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Art. -Nr.: HY-N21972

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Longilactone

Longilactone is a quassinoid diterpenoid isolated from the roots of Eurycoma longifolia. Longilactone induces apoptosis in MCF-7 cells via the extrinsic caspase pathway, activating the cleavage of caspase-8, caspase-7 and PARP. Longilactone can be used in research related to breast cancer and human lung cancer.

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CAS. Nr.: 129587-09-3
Formel: C₁₉H₂₆O₇
Molecular Weight:366.41

Speicherung:

Please store the product under the recommended conditions in the Certificate of Analysis.

Longilactone Chemical Structure

CAS. Nr.: 129587-09-3

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Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- Cellular Effect
- In Vitro
Chemical Information
Reinheit & Dokumentation
Verweise
Calculators
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

IC₅₀ & Target^[1]

Caspase-8

Caspase-7

PARP

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Cellular Effect

Cell Line	Type	Value	Description	References
MCF7	IC₅₀	0.53 μg/mL	Cytotoxic activity against human breast cancer MCF-7 cells assessed via Sulforhodamine B (SRB) assay following 72 hrs incubation. Cytotoxic activity against human breast cancer MCF-7 cells assessed via Sulforhodamine B (SRB) assay following 72 hrs incubation.	article1379247682_Muhamad%20et%20al.pdf
A549	ED₅₀	4.6 μg/mL	Cytotoxicity against human lung cancer A-549 cells. Cytotoxicity against human lung cancer A-549 cells.	14738962
MCF7	ED₅₀	6.1 μg/mL	Cytotoxicity against human breast cancer MCF-7 cells. Cytotoxicity against human breast cancer MCF-7 cells.	14738962

In Vitro

Longilactone (0.32-20 µg/mL; 72 h) potently inhibits the viability of human breast cancer MCF-7 cells, with an IC₅₀ of 0.53 µg/mL after 72 h of treatment^[1].
Longilactone (compound 36) exhibits cytotoxic activity against human lung cancer (A-549) cells with an ED₅₀ value of 4.6 μg/mL; it also shows cytotoxic activity against human breast cancer (MCF-7) cells with an ED₅₀ value of 6.1 μg/mL^[3].
Longilactone (5 µg/mL; 12-72 h) induces time-dependent apoptosis in human breast cancer MCF-7 cells^[1].
Longilactone (5 µg/mL; 72 h) induces apoptosis in human breast cancer MCF-7 cells^[1].
Longilactone (5 µg/mL; 0-72 h) activates caspase-8 in a time-dependent manner in human breast cancer MCF-7 cells, but has no effect on the activity of caspase-9^[1].
Longilactone (5 µg/mL; 0-72 h) upregulates the levels of activated caspase-8, activated caspase-7 and cleaved PARP in a time-dependent manner, activates the extrinsic apoptotic pathway in human breast cancer MCF-7 cells, and does not alter the expression of Bax and Bcl-2 or activate caspase-9^[1].
Longilactone exhibits no significant anti-HIV activity in H9 lymphocytes^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Cytotoxicity Assay^[1]

Cell Line:

human breast cancer MCF-7 cells
Concentration:

0.32, 0.16, 0.8, 4, 20 µg/mL
Incubation Time:

72 h
Result:

Exerted strong cytotoxic activity on MCF-7 cells, with an IC₅₀ of 0.53 µg/mL.

Apoptosis Analysis^[1]

Cell Line:

human breast cancer MCF-7 cells
Concentration:

5 µg/mL
Incubation Time:

12, 24, 48, 72 h
Result:

Induced typical apoptotic nuclear morphology changes including fragmentation, hypercondensation, and shrinkage after 48 h incubation.
Increased the percentage of apoptotic MCF-7 cells in a time-dependent manner, reaching 74.3% after 72 h.

Western Blot Analysis^[1]

Cell Line:

human breast cancer MCF-7 cells
Concentration:

5 µg/mL
Incubation Time:

12, 24, 48, 72 h
Result:

Increased levels of pro-caspase-8 (55 kDa), active caspase-8 (29 kDa), active caspase-7 (20 kDa), and cleaved poly (ADP-ribose) polymerase (PARP, 89 kDa) in a time-dependent manner.
Did not alter basal levels of Bax (20 kDa) and Bcl-2 (26 kDa), and active caspase-9 was not detected.

Chemical Information

CAS. Nr. 129587-09-3
Molecular Weight 366.41
Formel C₁₉H₂₆O₇
SMILES

C[C@]12[C@@]3([H])[C@@]4([C@@](C(C)=CC([C@H]4O)=O)([H])[C@H]([C@@]1([H])OC([C@@]2([H])[C@H]([C@H]([C@@H]3O)O)C)=O)O)C
Structure Classification
- Others
Initial Source
- Plants
- Simaroubaceae
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung

Please store the product under the recommended conditions in the Certificate of Analysis.

Reinheit & Dokumentation

Handling Instructions (2659 KB)

Verweise

[1]. Shamsul Muhamad, et al. Induction of apoptosis in MCF-7 via the Caspase pathway by longilactone from Eurycoma longifolia Jack. Research In Pharmaceutical Biotechnology Vol. 3(1), pp. 1-10, January 2011.ISSN 2141-2324

[2]. Han YM, et al. Simultaneous quantitation of six major quassinoids in Tongkat Ali dietary supplements by liquid chromatography with tandem mass spectrometry. J Sep Sci. 2015 Jul;38(13):2260-6.

[3]. Kuo PC, et al. Cytotoxic and antimalarial constituents from the roots of Eurycoma longifolia. Bioorganic & medicinal chemistry. 2004 Feb 01;12(3):537-44. [Content Brief]

Calculators

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Bioz

Keywords

MOQ

Minimum order quantity

100 mg

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Biologische Aktivität
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- IC₅₀ & Target
- In Vitro
Chemical Information
Reinheit & Dokumentation
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Longilactone

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