Hydroxystaurosporine
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Structure Classification
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Initial Source
Streptomyces sp. N-126.
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선적
Room temperature in continental US; may vary elsewhere.
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보관
Pure form -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Protocol
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
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Breast Cancer Modeling
Breast cancer is a heterogeneous cancer, and it has been distinguished into four subtypes: luminal A, luminal B, HER2-positive and basal-like. Molecular mutations, epigenetic alterations, hormone exposure and immune microenvironment are related to the progression of breast cancer.
순도&문서
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Data Sheet (281 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Takahashi I, et al. UCN-01 and UCN-02, new selective inhibitors of protein kinase C. I. Screening, producing organism and fermentation. J Antibiot (Tokyo). 1989 Apr;42(4):564-70. [Content Brief]
[2]. Takahashi I, et al. UCN-01 and UCN-02, new selective inhibitors of protein kinase C. II. Purification, physico-chemical properties, structural determination and biological activities. J Antibiot (Tokyo). 1989 Apr;42(4):571-6. [Content Brief]
[3]. Seynaeve CM, et al. Cell cycle arrest and growth inhibition by the protein kinase antagonist UCN-01 in human breast carcinoma cells. Cancer Res. 1993;53(9):2081-2086. [Content Brief]
[4]. Seynaeve CM, et al. Differential inhibition of protein kinase C isozymes by UCN-01, a staurosporine analogue. Mol Pharmacol. 1994;45(6):1207-1214. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
UCN-02 Related Classifications
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